本文選題：堿性成纖維細(xì)胞生長因子　切入點(diǎn)：氯胺酮　出處：《重慶醫(yī)科大學(xué)學(xué)報(bào)》2016年01期

【摘要】：目的 :探討鞘內(nèi)間斷多次給予N-甲基-D-天門冬氨酸(N-methyl-D-aspartate,NMDA)受體拮抗劑對坐骨神經(jīng)分支選擇性損傷模型(spared nerve injury,SNI)大鼠神經(jīng)病理性疼痛的緩解作用及其可能的機(jī)制。方法:雄性SD大鼠72只,體質(zhì)量240~260 g,采用隨機(jī)數(shù)字表法,將大鼠隨機(jī)分為6組(n=12):假手術(shù)組(S組)、坐骨神經(jīng)分支選擇性損傷組(NP組)、氯胺酮低劑量組(K1組)、氯胺酮高劑量組(K2組)、MK-801低劑量組(M1組)、MK-801高劑量組(M2組)。S組僅暴露坐骨神經(jīng),NP組、K1組、K2組、M1組、M2組制備坐骨神經(jīng)分支選擇性損傷模型。從術(shù)后1 d開始,K1組、K2組、M1組、M2組鞘內(nèi)分別注射氯胺酮50μg、氯胺酮100μg、MK-801 5μg和MK-801 10μg,容量均為10μl,S組和NP組鞘內(nèi)注射生理鹽水10μl,1次/d,連續(xù)給藥7 d。分別于術(shù)前1 d和術(shù)后1~7 d,進(jìn)行疼痛行為學(xué)觀察和機(jī)械刺激縮足反射閾值(paw withdrawal mechanical threshold,PWMT)測定。所有大鼠均于術(shù)后第7天處死,免疫組化觀察大鼠脊髓L4~6節(jié)段背角膠質(zhì)纖維酸性蛋白(glial fibrillary acidic protein,GFAP)和堿性成纖維細(xì)胞生長因子(basic fibroblast growth factor,b FGF)表達(dá)變化,Western blot檢測脊髓L4~6節(jié)段b FGF蛋白含量變化。結(jié)果:術(shù)后第7天給藥前和給藥后,NP組術(shù)側(cè)PWMT較S組明顯降低(P=0.000),K1組、K2組、M1組和M2組術(shù)側(cè)PWMT較NP組明顯升高(P=0.000)。免疫組化結(jié)果顯示,NP組術(shù)側(cè)b FGF、GFAP蛋白表達(dá)量較S組明顯升高(P=0.000);K1組、K2組、M1組和M2組bFGF、GFAP蛋白表達(dá)量較NP組明顯降低(P=0.000)。Western blot結(jié)果顯示,NP組b FGF蛋白表達(dá)量較S組明顯升高(P=0.000);K1組、K2組、M1組和M2組b FGF蛋白表達(dá)量較NP組明顯降低(P=0.000)。結(jié)論:鞘內(nèi)間斷多次給予NMDA受體拮抗劑可緩解SNI引起的大鼠痛覺過敏,其機(jī)制可能與抑制b FGF蛋白表達(dá)及星形膠質(zhì)細(xì)胞活化有關(guān)。
[Abstract]:Objective: to investigate the effect of N-methyl-Daspartate N-methyl-D-aspartate receptor antagonist on neuropathic pain induced by selective injury of sciatic nerve branches in rats with intrathecal intermittent administration of N-methyl-D-aspartate and its possible mechanism. 72 male SD rats, The body mass is 240 ~ 260g, and the random digital table method is used. Rats were randomly divided into 6 groups: sham operation group (S group), sciatic nerve branch selective injury group (NP-group), ketamine low-dose group (K1), ketamine high-dose group (K2), MK-801 low-dose group (M1 group), MK-801 high dose group (M2 group), group S (group S) only exposed to MK-801, MK-801, MK-801, MK-801, MK-801 and MK-801. The selective injury model of sciatic nerve branching was established in group K1, group K1, group K2, group M 1, group M 2, group K2, group K2, group K2, group K2, group K2, group K2, group K2, group K2, group K1, group K1, group K1, group K1, group K1, group K1, group K1, group K1, group K1, group K1, group K1, group K1, group K2, group K1, group K1, group K1, group K2, group K1, group K1, group K2. In NP group, intrathecal injection of normal saline 10 渭 l / d was given continuously for 7 days. The pain behavior and mechanical stimulation reflex threshold of paw withdrawal mechanical threshold were measured 1 day before operation and 1 day after operation. All the rats were killed on the 7th day after operation. Immunohistochemical study on the expression of glial fibrillary acidic protein in dorsal horn of rat spinal cord L4 ~ (6) and basic fibroblast growth factor-B (FGFs). Western blot was used to detect the protein content of b FGF in L4 ~ (6) segment of spinal cord. Results: after operation, the content of b FGF in L4 ~ (6) segment of spinal cord was determined by Western blot. On the 7th day after administration, the PWMT of the operative side in the NP group was significantly lower than that in the S group. The PWMT in the operative side of the K2 group and the M2 group was significantly higher than that of the NP group. Immunohistochemical results showed that the expression of bFGFGFAP protein in the operative side of the NP group was significantly higher than that in the S group, and the expression of PWMT in the operative side of the NP group was significantly higher than that in the S group. The results of Western blot showed that the expression of bFGFGFAP in K2 group was significantly higher than that in group S group (P < 0.05). Conclusion: the expression of bFGFGFAP protein in group M 1 and group M 2 is significantly lower than that in group NP. Conclusion: the results showed that the expression of b FGF protein was significantly higher in group C than in group C (P < 0.05). Conclusion: the expression of b FGF protein in group M 1 and group M 2 is significantly lower than that in group NP (P < 0.01). Conclusion: the expression of b FGF in group C is significantly higher than that in group S. Conclusion:. Repeated intrathecal administration of NMDA receptor antagonists alleviated hyperalgesia induced by SNI in rats. The mechanism may be related to the inhibition of the expression of b FGF protein and the activation of astrocytes.
【作者單位】： 重慶醫(yī)科大學(xué)附屬第一醫(yī)院麻醉科;
【基金】：重慶市衛(wèi)生局醫(yī)學(xué)科研計(jì)劃資助項(xiàng)目(編號:2012-1-018) 重慶市醫(yī)學(xué)重點(diǎn)學(xué)科(編號:渝衛(wèi)科教[2007]2號) 衛(wèi)生部國家臨床重點(diǎn)專科建設(shè)經(jīng)費(fèi)資助項(xiàng)目(編號:財(cái)社[2011]170號)
【分類號】：R741

【相似文獻(xiàn)】

相關(guān)期刊論文 前5條

1 單巍松，，王朝暉;NMDA受體拮抗劑與驚厥治療[J];國外醫(yī)學(xué)(兒科學(xué)分冊);1995年03期

2 張春芬;谷氨酸NMDA受體拮抗劑對帕金森病的治療作用[J];國外醫(yī)藥.合成藥.生化藥.制劑分冊;1997年01期

3 羅雪;米健;;側(cè)腦室微量注射NMDA受體拮抗劑對神經(jīng)病理疼痛的作用[J];現(xiàn)代醫(yī)藥衛(wèi)生;2011年20期

4 潘燕燕;龔珊;虞獻(xiàn)民;蔣星紅;;下丘腦弓狀核微量注射NMDA受體拮抗劑和蛋白激酶抑制劑對神經(jīng)病理性痛覺過敏的抑制作用[J];蘇州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2010年02期

5 ;[J];;年期

相關(guān)會議論文 前1條

1 曹仁存;王慶松;林杭;吳渝憲;王偉文;;NMDA受體拮抗劑和Ca2+拮抗劑聯(lián)合治療反復(fù)缺血性腦損害[A];中華醫(yī)學(xué)會第十三次全國神經(jīng)病學(xué)學(xué)術(shù)會議論文匯編[C];2010年

本文編號：1671807