【摘要】：背景:已有文獻報道,間充質干細胞可通過分泌大量生物活性因子有效減輕損傷的心臟、肺臟、腎臟等器官纖維化的發(fā)生。目的:觀察骨髓間充質干細胞條件培養(yǎng)液對正常成纖維細胞和瘢痕成纖維細胞的影響及作用機制。方法:制備骨髓間充質干細胞條件培養(yǎng)液,將其作用于正常成纖維細胞和增生性瘢痕成纖維細胞,采用ELISA檢測轉化生長因子β的分泌水平,RT-PCR檢測TGF-β/Smad信號通路相關基因Smad7的表達,比色法檢測各組細胞上清液中羥脯氨酸的含量。結果與結論:(1)骨髓間充質干細胞條件培養(yǎng)液對增生性瘢痕成纖維細胞轉化生長因子β的分泌、膠原的合成有顯著抑制作用(P0.01),并且明顯增強Smad7基因的表達(P0.01);(2)骨髓間充質干細胞條件培養(yǎng)液對正常成纖維細胞的影響較小;(3)結果表明,骨髓間充質干細胞條件培養(yǎng)液對增生性瘢痕成纖維細胞具有顯著抑制作用,為干細胞防治皮膚瘢痕提供基礎理論支持。
[Abstract]:Background: it has been reported that mesenchymal stem cells can effectively reduce the occurrence of fibrosis in injured heart, lung, kidney and other organs by secreting a large number of bioactive factors. Aim: to observe the effect and mechanism of conditioned medium of bone marrow mesenchymal stem cells on normal fibroblasts and scar fibroblasts. Methods: the conditioned medium of bone marrow mesenchymal stem cells was prepared and applied to normal fibroblasts and hypertrophic scar fibroblasts. The secretion of transforming growth factor 尾 (TGF 尾) was detected by ELISA. The expression of Smad7 related to TGF- 尾 / Smad signaling pathway was detected by RT-PCR, and the content of hydroxyproline in the supernatant of each group was detected by colorimetric assay. Results and conclusion: (1) conditioned medium of bone marrow mesenchymal stem cells significantly inhibited the secretion of TGF-尾 and collagen synthesis in hypertrophic scar fibroblasts (P0.01). And significantly increased the expression of Smad7 gene (P0.01); (2) the conditioned medium of bone marrow mesenchymal stem cells had little effect on normal fibroblasts; (3) the results showed that the conditioned medium of bone marrow mesenchymal stem cells had significant inhibitory effect on hypertrophic scar fibroblasts, which provided the basic theory support for the prevention and treatment of skin scar by stem cells.
【作者單位】： 牡丹江醫(yī)學院醫(yī)藥研究中心;牡丹江醫(yī)學院紅旗醫(yī)院眼科;牡丹江醫(yī)學院病理學教研室;牡丹江醫(yī)學院解剖學教研室;牡丹江醫(yī)學院紅旗醫(yī)院檢驗科;
【基金】：黑龍江省普通本科高等學校青年創(chuàng)新人才培養(yǎng)計劃(UNPYSCT-2015109)~~
【分類號】：R641
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本文編號：2456116