【摘要】：目的：通過(guò)觀察大鼠ICH后小膠質(zhì)細(xì)胞（OX-42）ǎNF-κBp65ǎBDNFǎSYPǎCaspase3的動(dòng)態(tài)表達(dá)及安腦丸對(duì)其表達(dá)的干預(yù)作用，，探討安腦丸對(duì)ICH后繼發(fā)性腦損傷的保護(hù)作用機(jī)制 方法：將Sprague-Dawley(SD)系雄性大鼠190只隨機(jī)分為正常組ǎ假手術(shù)組ǎICH模型組（模型組）ǎ安腦丸干預(yù)組(安腦丸組)；后三組組內(nèi)隨機(jī)分為術(shù)后12hǎ1dǎ2dǎ4dǎ7dǎ10d六個(gè)時(shí)間點(diǎn)；按Rosenberg法建立大鼠ICH模型，安腦丸組每天上下午各灌胃1mg/ml安腦丸10ml/kg一次分別于術(shù)后各時(shí)間點(diǎn)對(duì)大鼠進(jìn)行神經(jīng)功能缺損評(píng)分；免疫組化方法檢測(cè)大鼠腦組織中小膠質(zhì)細(xì)胞ǎNF-κBp65ǎBDNFǎSYNǎCaspase3陽(yáng)性細(xì)胞表達(dá)及Western blot法檢測(cè)大鼠腦組織BDNFǎCaspase3蛋白表達(dá)情況 結(jié)果：安腦丸組大鼠神經(jīng)功能缺損評(píng)分低于模型組（P0.05）免疫組化法檢測(cè)結(jié)果顯示，正常組ǎ假手術(shù)組大鼠僅見少量OX42陽(yáng)性細(xì)胞表達(dá)，模型組ICH后第12h即可見血腫周圍有大量OX42陽(yáng)性細(xì)胞表達(dá)，第7-10d表達(dá)達(dá)到高峰，安腦丸組各時(shí)間點(diǎn)OX42陽(yáng)性細(xì)胞表達(dá)及活化都無(wú)模型組明顯（P0.01）；模型組ICH后第12hNF-κBp65表達(dá)明顯增多，第4d時(shí)NF-κBp65表達(dá)達(dá)到高峰，安腦丸組各時(shí)間點(diǎn)NF-κBp65表達(dá)均明顯少于模型組（P0.05）；模型組ICH后第12h BDNF表達(dá)明顯增高，第1d時(shí)達(dá)到高峰，安腦丸組各時(shí)間點(diǎn)表達(dá)都明顯高于模型組（P0.01）；ICH后第1dǎ2dǎ4dǎ7dǎ10d時(shí)安腦丸組SYP蛋白表達(dá)高于模型組，差異有統(tǒng)計(jì)學(xué)意義（P0.01）；模型組和安腦丸組ICH后第12h可見Caspase3大量表達(dá)，安腦丸組各時(shí)間點(diǎn)Caspase3陽(yáng)性細(xì)胞表達(dá)明顯少于模型組（P0.01） Westernblot檢測(cè)結(jié)果示，模型組在ICH后第12h時(shí)BDNF蛋白的表達(dá)顯著增多，在第1d時(shí)達(dá)到高峰，安腦丸組各時(shí)間點(diǎn)BDNF蛋白表達(dá)均明顯高于模型組（P0.01）；模型組ICH后第12h時(shí)Caspase3蛋白表達(dá)明顯增高，第1d時(shí)達(dá)高峰，安腦丸組各時(shí)間點(diǎn)Caspase3表達(dá)均明顯低于模型組（P0.01） 結(jié)論：ICH后小膠質(zhì)細(xì)胞即被激活及NF-κBp65ǎBDNFǎSYN與Caspase3高表達(dá)，安腦丸可通過(guò)顯著減少ICH后小膠質(zhì)細(xì)胞活化ǎNF-κBp65及Caspase3高表達(dá)，增加BDNF及SYN的表達(dá)而對(duì)出血腦組織具有一定的保護(hù)作用
[Abstract]:Objective: to observe the dynamic expression of microglia (OX-42) NF- kappa Bp65 / BDNF / SYP-Caspase-3 after ICH in rats and the intervention effect of Annao Pill on the expression. To explore the protective mechanism of Annao Pill on secondary brain injury after ICH methods: Sprague-Dawley (SD) male rats were randomly divided into normal group (sham operation group) ICH model group (model group) and Anao pill intervention group (Annao pill group). The rats in the latter three groups were randomly divided into 6 time points, 12 h, 2 d, 4 d, 7 d and 10 d, according to Rosenberg method to establish the rat ICH model. The rats in the Annao pill group were given 1mg/ml Annao Pill 10ml/kg once a day in the morning and afternoon, and the neurological impairment scores were evaluated at each time point after the operation. Immunohistochemical method was used to detect the expression of NF- kappa Bp65 BDNF and SYN Caspase 3 positive cells in rat brain tissue and Western blot method was used to detect the expression of BDNFCaspase3 protein in rat brain tissue. The results showed that the score of neural function defect in the Annao pill group was lower than that in the model. In group A (P0.05), the results of immunohistochemistry showed that, There were only a few OX42 positive cells in the sham-operated rats in the normal group, and a large number of OX42 positive cells were found around the hematoma at 12 h after ICH in the model group, and reached the peak on the 7th-10th day. There was no significant difference in the expression and activation of OX42 positive cells in the Annao pill group at each time point (P0.01). The expression of NF-魏 Bp65 in the model group increased significantly at the 12th hour after ICH, and reached the peak on the 4th day. The expression of NF- 魏 Bp65 in the Annao pill group at each time point was significantly lower than that in the model group (P0.05). The expression of BDNF in the model group was significantly higher than that in the model group at the 12th hour after ICH and reached the peak on the 1st day. The expression of BDNF in the Annao pill group was significantly higher than that in the model group (P0.01). The expression of SYP protein in Annao pill group was significantly higher than that in model group on the 1st day, 2nd day, 4th day, 7th day and 10th day after ICH (P0.01). Significant expression of Caspase3 was observed at the 12th hour after ICH in the model group and Annao pill group. The expression of Caspase3 positive cells in the Annao pill group was significantly lower than that in the model group (P0.01) at each time point. The expression of BDNF protein in the model group increased significantly at the 12th hour after ICH. On the 1st day, the expression of BDNF protein in Annao pill group was significantly higher than that in model group (P0.01). The expression of Caspase3 protein increased significantly at the 12th hour after ICH in the model group, and reached the peak on the 1st day. The expression of Caspase3 in the Annao pill group was significantly lower than that in the model group (P0.01). Conclusion: microglia were activated after ICH and the expression of NF- kappa Bp65 / BDNF syn and Caspase3 was higher than that in the control group (P < 0.01). Annao Pill can significantly reduce the expression of NF- 魏 Bp65 and Caspase3 in microglia activated by ICH, increase the expression of BDNF and SYN, and protect the brain tissue from hemorrhage.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R651.15

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李光勤,董為偉;大鼠局灶性腦缺血后白細(xì)胞浸潤(rùn)及強(qiáng)力霉素干預(yù)效果的觀察[J];中華醫(yī)學(xué)雜志;1998年11期

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