大黃對(duì)嚴(yán)重?zé)齻笫竽c源性真菌感染防治作用的研究
發(fā)布時(shí)間：2019-02-28 12:22
【摘要】：目的：探討大黃對(duì)嚴(yán)重?zé)齻笫竽c源性真菌感染的防治作用。 方法：選取健康Wistar大鼠72只,隨機(jī)分成A、B、C三組,并分別以7×108個(gè)/ml濃度的白色念珠菌菌液灌胃,造成腸源性真菌感染模型。其中,A組為健康鼠菌液灌胃,B組為燙傷鼠菌液灌胃,C組為燙傷鼠菌液灌胃并在5分鐘后以50%大黃液按5ml/kg灌胃。之后分不同時(shí)段(6h,12h,24h,48h)將大鼠處死,并立即抽取大鼠心臟靜脈血液做肝功能和腎功能的檢測(cè)；取各組大鼠肺臟組織和腸粘膜組織,通過(guò)ELISA法分別檢測(cè)髓過(guò)氧化物酶和熱休克蛋白-70；采用實(shí)時(shí)熒光定量PCR法檢測(cè)各組大鼠肺臟、肝臟、腸系膜淋巴結(jié)和腎臟組織中白色念珠菌含量的CT值。 結(jié)果：①肝功能靜脈血檢測(cè)結(jié)果B組ALT和AST在同一時(shí)間段都較A組明顯升高,P0.001；C組較B組在同一時(shí)間段下降明顯,P值分別小于0.01和0.05,有統(tǒng)計(jì)學(xué)意義。②腎功能靜脈血檢測(cè)結(jié)果A組各時(shí)間段BUN和Cr都處于正常水平；B組與A組比較,6小時(shí)和12小時(shí)時(shí)間段無(wú)顯示明顯升高,P0.05,無(wú)統(tǒng)計(jì)學(xué)意義,24小時(shí)和48小時(shí)時(shí)間段升高明顯,P0.001,有顯著性差異；C組與B組比較,BUN和Cr在6小時(shí)和12小時(shí)時(shí)間段無(wú)顯示明顯升高,P0.05,無(wú)統(tǒng)計(jì)學(xué)意義,24小時(shí)和48小時(shí)時(shí)間段升高明顯,P值分別小于0.01和0.05,有顯著性差異。③肺部MPO結(jié)果B組較A組在同一時(shí)間段增高很多,P0.001,有統(tǒng)計(jì)學(xué)意義；而C組較B組在同一時(shí)間段下降明顯,P0.001。④腸道HSP-70結(jié)果B組較A組明顯升高,P0.001；而C組較B組也有不同程度升高,6小時(shí)段和24小時(shí)段P值均小于0.05,12小時(shí)段和48小時(shí)段P值均小于0.001。⑤熒光定量PCR法檢測(cè)結(jié)果肺臟組織A組48小時(shí)開(kāi)始檢測(cè)到含有白色念珠菌；B組從12小時(shí)段開(kāi)始檢測(cè)到含有白色念珠菌,且在24小時(shí)和48小時(shí)段CT值都較前一時(shí)間段下降,說(shuō)明菌含量在不斷上升,與A組同一時(shí)間段比較,P0.01,有顯著性差異；C組6小時(shí)和12小時(shí)時(shí)間段CT值大于35,沒(méi)有檢測(cè)到白色念珠菌,與B組比較,12小時(shí)段P0.01,24小時(shí)和48小時(shí)段P0.05,有統(tǒng)計(jì)學(xué)意義；肝臟組織中真菌檢測(cè)到的時(shí)間段在A組為12小時(shí),B組為6小時(shí),B組同一時(shí)間段與A組比較,CT值都下降明顯,P0.01,使用大黃后,CT值又有明顯升高,P0.05；腸系膜淋巴結(jié)的真菌含量檢測(cè)的CT值4個(gè)時(shí)間段都小于35,且隨著時(shí)間的推移,B組較A組的CT值下降明顯,P0.01,而C組則較B組有明顯上升,P0.05；腎臟組織中真菌含量檢測(cè)到的時(shí)間A組在12小時(shí),B組從6小時(shí)開(kāi)始檢測(cè)到,且較A組在同一時(shí)間段明顯下降,P0.01,而C組檢測(cè)到的真菌含量CT值較B組在同一時(shí)間段也是明顯升高,P0.05. 結(jié)論：嚴(yán)重?zé)齻?腸源性真菌感染的幾率升高,播散到機(jī)體其它臟器的范圍擴(kuò)大,肝、腎功能的受損程度加重,肺部MPO和腸道HSP-70的表達(dá)增高；早期應(yīng)用大黃,可以減輕燙傷大鼠的腸源性真菌感染的程度,縮小真菌向其它臟器播散的范圍,并使相關(guān)指標(biāo)降低。
[Abstract]:Objective: to investigate the preventive and therapeutic effects of rhubarb on intestinal fungal infection in severely burned rats. Methods: 72 healthy Wistar rats were randomly divided into three groups: group A, group B, and group C, and were fed with 7 脳 10 ~ 8 Candida albicans / ml respectively to establish intestinal fungal infection model. Group A, group B, group C, group A, group B, and group C, respectively, were perfused with 50% rhubarb solution according to 5ml/kg after 5 minutes. Then the rats were killed at different time points (6 h, 12 h, 24 h, 48 h), and the rat heart venous blood was immediately taken to detect the liver function and renal function. Myeloperoxidase (MPO) and heat shock protein-70 (HSP70) were detected by Elisa in lung and intestinal mucosa of rats in each group. The CT values of Candida albicans in lung, liver, mesenteric lymph node and kidney of rats in each group were measured by real-time fluorescence quantitative PCR. Results: (1) the levels of ALT and AST in group B were significantly higher than those in group A at the same time (P0.001). Compared with group B, the levels of BUN and Cr in group C were significantly lower than those in group B (P < 0. 01 and 0. 05, P < 0. 01 and 0. 05 respectively, P < 0. 01). Compared with group A, there was no significant difference between group B and group A in 6 hours and 12 hours, P 0.05, and no statistical significance. There was significant increase in 24 hours and 48 hours in group B, P 0.001, there was significant difference between group B and group A (P < 0.05). Compared with group B, there was no significant increase in BUN and Cr in group C at 6 h and 12 h, P 0.05, P < 0.05, P < 48 h, P < 0.05, P < 0.05. (3) the results of pulmonary MPO in group B were significantly higher than those in group A at the same time period (P0.001, P < 0.01), and there was significant difference between the two groups (P < 0.01). P0.001.4 intestinal HSP-70 results in group B were significantly higher than those in group A (P0.001 1), but in group C it was significantly lower than that in group B (P < 0.01). Group C was also higher than group B in varying degrees. P values were less than 0.05 in 6 hours and 24 hours, and less than 0.001.5 in 12 hours and 48 hours. The results of fluorescence quantitative PCR showed that Candida albicans was detected in group A at 48 hours. Candida albicans was detected in group B from the 12-hour period, and the CT values in 24-hour and 48-hour groups were lower than those in the previous one, indicating that the bacteria content was increasing continuously, and there was significant difference between group A and group A in the same period of time (P 0.01). There was no Candida albicans detected in group C with CT values greater than 35 in 6 hours and 12 hours. Compared with group B, 12 hours, 24 hours and 48 hours in group C, there was significant difference in CT values (P 0.01, 24 hours and 48 hours, P 0.05). The time period of fungus detection in liver tissue was 12 hours in group A and 6 hours in group B. Compared with group A, CT value in group B decreased significantly at the same time, P 0.01. After using rhubarb, CT value increased again, P 0.05; The CT values of fungi in mesenteric lymph nodes were all less than 35 in 4 time periods, and the CT values in group B were significantly lower than those in group A (P 0.01), while those in group C were significantly higher than those in group B (P 0.05), and the CT values in group B were significantly lower than those in group A (P 0.05). The time of detection of fungal content in kidney tissue was 12 hours in group A and 6 hours in group B, and it was significantly lower than that in group A at the same time period (P0.01, P < 0.01). The CT value of fungi detected in group C was significantly higher than that in group B at the same time (P 0.05). Conclusion: after severe burn, the incidence of intestinal fungal infection is increased, the spread to other organs of the body is expanded, the degree of liver and kidney damage is aggravated, and the expression of MPO and HSP-70 in the lungs is increased. Early application of rhubarb can reduce the degree of intestinal fungal infection in scalded rats, reduce the spread of fungi to other organs, and reduce the related indexes.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R965;R644
本文編號(hào)：2431797
[Abstract]:Objective: to investigate the preventive and therapeutic effects of rhubarb on intestinal fungal infection in severely burned rats. Methods: 72 healthy Wistar rats were randomly divided into three groups: group A, group B, and group C, and were fed with 7 脳 10 ~ 8 Candida albicans / ml respectively to establish intestinal fungal infection model. Group A, group B, group C, group A, group B, and group C, respectively, were perfused with 50% rhubarb solution according to 5ml/kg after 5 minutes. Then the rats were killed at different time points (6 h, 12 h, 24 h, 48 h), and the rat heart venous blood was immediately taken to detect the liver function and renal function. Myeloperoxidase (MPO) and heat shock protein-70 (HSP70) were detected by Elisa in lung and intestinal mucosa of rats in each group. The CT values of Candida albicans in lung, liver, mesenteric lymph node and kidney of rats in each group were measured by real-time fluorescence quantitative PCR. Results: (1) the levels of ALT and AST in group B were significantly higher than those in group A at the same time (P0.001). Compared with group B, the levels of BUN and Cr in group C were significantly lower than those in group B (P < 0. 01 and 0. 05, P < 0. 01 and 0. 05 respectively, P < 0. 01). Compared with group A, there was no significant difference between group B and group A in 6 hours and 12 hours, P 0.05, and no statistical significance. There was significant increase in 24 hours and 48 hours in group B, P 0.001, there was significant difference between group B and group A (P < 0.05). Compared with group B, there was no significant increase in BUN and Cr in group C at 6 h and 12 h, P 0.05, P < 0.05, P < 48 h, P < 0.05, P < 0.05. (3) the results of pulmonary MPO in group B were significantly higher than those in group A at the same time period (P0.001, P < 0.01), and there was significant difference between the two groups (P < 0.01). P0.001.4 intestinal HSP-70 results in group B were significantly higher than those in group A (P0.001 1), but in group C it was significantly lower than that in group B (P < 0.01). Group C was also higher than group B in varying degrees. P values were less than 0.05 in 6 hours and 24 hours, and less than 0.001.5 in 12 hours and 48 hours. The results of fluorescence quantitative PCR showed that Candida albicans was detected in group A at 48 hours. Candida albicans was detected in group B from the 12-hour period, and the CT values in 24-hour and 48-hour groups were lower than those in the previous one, indicating that the bacteria content was increasing continuously, and there was significant difference between group A and group A in the same period of time (P 0.01). There was no Candida albicans detected in group C with CT values greater than 35 in 6 hours and 12 hours. Compared with group B, 12 hours, 24 hours and 48 hours in group C, there was significant difference in CT values (P 0.01, 24 hours and 48 hours, P 0.05). The time period of fungus detection in liver tissue was 12 hours in group A and 6 hours in group B. Compared with group A, CT value in group B decreased significantly at the same time, P 0.01. After using rhubarb, CT value increased again, P 0.05; The CT values of fungi in mesenteric lymph nodes were all less than 35 in 4 time periods, and the CT values in group B were significantly lower than those in group A (P 0.01), while those in group C were significantly higher than those in group B (P 0.05), and the CT values in group B were significantly lower than those in group A (P 0.05). The time of detection of fungal content in kidney tissue was 12 hours in group A and 6 hours in group B, and it was significantly lower than that in group A at the same time period (P0.01, P < 0.01). The CT value of fungi detected in group C was significantly higher than that in group B at the same time (P 0.05). Conclusion: after severe burn, the incidence of intestinal fungal infection is increased, the spread to other organs of the body is expanded, the degree of liver and kidney damage is aggravated, and the expression of MPO and HSP-70 in the lungs is increased. Early application of rhubarb can reduce the degree of intestinal fungal infection in scalded rats, reduce the spread of fungi to other organs, and reduce the related indexes.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R965;R644
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