發(fā)布時間：2018-05-17 06:08

  本文選題：Ghrelin + 急性心肌梗死��； 參考：《重慶醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的探討Ghrelin對急性心肌梗死(AMI)大鼠心功能的影響及其可能機制。 方法成年雄性SD大鼠18只，結(jié)扎冠狀動脈前降支建立AMI模型。于建模后第4天，將存活的14只大鼠隨機分為模型組(AMI+NS組，，N=7)和處理組(AMI+Ghrelin組，N=7)，處理組按100μg/kg皮下注射Ghrelin，2次/d，間隔12h，共2周，模型組按100μg/kg皮下注射生理鹽水(NS)。另設(shè)假手術(shù)組(N=8)。2周后，超聲心動圖(UCG)檢測大鼠心功能，ELISA法檢測血清LDH、CK-MB含量，HE染色觀察心肌組織形態(tài)學(xué)變化，Western blotting檢測內(nèi)質(zhì)網(wǎng)應(yīng)激(ERS)標(biāo)志物GRP-78、CHOP蛋白表達(dá)。 結(jié)果成功建立AMI大鼠模型。與假手術(shù)組比較，UCG提示模型組大鼠心功能明顯下降(P 0.05)，HE染色可見組織疏松、水腫較明顯，血清LDH、CK-MB明顯升高(P 0.05)，Western blotting檢測顯示GRP-78、CHOP蛋白表達(dá)增加(P0.05)；與模型組比較，處理組心功能明顯改善，組織疏松、水腫明顯減輕，血清LDH、CK-MB降低(P0.05)，GRP-78、CHOP蛋白表達(dá)下降(P0.05)。 結(jié)論Ghrelin可能通過抑制ERS途徑改善AMI引起的心肌損傷，從而改善心功能。
[Abstract]:Objective to investigate the effect of Ghrelin on cardiac function in acute myocardial infarction (AMI) rats and its possible mechanism. Methods AMI model was established in 18 adult male SD rats by ligation of anterior descending coronary artery. On the 4th day after modeling, 14 surviving rats were randomly divided into two groups: the model group (n = 14) and the treatment group (n = 10). The treatment group was subcutaneously injected with ghrelin (100 渭 g/kg) twice a day for 12 weeks, and the model group was subcutaneously injected with 100 渭 g/kg normal saline. After 8.2 weeks in sham-operation group, the cardiac function of rats was detected by echocardiography (UCGG). The content of serum LDH- CK-MB was detected by Elisa. The morphological changes of myocardium were observed by HE staining. The expression of GRP-78 / CHOP was detected by Western blotting. Results AMI rat model was successfully established. Compared with the sham-operated group, UCG showed that the cardiac function of the model group was obviously decreased P 0.05 and HE staining showed that the tissue was loose, the edema was obvious, and the serum LDH- CK-MB was significantly higher than that of the sham operation group. Western blotting analysis showed that the expression of GRP-78 Chop protein was increased in the model group, and compared with the model group, the expression of GRP-78 Chop protein was increased in the model group. In the treatment group, the cardiac function was improved, the tissue was loose and edema was alleviated, and the expression of GRP-78 chop protein was decreased in serum LDH- CK-MB. Conclusion Ghrelin may improve cardiac function by inhibiting ERS pathway to improve myocardial injury induced by AMI.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R542.22

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