本文選題：顱腦損傷 + 神經(jīng)膠質(zhì)原纖維酸性蛋白質(zhì)��； 參考：《重慶醫(yī)學(xué)》2017年18期

【摘要】：目的探討大鼠顱腦損傷(TBI)后星形膠質(zhì)細胞(AST)瞬時受體電位通道(TRPC)6在TBI中扮演的角色。方法將健康成年雄性SD大鼠39只分為假手術(shù)組、致傷組和去鐵胺(DFX)組,每組13只。參照Feeney法建立大鼠大腦沖擊傷模型,完成Morris水迷宮實驗、大腦缺損體積,免疫熒光檢測TRPC6與神經(jīng)膠質(zhì)原纖維酸性蛋白質(zhì)(GFAP)共表達,以及Western blot定量檢測TRPC6水平。結(jié)果 DFX組比致傷組大鼠大腦缺損體積明顯減小[(115.35±13.70)mm3 vs.(209.99±16.70)mm3,P0.05],Morris水迷宮實驗發(fā)現(xiàn)DFX組平臺搜索策略[(3.13±0.35)分]和搜索時間[(36.15±26.63)s]均較致傷組[(2.13±0.64)分和(110±47.34)s]明顯改善(P0.05)。免疫熒光雙標發(fā)現(xiàn)DFX組GFAP高表達,且與TRPC6共表達增多。Western blot定量檢測發(fā)現(xiàn)DFX組TRPC6明顯下調(diào)(P0.01)。結(jié)論大鼠TBI早期DFX治療后AST活化,TRPC6高表達,進而起到神經(jīng)保護作用。
[Abstract]:Objective to investigate the role of TRPC-6 in TBI after brain injury (TBI) in rats. Methods 39 healthy adult male SD rats were divided into sham operation group, injury group and DFX group with 13 rats in each group. The rat brain blast injury model was established by Feeney method, the Morris water maze test was completed, the brain defect volume, the co-expression of TRPC6 and glial fibrillary acidic protein (GFAP) were detected by immunofluorescence, and the TRPC6 level was quantitatively detected by Western blot. Results compared with the injured rats, the volume of brain defect in DFX group was significantly reduced [115.35 鹵13.70)mm3 vs.(209.99 鹵16.70 mm / 3P] and Morris water maze test showed that the platform search strategy [3.13 鹵0.35] and the search time [36.15 鹵26.63 vs 2.13 鹵0.64] and 110 鹵47.34s in the DFX group were significantly improved than those in the injured group [2.13 鹵0.64 and 110 鹵47.34s]. The expression of GFAP was found to be high in DFX group with double immunofluorescence labeling, and the co-expression was increased with TRPC6. Western blot quantitative analysis showed that TRPC6 in DFX group was significantly down-regulated (P 0.01). Conclusion the high expression of AST activated TRPC6 after early DFX treatment in rats with TBI may play a neuroprotective role.
【作者單位】： 重慶市急救醫(yī)療中心神經(jīng)外科;第三軍醫(yī)大學(xué)西南醫(yī)院神經(jīng)外科;
【基金】：國家自然科學(xué)基金青年基金項目(30901545) 重慶市自然科學(xué)基金項目(cstc2015jcyjA1051) 重慶市衛(wèi)計委臨床重點�？祈椖�(渝衛(wèi)醫(yī)函2015-517)
【分類號】：R651.15

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