本文選題：單純性腦震蕩　切入點：AH　出處：《神經解剖學雜志》2016年05期 　論文類型：期刊論文

【摘要】：目的:觀察單純性腦震蕩(pure cerebral concussion,PCC)致傷后大鼠海馬膜結合型前列腺素E2-合酶1(membrane-associated prostaglandin E synthase-1,mPGES-1)在EP2受體抑制劑(AH6809)干預后的表達變化,探討AH6809對mPGES-1的干預調控作用。方法:采用金屬單擺閉合式腦損傷打擊裝置制備PCC模型,分別于致傷后即刻和12 h腹腔注射AH6809,然后隨機分為1、2、3 d和7 d組(n=5),另設相應生理鹽水對照組(n=5)。采用免疫組織化學染色和Western Blot方法,觀察PCC+AH6809組和生理鹽水對照組大鼠海馬(CA1~4和上、下齒狀回)內m PGES-1的表達情況。結果:免疫組化實驗結果顯示:在AH6809干預前后均可觀察到mPGES-1陽性產物表達于海馬CA1~4和上、下齒狀回的神經元胞體,呈圓形或卵圓形,胞核不著色,胞漿為棕黃色。與生理鹽水對照組相比,AH6809干預后2 d組均出現(xiàn)mPGES-1陽性表達相對減弱,染色漸淺且細胞輪廓不清,3 d組下調最為明顯,其中3 d組和7 d組的差異具有顯著性(P0.05)。Western Blot實驗結果顯示:mPGES-1蛋白出現(xiàn)相似的規(guī)律性變化,下降的低峰出現(xiàn)在7 d組,其中2、3、7 d組與對照組相比差異具有顯著性(P0.05)。結論:AH6809對PCC大鼠海馬mPGES-1的表達具有一定的干預抑制作用。
[Abstract]:Aim: to observe the expression of membrane-associated prostaglandin E2-synthase (PGES-1) in the hippocampus of rats after injury induced by pure cerebral concussion.Methods: the expression of membrane-associated prostaglandin E synthase-1mPGES-1 (mPGES-1) in the hippocampus of rats after injury induced by pure cerebral concussion-PCC-1 was observed in the presence of EP2 receptor inhibitor AH6809. To investigate the effect of AH6809 on the intervention and regulation of mPGES-1. Methods: the model of PCC was established by metal pendulum closed brain injury device. AH6809 was injected intraperitoneally immediately and 12 hours after injury, and then randomly divided into 2 groups (n = 3) and 7 days (n = 7). The corresponding saline control group (n = 5) was treated with immunohistochemical staining and Western Blot method. The expression of mPGES-1 in hippocampus of PCC AH6809 group and normal saline control group was observed. Results: before and after AH6809 intervention, the positive expression of mPGES-1 was observed in CA1~4 and upper dentate gyrus of hippocampus. The neuronal bodies of the inferior dentate gyrus were round or oval, the nuclei were not stained, and the cytoplasm was brown. Compared with the normal saline control group, the positive expression of mPGES-1 was decreased relatively in the control group 2 days after intervention. The down-regulation was most obvious in the groups with light staining and unclear cell profile. The difference between the groups of 3 days and 7 days was significant (P 0.05). Western Blot results showed that the protein of 1% mPGES-1 showed similar regular changes, and the low peak of the decrease appeared in the group of 7 days. There was significant difference between the control group and the control group on the 7th day. Conclusion: AH6809 can interfere with the expression of mPGES-1 in hippocampus of PCC rats.
【作者單位】： 昆明醫(yī)科大學;昆明醫(yī)科大學人體解剖學與組織胚胎學系;昆明醫(yī)科大學法醫(yī)學院;昆明醫(yī)科大學腦損傷研究室;
【基金】：國家自然科學基金(31100769,30560041) 云南省自然科學基金(2008CD052)
【分類號】：R651.15

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