本文關(guān)鍵詞： 百草枯 中毒 新斯的明 肺損傷　出處：《第三軍醫(yī)大學(xué)學(xué)報(bào)》2017年21期 　論文類型：期刊論文

【摘要】：目的觀察膽堿酯酶抑制劑——新斯的明對百草枯中毒大鼠全身炎癥反應(yīng)和肺損傷的影響。方法 96只SD大鼠隨機(jī)分為3組(n=32)。NES組:腹腔注射百草枯(paraquat,PQ)溶液20 mg/kg后2 h腹腔注射新斯的明300μg/kg;PQ組:腹腔注射等量PQ溶液后2 h腹腔注射生理鹽水300μg/kg;對照組:以等量生理鹽水代替PQ溶液腹腔注射。在6、24、72 h 3個(gè)時(shí)間點(diǎn)觀察大鼠血清及肺組織TNF-α、IL-6、IL-10變化;分批處死大鼠進(jìn)行肺組織病理學(xué)觀察以及測量肺濕/干質(zhì)量(W/D);Real-Time PCR檢測肺組織SOCS1、SOCS3 mRNA表達(dá)。結(jié)果 PQ染毒后,大鼠血清和肺組織TNF-α、IL-6、IL-10均顯著升高,NES組在染毒后各時(shí)間點(diǎn)血清和肺組織TNF-α水平低于PQ組(P0.05),在染毒后24、72 h血清及肺組織IL-6水平低于PQ組(P0.05)。染毒后各時(shí)間點(diǎn)NES組IL-10水平高于PQ組(P0.05)。染毒后72 h,病理學(xué)觀察可見NES組大鼠肺損傷程度較PQ組輕。NES組24、72 h肺濕干質(zhì)量比低于PQ組(P0.05)。PQ染毒后各時(shí)間點(diǎn)SOCS1、SOCS3 mRNA表達(dá)水平高于對照組(P0.05)。NES組SOCS3 mRNA表達(dá)水平高于PQ組,但SOCS1 mRNA表達(dá)水平與PQ組差異無統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論新斯的明能減輕PQ中毒大鼠的全身炎癥反應(yīng)和肺損傷程度,其機(jī)制可能涉及激活膽堿能抗炎途徑
[Abstract]:Objective to observe the effects of cholinesterase inhibitor neostigmine on systemic inflammation and lung injury in paraquat poisoned rats. Methods 96 SD rats were randomly divided into 3 groups (n = 3). NES group: paraquat of paraquat was injected intraperitoneally. Two hours after 20 mg/kg of PQ solution, neostigmine was injected intraperitoneally with neostigmine (300 渭 g / kg). In PQ group, 300 渭 g / kg saline was injected intraperitoneally 2 hours after intraperitoneal injection of the same amount of PQ solution. In the control group, the rats were injected intraperitoneally with the same amount of normal saline instead of PQ solution. The changes of IL-10 in serum and lung tissue of rats were observed at 6: 2472 h. The rats were sacrificed in batches to observe the lung histopathology and to measure the wet / dry weight of lung. Real-Time PCR was used to detect the expression of SOCS1 and SOCS3 mRNA in lung tissue. Results after PQ exposure, TNF- 偽 IL-6 was detected in serum and lung tissue of rats. The levels of TNF- 偽 in serum and lung tissue in IL-10 group were significantly higher than those in PQ group at all time points after exposure, and 24 hours after exposure. The level of IL-6 in serum and lung tissue was lower than that in PQ group at 72 h, and the IL-10 level in NES group was higher than that in PQ group at each time point after exposure to PQ, and at 72 h after exposure, the level of IL-10 in NES group was higher than that in PQ group. Pathological observation showed that the degree of lung injury in NES group was lighter than that in PQ group. The ratio of wet and dry lung weight in NES group was lower than that in PQ group at 2472 hours. The expression level of SOCS3 mRNA was higher than that of the control group (P0.05N. NES) and the expression of SOCS3 mRNA was higher than that of PQ group. However, the expression of SOCS1 mRNA was not significantly different from that of PQ group. Conclusion neostigmine can alleviate systemic inflammation and lung injury in PQ poisoned rats. Its mechanism may involve activating cholinergic anti-inflammatory pathway.
【作者單位】： 重慶市急救醫(yī)療中心急診科;重慶市急救醫(yī)療中心ICU;
【基金】：重慶市衛(wèi)生計(jì)生委醫(yī)學(xué)科研項(xiàng)目:衛(wèi)計(jì)委面上項(xiàng)目(20142082)~~
【分類號】：R595.4
【正文快照】： 心:急診科1,ICU2)百草枯(paraquat,PQ)是廣泛應(yīng)用于發(fā)展中國家農(nóng)村的一種除草劑,誤食、服毒往往引起嚴(yán)重的藥物中毒,大多數(shù)中毒患者由消化道吸收。該病具有發(fā)病時(shí)間早、進(jìn)展快、致死量低、病死率高的特點(diǎn),患者常因嚴(yán)重的全身炎癥反應(yīng)以及不可逆的肺纖維化和全身多臟器功能衰竭

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 馮蕊珍;肌注新斯的明過敏報(bào)道[J];山西護(hù)理雜志;1988年04期

2 譚開云;新斯的明致休克1例[J];現(xiàn)代應(yīng)用藥學(xué);1997年06期

3 ;肌注新斯的明引起舌體強(qiáng)直、言語不便一例報(bào)告[J];天津醫(yī)藥;1976年02期

4 尹宗信;持續(xù)皮下注入新斯的明對嚴(yán)重重癥肌無力病人的處理[J];國外醫(yī)學(xué)(內(nèi)科學(xué)分冊);1980年Z1期

5 孟愛宏;王保法;袁雅冬;潘文森;閻錫新;;急性藥物性肺損傷臨床分析:附典型病例[J];中國全科醫(yī)學(xué);2009年20期

6 徐欽星;任振義;王利民;;藥物性肺損傷三例[J];中華臨床醫(yī)師雜志(電子版);2012年02期

7 ;來稿簡報(bào)[J];實(shí)用內(nèi)科雜志;1983年04期

8 施舉紅;嚴(yán)曉偉;許文兵;劉鴻瑞;朱元玨;;藥物性肺損傷的臨床診斷與治療[J];中華結(jié)核和呼吸雜志;2007年03期

9 于春秋,孫蘭芬;新斯的明搶救河，

本文編號：1488608