本文關(guān)鍵詞： 急性心肌梗死(AMI) 生長分化因子-15(GDF-15) 基因多態(tài)性 聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性(PCR-RFLP) 側(cè)枝循環(huán)　出處：《山西醫(yī)科大學(xué)》2013年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：探討生長分化因子-15(GDF-15)基因-3148C／G位點(diǎn)多態(tài)性與太原地區(qū)漢族人群急性心肌梗死(AMI)側(cè)枝循環(huán)形成的關(guān)系。 方法：收集92例急性心肌梗死組(血管急性閉塞3-12小時內(nèi))患者作為病例組(有側(cè)枝組68例,無側(cè)枝組24例)和56例冠造正常者作為對照組,兩組樣本均取自太原地區(qū)漢族人群。提取外周血DNA,運(yùn)用聚合酶鏈反應(yīng)-限制性片段長度多態(tài)性(PCR-RFLP)、DNA測序及序列比對的方法分析GDF-15基因-3148C／G位點(diǎn)多態(tài)性與急性心肌梗死側(cè)枝循環(huán)形成的相關(guān)性。 結(jié)果：太原地區(qū)漢族人群中GDF-15基因-3148C／G位點(diǎn)經(jīng)檢測存在兩種基因型：CC和GC基因型,而未檢測到GG基因型。急性心肌梗死組和正常對照組人群均符合Hardy-Weinberg遺傳平衡定律,具有群體代表性。在急性心肌梗死組中,CC、GC兩種基因型頻率分別為80.43%和19.57%,在正常對照組中分別為60.71%和39.29%；兩組受試者-3148C／G位點(diǎn)的CC、GC基因型頻率分布有統(tǒng)計學(xué)意義(X2=6.864,P=0.009)；風(fēng)險基因型為GC型,與基因型CC型相比,攜帶GC基因型可使發(fā)生急性心肌梗死的風(fēng)險升高2.6倍(OR=2.660,95%可信區(qū)間為1.265-5.595)。在急性心肌梗死患者血管急性閉塞3-12小時內(nèi)有側(cè)枝形成組,CC、GC基因型頻率分別為85.29%和14.71%,在無側(cè)枝形成組中,CC、GC基因型頻率分別分別為66.67%和33.33%；兩組受試者-3148C／G位點(diǎn)的CC、GC基因型頻率分布有統(tǒng)計學(xué)意義(X2=3.911,P=0.048)；風(fēng)險基因型為GC基因型,與基因型CC型相比,攜帶GC基因型的急性心肌梗死患者,其血管急性閉塞3-12小時內(nèi)側(cè)枝循環(huán)形成的可能性較基因型CC型增高2.9倍(0R=2.900,95%可信區(qū)間為0.983-8.556)。 結(jié)論：GDF-15基因-3148C／G位點(diǎn)中,GC基因型攜帶者發(fā)生急性心肌梗死的易感性大于CC基因型攜帶者,同時,在急性心肌梗死患者血管閉塞3-12小時內(nèi),攜帶GC基因型者更易于側(cè)枝循環(huán)的形成,檢測GDF-15的基因型可作為急性心肌梗死早期診斷和防治的重要標(biāo)記物。
[Abstract]:Objective: to investigate the relationship between the polymorphism of growth differentiation factor-15 (GDF-15) gene -3148C / G locus and collateral circulation formation in acute myocardial infarction (AMI) of Han nationality in Taiyuan. Methods: 92 patients with acute myocardial infarction (within 3-12 hours of acute vascular occlusion) were selected as the case group (68 cases with collateral branch group, 24 cases with no collateral branch group) and 56 cases with normal coronary artery as control group. The samples of both groups were extracted from the Han population in Taiyuan area. The peripheral blood DNA was extracted and PCR-RFLP was used to detect the DNA in the peripheral blood by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The relationship between the polymorphism of GDF-15 gene -3148C / G locus and collateral circulation in acute myocardial infarction was analyzed by DNA sequencing and sequence alignment. Results: there were two genotypes of GDF-15 gene in Taiyuan Han population: -3148C / G locus. There were two genotypes: 1. CC and GC. But no GG genotypes were detected. Both acute myocardial infarction (AMI) group and normal control group were in accordance with the law of genetic balance of Hardy-Weinberg. The frequencies of the two genotypes were 80.43% and 19.57, respectively, and 60.71% and 39.29 in the normal control group, respectively. The frequency distribution of CCG genotype at -3148C / G locus in the two groups was statistically significant (P < 0.05). Compared with CC genotype, carrying GC genotype increased the risk of acute myocardial infarction by 2.6 times. The confidence interval of 95% was 1.265-5.595 渭 m. In acute vascular occlusion patients with acute myocardial infarction within 3-12 hours, there were lateral branch forming group (CC). The frequency of GC genotype was 85.29% and 14.71, respectively. In the group without lateral branch formation, the frequency of GC genotype was 66.67% and 33.33, respectively. The frequency distribution of CCG genotype at -3148C / G locus in the two groups was statistically significant (X _ 2N _ (3.911) P ~ (0.048)); The risk genotype was GC genotype, and compared with CC genotype, acute myocardial infarction patients with GC genotype. The probability of collateral circulation formation was 2.9 times higher than that of CC genotype within 3-12 hours after acute occlusion. The 95% confidence interval of 0.983-8.556 was 0.983-8.556. Conclusion the susceptibility of GC genotype carriers to acute myocardial infarction is higher than that of CC genotype carriers. Patients with GC genotype were more likely to form collateral circulation within 3-12 hours of occlusion in patients with acute myocardial infarction. Detection of GDF-15 genotypes may be an important marker for early diagnosis and prevention of acute myocardial infarction.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R542.22

【共引文獻(xiàn)】

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本文編號：1484898