本文關(guān)鍵詞： 干細(xì)胞 神經(jīng)干細(xì)胞 組織工程 分化 內(nèi)源性神經(jīng)干細(xì)胞因子 脈沖磁場 顱腦損傷 大鼠 腦功能　出處：《中國組織工程研究》2017年21期 　論文類型：期刊論文

【摘要】：背景:有研究指出,脈沖電磁在促進(jìn)及誘導(dǎo)腦梗死、脊髓損傷周圍神經(jīng)再生,改善神經(jīng)疾病患者記憶功能等方面有良好效果。目的:探討脈沖磁場對(duì)顱腦損傷大鼠腦功能及腦組織中內(nèi)源性神經(jīng)干細(xì)胞因子的影響。方法:將320只雄性成年SD大鼠隨機(jī)分為模型組、脈沖磁場0.1 mT組、脈沖磁場0.3 mT組及脈沖磁場0.5 mT組,均采用側(cè)向液壓打擊法建立顱腦損傷模型,脈沖磁場0.1 mT組、脈沖磁場0.3 mT組及脈沖磁場0.5 mT組建模后給予相應(yīng)強(qiáng)度的脈沖磁場照射,照射后1,3,7,14 d,進(jìn)行平衡木行走實(shí)驗(yàn)、水迷宮實(shí)驗(yàn),以測定大鼠運(yùn)動(dòng)功能;相應(yīng)照射時(shí)間點(diǎn)前1 d,腹腔注射BrdU,應(yīng)用免疫組織化學(xué)法測定腦組織BrdU及皮質(zhì)巢蛋白陽性細(xì)胞數(shù)。結(jié)果與結(jié)論:(1)照射后1,3,7 d,脈沖磁場0.1,0.3,0.5 mT組水迷宮實(shí)驗(yàn)時(shí)間、平衡木行走實(shí)驗(yàn)均短于模型組(P0.05),脈沖磁場0.5 mT組水迷宮實(shí)驗(yàn)時(shí)間、平衡木行走實(shí)驗(yàn)均短于脈沖磁場0.1,0.3 mT組(P0.05),脈沖磁場0.3 mT組水迷宮實(shí)驗(yàn)時(shí)間、平衡木行走實(shí)驗(yàn)均短于脈沖磁場0.1 mT組(P0.05);(2)照射后3,7,14 d,脈沖磁場0.1,0.3,0.5 mT組Brd U及皮質(zhì)巢蛋白陽性細(xì)胞數(shù)多于模型組(P0.05),脈沖磁場0.5 mT組Brd U及皮質(zhì)巢蛋白陽性細(xì)胞數(shù)多于脈沖磁場0.1,0.3 mT組(P0.05),脈沖磁場0.3 mT組Brd U及皮質(zhì)巢蛋白陽性細(xì)胞數(shù)多于脈沖磁場0.1 mT組(P0.05);(3)結(jié)果表明,脈沖磁場能明顯保護(hù)顱腦損傷大鼠腦功能,且電磁場強(qiáng)度越強(qiáng)效果越明顯,其可能機(jī)制與脈沖磁場激活顱腦損傷大鼠腦組織中神經(jīng)干細(xì)胞及促進(jìn)其增殖有關(guān)。
[Abstract]:Background: it has been pointed out that pulse electromagnetism promotes and induces the regeneration of peripheral nerve after cerebral infarction and spinal cord injury. Objective: to investigate the effect of pulsed magnetic field on brain function and endogenous neural stem cell factor in brain tissue of rats with craniocerebral injury. 320 adult male SD rats were randomly divided into model group. The craniocerebral injury model was established by lateral hydraulic beating method in the pulse magnetic field 0.1 Mt group, the pulse magnetic field 0.3 Mt group and the pulse magnetic field 0.5 Mt group, and the pulse magnetic field 0.1 Mt group. The pulse magnetic field 0.3 Mt group and the pulse magnetic field 0.5 Mt group were treated with the corresponding intensity of pulsed magnetic field irradiation. The balance beam walking experiment and the water maze experiment were carried out on the first day after irradiation. To determine the motor function of rats; BrdU was injected intraperitoneally 1 day before the corresponding irradiation time point. The number of BrdU and cortex nestin positive cells in brain tissue was determined by immunohistochemical method. After 7 days, the experimental time of water maze and the walking experiment of balance beam in 0.1mt 0.3mT group were shorter than those in model group (P0.05). The experimental time of water maze and walking test of balance beam in 0.5 Mt group were shorter than that in 0.3 Mt group (P 0.05). The experimental time of water maze and walking test of balance beam in 0.3 Mt group were shorter than that in 0.1 Mt group. (2) the number of Brd U and nestin positive cells in 0. 1 ~ 0. 3mT group was more than that in the model group (P 0. 05). The number of Brd U and nestin positive cells in pulsed magnetic field group was more than that in 0.1mT group (P 0.05). The number of Brd U and nestin positive cells in the pulse magnetic field group was more than that in the pulse magnetic field group. The results show that pulsed magnetic field can obviously protect brain function of rats with brain injury, and the stronger the electric magnetic field is, the more obvious the effect is. The mechanism may be related to the activation and proliferation of neural stem cells in brain tissue after brain injury by pulsed magnetic field.
【作者單位】： 鄭州大學(xué)附屬醫(yī)院南陽市中心醫(yī)院創(chuàng)傷外科;鄭州大學(xué)附屬醫(yī)院南陽市中心醫(yī)院神經(jīng)外科;鄭州大學(xué)附屬醫(yī)院南陽市中心醫(yī)院臨床藥學(xué)科;鄭州大學(xué)第一附屬醫(yī)院;
【分類號(hào)】：R651.15
【正文快照】： 文章快速閱讀:脈沖磁場促進(jìn)顱腦損傷大鼠腦組織中內(nèi)源性神經(jīng)干細(xì)胞的增殖結(jié)果:分組干預(yù):檢測指標(biāo):(1)脈沖磁場能明顯保護(hù)(1)顱腦損傷模型;(1)照射后1,3,7,14 d,進(jìn)顱腦損傷大鼠腦功能;(2)顱腦損傷模型+脈行平衡木行走實(shí)驗(yàn)、水迷宮實(shí)(2)且電磁場強(qiáng)度越強(qiáng)效沖磁場0.1 mT干預(yù);驗(yàn),

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Fabio Guerriero;Giovanni Ricevuti;;Extremely low frequency electromagnetic fields stimulation modulates autoimmunity and immune responses:a possible immuno-modulatory therapeutic effect in neurodegenerative diseases[J];Neural Regeneration Research;2016年12期

2 黃佳;鄭薏;姚建寧;葉曉倩;王露露;陶靜;柳維林;;MicroRNA-9調(diào)控室管膜下區(qū)干細(xì)胞增殖在電針治療局灶性腦缺血中的作用[J];中國康復(fù)理論與實(shí)踐;2016年11期

3 楊永凱;張帆;陳春美;王春華;韋浩;周曉輝;劉春金;;高壓氧對(duì)大鼠顱腦損傷后海馬區(qū)神經(jīng)干細(xì)胞增殖分化的影響[J];中華實(shí)驗(yàn)外科雜志;2016年11期

4 Li-juan Ye;Hui Bian;Yao-dong Fan;Zheng-bo Wang;Hua-lin Yu;Yuan-ye Ma;Feng Chen;;Rhesus monkey neural stem cell transplantation promotes neural regeneration in rats with hippocampal lesions[J];Neural Regeneration Research;2016年09期

5 Yue Yao;Xiang-rong Zheng;Shan-shan Zhang;Xia Wang;Xiao-he Yu;Jie-lu Tan;Yu-jia Yang;;Transplantation of vascular endothelial growth factor-modified neural stem/progenitor cells promotes the recovery of neurological function following hypoxic-ischemic brain damage[J];Neural Regeneration Research;2016年09期

6 隋立森;余佳彬;姜曉丹;;大鼠創(chuàng)傷性顱腦損傷后腦室下區(qū)內(nèi)源性神經(jīng)干細(xì)胞的增殖與分化[J];南方醫(yī)科大學(xué)學(xué)報(bào);2016年08期

7 李春偉;伊志強(qiáng);李良;;重型創(chuàng)傷性顱腦損傷的治療進(jìn)展[J];中國微創(chuàng)外科雜志;2016年07期

8 Hai-xiao Zhou;Zhi-gang Liu;Xiao-jiao Liu;Qian-xue Chen;;Umbilical cord-derived mesenchymal stem cell transplantation combined with hyperbaric oxygen treatment for repair of traumatic brain injury[J];Neural Regeneration Research;2016年01期

9 趙明亮;陳以勝;李曉紅;王景景;涂悅;孫洪濤;張賽;陳，

本文編號(hào)：1477728