本文關(guān)鍵詞：大麻素受體2對(duì)重癥急性胰腺炎的影響　出處：《蘭州大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 大麻素受體2 重癥急性胰腺炎 肺損傷 肝損傷、腎損傷

【摘要】：目的：主要探討大麻素受體2(Cannabinoid receptor2, CB2)對(duì)重癥急性胰腺炎(Severe acute pancreatitis, SAP)導(dǎo)致的肝、腎、肺損傷的作用。 方法：雄性SPF級(jí)SD大鼠96只按隨機(jī)數(shù)字表法分成4組,對(duì)照組：僅開(kāi)腹后翻動(dòng)胰腺組織后關(guān)腹；SAP組：4%�；悄懰徕c逆行注入胰管建立SAP模型；HU-308組：腹腔注射HU-3082.5mg/kg,1h后建立SAP模型；AM-630組：腹腔注射AM-6302.5mg/kg,1h后建立SAP模型。分別在3h、6h、12h分離血清低溫保存,檢測(cè)血清中淀粉酶(Amylase, AMY),腫瘤壞死因子-α (Tumor necrosis factor-α, TNF-α)、白介素-6(Interleukin-6, IL-6)、谷丙轉(zhuǎn)氨酶(Alanine aminotransferase, ALT)、谷草轉(zhuǎn)氨酶(Aspartate Transaminase, AST)、血尿素氮(Blood urea nitrogen, BUN)、肌酐(Creatinine, Cr)水平。取新鮮左肺下葉勻漿后檢測(cè)髓過(guò)氧化物酶(Myeloperoxidase, MPO)水平,將右肺下葉制作成HE病理切片并進(jìn)行病理學(xué)評(píng)分。其余肺組織稱重后進(jìn)行干燥處理,測(cè)量濕干重之比。 結(jié)果：在每個(gè)時(shí)間點(diǎn),SAP組、AM-630組、HU-308組中AMY、TNF-α、 IL-6、MPO、肺組織濕干重比值和病理學(xué)評(píng)分均高于對(duì)照組(P0.05);在3h時(shí)間點(diǎn),SAP組、AM-630組、HU-308組中ALT、AST、BUN、Cr水平分別與對(duì)照組相比較,無(wú)明顯差異(P0.05),在6h、12h時(shí)間點(diǎn)SAP組、AM-630組、HU-308組中ALT、AST、BUN、Cr水平均高于對(duì)照組(P0.05)。在3h時(shí)間點(diǎn),HU-308組中AMY、TNF-α、IL-6、MPO、肺組織濕干重比值、病理學(xué)評(píng)分均低于SAP組(P0.05),ALT、AST、BUN、Cr水平與SAP組無(wú)明顯差異(P0.05)；在6h、12h時(shí)間點(diǎn),HU-308組中AMY、TNF-α、IL-6、MPO、肺組織濕干重比值、病理學(xué)評(píng)分、ALT、AST、BUN和Cr水平均低于SAP組(P0.05)。在3h時(shí)間點(diǎn),AM-630組中AMY、TNF-α、IL-6和MPO水平高于SAP組(P0.05),肺組織濕干重比值、病理學(xué)評(píng)分、ALT、AST、BUN和Cr水平與SAP組相比較,無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)；在6h時(shí)間點(diǎn),AM-630組中AMY、TNF-α、IL-6、MPO、肺組織濕干重比值、病理學(xué)評(píng)分、AST、BUN和Cr水平高于SAP組(P0.05), ALT水平與SAP組相比較無(wú)統(tǒng)計(jì)學(xué)差異(P0.05)；在12h時(shí)間點(diǎn),AM-630組中所檢測(cè)的所有指標(biāo)均高于SAP組(P0.05)。 結(jié)論：CB2受體的激活對(duì)SAP導(dǎo)致的肝、腎、肺損傷起到保護(hù)作用,CB2受體被抑制后肝、腎、肺損傷的程度加重。
[Abstract]:Objective: to investigate cannabinoid receptor2 of cannabinoid receptor 2. The effect of CB2 on liver, kidney and lung injury induced by severe acute pancreatitis (SAP). Methods: 96 male SPF SD rats were randomly divided into 4 groups. SAP model was established by retrograde injection of sodium taurocholate into pancreatic duct in SAP group. In HU-308 group, SAP model was established after intraperitoneal injection of HU-3082.5 mg / kg for 1 hour. In AM-630 group, the SAP model was established after intraperitoneal injection of AM-6302.5mg / kg for 1 h. Serum amylase Amylase, amylase, tumor necrosis factor- 偽 Tumor necrosis factor- 偽 (TNF- 偽) were detected. Interleukin-6, IL-6, alanine aminotransferase (alt). Aspartate transaminase (AST), blood urea nitrogenin (bunn). The levels of myeloperoxidase (MPO) were measured after fresh left lower lung homogenate. The lower lobe of the right lung was made into HE pathological sections and the other lung tissues were dried after weighing and the ratio of wet and dry weight was measured. Results: AMYTNF- 偽 and IL-6 MPO were detected in AM-630 group and HU-308 group at each time point. The wet / dry weight ratio and pathological score of lung tissue were higher than that of control group (P 0.05). At 3 h, there was no significant difference in the levels of alt ASTT bun Cr between the SAP group and the control group (P 0. 05) in AM-630 group and HU-308 group (P < 0. 05, P < 0. 05, P 0. 05, P 0. 05, P < 0. 05). At the time point of 6 h and 12 h, the levels of alt ASTT bun Cr in the SAP group were higher than those in the control group (P 0.05), and at the 3h time point, the levels of alt ASTT bun Cr in the HU-308 group were higher than those in the control group (P < 0.05). In HU-308 group, AMYT TNF- 偽 IL-6 MPO, lung wet / dry weight ratio and pathological score were lower than those in SAP group (P 0.05). There was no significant difference between Cr level and SAP group (P 0.05). In HU-308 group, AMYT TNF- 偽 IL-6 MPO, lung wet / dry weight ratio, pathological score and AST were measured at 6 h and 12 h. The levels of BUN and Cr in AM-630 group were lower than those in SAP group (P 0.05). The levels of IL-6 and MPO were higher than that of SAP group (P 0.05), the ratio of wet and dry weight of lung tissue, and the pathological scores of alt ASTT bun and Cr were compared with those of SAP group. There was no statistical difference (P 0.05). AM-630 group was treated with AMYT TNF- 偽 IL-6 MPO, lung wet / dry weight ratio, pathological score and AST. The levels of BUN and Cr in SAP group were higher than those in SAP group (P 0.05), and there was no significant difference in ALT level between SAP group and ALT group (P 0.05). All the indexes detected in AM-630 group at 12h were higher than those in SAP group (P 0.05). Conclusion the activation of the CB2 receptor can protect the liver, kidney and lung injury induced by SAP. The degree of liver, kidney and lung injury after the inhibition of CB2 receptor is aggravated.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R657.51

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