【摘要】：目的對(duì)2014年廣州與佛山地區(qū)登革病毒C-prM區(qū)進(jìn)行基因測(cè)序,從分子水平與相關(guān)國(guó)際流行株進(jìn)行同源性比較,構(gòu)建系統(tǒng)發(fā)育樹(shù),追蹤其可能輸入來(lái)源。方法收集本院2014年9月-11月447例登革熱疑似患者急性期血清,隨機(jī)選取其中廣州與佛山地區(qū)不同區(qū)域的24例標(biāo)本,提取病毒RNA,RT-PCR法擴(kuò)增C-prM基因,并將其克隆到p MD19T載體,測(cè)序并利用生物信息學(xué)軟件進(jìn)行分析。結(jié)果成功測(cè)序登革病毒11株,BLAST分析表明均為登革1型病毒(DENV-1)。系統(tǒng)發(fā)育樹(shù)結(jié)果顯示GZ2014-01等序列與2010年印度德里流行株同源性高(相似度為99.21%),GZ2014-02等序列與2013年廣州流行株相近(相似度為97.64%~98.82%)。廣州株GZ2014-01等和佛山株GZ2014-23屬于基因型Ⅴ,而廣州株GZ2014-02等和佛山株GZ2014-20屬于基因型Ⅰ。結(jié)論 GZ2014-01等株可能來(lái)源于印度德里,而GZ2014-02等株可能來(lái)源于中國(guó)廣州。登革病毒多基因型流行可能是造成2014年登革熱大暴發(fā)的原因之一。
[Abstract]:Aim to sequence the C-prM region of dengue virus in Guangzhou and Foshan in 2014 and compare it with the related international strains at molecular level in order to construct phylogenetic tree and trace its possible input source. Methods Serum samples were collected from 447 suspected dengue fever patients in our hospital from September to November, 2014. 24 samples from different regions of Guangzhou and Foshan were randomly selected and the C-prM gene was amplified by viral RNA,RT-PCR. It was cloned into p-MD19T vector, sequenced and analyzed by bioinformatics software. Results 11 strains of dengue virus were sequenced successfully. BLAST analysis showed that all strains were dengue type 1 virus (DENV-1). Phylogenetic tree analysis showed that GZ2014-01 et al had high homology with India Delhi epidemic strain in 2010 (similarity was 99.21%), and GZ2014-02 et al sequence was similar to Guangzhou epidemic strain in 2013 (97.64% and 98.82% similarity). Guangzhou strain GZ2014-01 et al and Foshan strain GZ2014-23 belong to genotype 鈪，

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