【摘要】：侵襲性曲霉病以煙曲霉在肺部定植、侵襲為主,具有較高的致死率.治療侵襲性曲霉病的關(guān)鍵是早期確診.目前臨床上用于診斷侵襲性曲霉病的血清學方法是檢測血清中半乳甘露聚糖循環(huán)抗原的GM試驗,在敏感性和特異性方面存在局限性.為了用抗重組蛋白多克隆抗體鑒定和分析煙曲霉Asp f3和Asp f4特異性和曲霉廣譜性表位,構(gòu)建檢測可疑血清樣本中相應抗體的表位嵌合肽,以診斷侵襲性曲霉病.本研究表達純化了煙曲霉Asp f3和Asp f4重組蛋白,制備了IgG抗體滴度均在1.28×106以上的高滴度的兔抗重組蛋白多克隆抗體.
[Abstract]:Invasive Aspergillus fumigatus was colonized in the lung, mainly invasive, with high mortality. The key to the treatment of invasive aspergillosis is early diagnosis. The present serological method for the diagnosis of invasive aspergillosis is the GM test for the detection of circulating antigen of galactomannan in serum, which is limited in sensitivity and specificity. In order to identify and analyze the specific and broad spectrum epitopes of Asp f3 and Asp f4 of Aspergillus fumigatus with polyclonal antibodies against recombinant proteins, an epitope chimeric peptide was constructed to detect the corresponding antibodies in suspicious serum samples for the diagnosis of invasive aspergillosis. In this study, recombinant proteins of Asp f3 and Asp f4 of Aspergillus fumigatus were expressed and purified, and rabbit anti-recombinant protein polyclonal antibodies with high titer of IgG antibody above 1.28 脳 10 ~ 6 were prepared.
【作者單位】： 復旦大學生命科學學院遺傳工程國家重點實驗室;上海市工業(yè)菌株工程技術(shù)研究中心;上海市計劃生育科學研究所國家人口和計劃生育委員會重點實驗室;
【基金】：國家重點基礎研究發(fā)展計劃(2013CB531603) 上海市科學技術(shù)委員會項目(13DZ2252000)
【分類號】：R519

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