艾滋病患者合并感染的分枝桿菌的菌種構(gòu)成與分子特征分析
發(fā)布時間：2018-08-24 07:21
【摘要】：目的獲得HIV/AIDS患者合并感染的分枝桿菌的菌種和構(gòu)成比。檢測HIV/AIDS患者合并感染的結(jié)核桿菌耐藥基因突變率,并對結(jié)核分枝桿菌進(jìn)行毒力的分析。方法通過分枝桿菌通用引物對細(xì)菌DNA保守序列hsp65進(jìn)行PCR擴(kuò)增并進(jìn)行測序；使用巢式PCR對HIV/AIDS患者結(jié)核菌inhA、rpoB、katG、embB、rpsL、rrs和pncA這7個一線抗結(jié)核藥物相關(guān)耐藥基因進(jìn)行擴(kuò)增并進(jìn)行測序,檢測相關(guān)耐藥基因突變率；通過RD105(regions of difference105)缺失鑒定技術(shù)來分析結(jié)核菌株中“北京型”的比例,由此來進(jìn)行結(jié)核分枝桿菌感染毒力的分析。 結(jié)果326例HIV/AIDS患者分離出的分枝桿菌DNA標(biāo)本中有陽性結(jié)果標(biāo)本數(shù)為244例,陰性結(jié)果標(biāo)本數(shù)為82例。陽性結(jié)果中結(jié)核桿菌167株,占68.4%；非結(jié)核分枝桿菌77株,占31.6%。非結(jié)核分枝桿菌包括戈登分枝桿菌39株,鳥-胞內(nèi)分枝桿菌復(fù)合群18株,堪薩斯分枝桿菌12株,膿腫分枝桿菌3株,偶遇分枝桿菌2株,馬賽分枝桿菌2株,蟾分枝桿菌1株。除inhA無突變以外,其余6個基因均有突變,rpoB、katG、embB、rpsL、rrs和pncA這6個基因的突變率分別為11.9%、7.7%、3.8%、12.7%、4.8%、6.1%。82株HIV/AIDS患者合并感染的結(jié)核桿菌中,“北京型”63株,占76.8%；“非北京型”19株,占23.1%。 結(jié)論分枝桿菌感染是HIV/AIDS患者常見的并發(fā)癥之一,其中結(jié)核分枝桿菌、鳥-胞內(nèi)分枝桿菌復(fù)合群和堪薩斯分枝桿菌又為HIV/AIDS患者分枝桿菌感染并發(fā)癥中最常見的四種菌種。HIV/AIDS患者結(jié)核菌的rpoB、katG、embB、rpsL、rrs和pncA這6個基因存在不同的突變率。“北京型”菌株是結(jié)核桿菌中的毒力較強(qiáng)菌株,而艾滋病患者分離出的結(jié)核桿菌中“北京型”占大多數(shù)。
[Abstract]:Objective to obtain the species and composition of Mycobacterium coinfection in patients with HIV/AIDS. The mutation rate of drug resistance gene of Mycobacterium tuberculosis co-infected with HIV/AIDS was detected and the virulence of Mycobacterium tuberculosis was analyzed. Methods the conserved sequence of DNA hsp65 was amplified by PCR and sequenced by universal primers of Mycobacterium, and inhA,rpoB,katG,embB,rpsL,rrs and pncA of HIV/AIDS patients were amplified and sequenced by nested PCR. To detect the mutation rate of drug-resistant genes and to analyze the proportion of "Beijing type" in tuberculous strains by RD105 (regions of difference105 deletion identification technique, and then to analyze the virulence of Mycobacterium tuberculosis infection. Results the positive and negative results of Mycobacterium DNA specimens isolated from 326 patients with HIV/AIDS were 244 and 82 respectively. The positive results included 167 strains of Mycobacterium tuberculosis (68.4%) and 77 strains of non-tuberculous mycobacterium (31.6%). Non-tuberculosis Mycobacterium included 39 strains of Mycobacterium Gordon, 18 strains of bird-intracellular mycobacterium complex, 12 strains of Mycobacterium Kansas, 3 strains of mycobacterium abscess, 2 strains of mycobacterium incidentally, 2 strains of Mycobacterium Marseilles and 1 strain of Xenopus. With the exception of no mutation in inhA, the mutation rates of the other six genes, rpoBnkatGlub, rpsLrrs and pncA, were 11.9and 7.7and 3.87.7and 3.86.1.82 strains of Mycobacterium tuberculosis co-infected with HIV/AIDS, respectively, of which 63 strains were "Beijing type", accounting for 76.8b; 19 strains were non-Beijing type, accounting for 23.1T. Conclusion Mycobacterium infection is one of the common complications in patients with HIV/AIDS. Mycobacterium complex and Mycobacterium Kansas are the four most common strains of Mycobacterium infection complications in HIV/AIDS patients. The rpoB,katG,embB,rpsL,rrs and pncA genes of HIV / AIDS patients have different mutation rates. Beijing type is one of the most virulent strains of Mycobacterium tuberculosis, and the most of the strains isolated from AIDS patients are Beijing type.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R512.91
本文編號：2200042
[Abstract]:Objective to obtain the species and composition of Mycobacterium coinfection in patients with HIV/AIDS. The mutation rate of drug resistance gene of Mycobacterium tuberculosis co-infected with HIV/AIDS was detected and the virulence of Mycobacterium tuberculosis was analyzed. Methods the conserved sequence of DNA hsp65 was amplified by PCR and sequenced by universal primers of Mycobacterium, and inhA,rpoB,katG,embB,rpsL,rrs and pncA of HIV/AIDS patients were amplified and sequenced by nested PCR. To detect the mutation rate of drug-resistant genes and to analyze the proportion of "Beijing type" in tuberculous strains by RD105 (regions of difference105 deletion identification technique, and then to analyze the virulence of Mycobacterium tuberculosis infection. Results the positive and negative results of Mycobacterium DNA specimens isolated from 326 patients with HIV/AIDS were 244 and 82 respectively. The positive results included 167 strains of Mycobacterium tuberculosis (68.4%) and 77 strains of non-tuberculous mycobacterium (31.6%). Non-tuberculosis Mycobacterium included 39 strains of Mycobacterium Gordon, 18 strains of bird-intracellular mycobacterium complex, 12 strains of Mycobacterium Kansas, 3 strains of mycobacterium abscess, 2 strains of mycobacterium incidentally, 2 strains of Mycobacterium Marseilles and 1 strain of Xenopus. With the exception of no mutation in inhA, the mutation rates of the other six genes, rpoBnkatGlub, rpsLrrs and pncA, were 11.9and 7.7and 3.87.7and 3.86.1.82 strains of Mycobacterium tuberculosis co-infected with HIV/AIDS, respectively, of which 63 strains were "Beijing type", accounting for 76.8b; 19 strains were non-Beijing type, accounting for 23.1T. Conclusion Mycobacterium infection is one of the common complications in patients with HIV/AIDS. Mycobacterium complex and Mycobacterium Kansas are the four most common strains of Mycobacterium infection complications in HIV/AIDS patients. The rpoB,katG,embB,rpsL,rrs and pncA genes of HIV / AIDS patients have different mutation rates. Beijing type is one of the most virulent strains of Mycobacterium tuberculosis, and the most of the strains isolated from AIDS patients are Beijing type.
【學(xué)位授予單位】：北京協(xié)和醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R512.91
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