本文選題：新城疫病毒 + 傳染性支氣管炎病毒�。� 參考：《中國病原生物學(xué)雜志》2017年08期

【摘要】：目的建立可同時檢測新城疫病毒(Newcastle disease virus,NDV)和傳染性支氣管炎病毒(Infectious bronchitis virus,IBV)的多重DNA微列陣鑒定方法。方法針對NDV和IBV設(shè)計PCR引物和微陣列探針,制備檢測用微陣列。將樣品與微列陣進(jìn)行雜交,分析微陣列的特異性和靈敏度。結(jié)果建立的微陣列檢測NDV和IBV為陽性,且能區(qū)分NDV和IBV,而流感病毒傳染性法氏囊病毒等陰性對照無雜交信號。通過GenePix 4.1軟件分析雜交信號的平均SNR532,NDV和IBV的檢出限為1×10~3copies/μl。結(jié)論建立的多重DNA微列陣鑒定方法具有高特異性和高敏感性,可用于NDV和IBV感染檢測。
[Abstract]:Objective to establish a multiplex bronchitis microarray method for simultaneous detection of Newcastle disease virus (NDV) and infectious bronchitis virus (IBV). Methods PCR primers and microarray probes were designed for NDV and IBV to prepare microarrays for detection. The samples were hybridized with microarray to analyze the specificity and sensitivity of the microarray. Results the established microarray was positive for NDV and IBV, and could distinguish NDV from IBV, while the negative control group of Infectious bursal virus (Infectious bursal virus) of influenza virus had no hybridization signal. Using GenePix 4.1 software, the detection limit of average SNR532 NDV and IBV was 1 脳 10~3copies/ 渭 L. Conclusion the multiplex DNA microarray method has high specificity and sensitivity and can be used to detect NDV and IBV infection.
【作者單位】： 長春中藥大學(xué)附屬醫(yī)院檢驗科;吉林大學(xué)臨床醫(yī)學(xué)院;
【基金】：吳階平醫(yī)學(xué)基金會臨床科研專項資助基金課題(No.320.6750.16068)
【分類號】：R440;R511
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本文編號：2058471