本文選題：肺炎克雷伯菌 + 敗血癥　； 參考：《中國(guó)病原生物學(xué)雜志》2015年01期

【摘要】：目的自地下生活污水中分離肺炎克雷伯菌的噬菌體,明確其生物學(xué)特性,觀察其對(duì)小鼠多重耐藥肺炎克雷伯菌所致敗血癥的療效。方法電鏡觀察LH-01形態(tài),調(diào)查其噬菌譜、生長(zhǎng)曲線等生物學(xué)特性。建立小鼠敗血癥感染模型,觀察LH-01的療效。結(jié)果 LH-01在其宿主菌的菌苔上形成毒性噬菌體所具有的完全透明的噬菌斑,電鏡觀察LH-01具典型的有尾噬菌體目短尾病毒科病毒的形態(tài)特征。一步生長(zhǎng)曲線顯示LH-01的潛伏期約為10min,裂解量約為100PFU/細(xì)胞。穩(wěn)定性試驗(yàn)顯示LH-01在pH 5~10及50℃和60℃環(huán)境均具良好穩(wěn)定性。LH-01治療克雷伯菌感染敗血癥小鼠的存活率為90%,對(duì)照組存活率為0,差異有統(tǒng)計(jì)學(xué)意義(P0.001),且無(wú)毒副作用。結(jié)論 LH-01具有高效的裂菌活性和高穩(wěn)定性,治療克雷伯菌感染小鼠敗血癥有效且安全,有望成為生物抗菌劑,其有效成分有待進(jìn)一步研究。
[Abstract]:Objective to isolate Klebsiella pneumoniae phage from subsurface sewage, to clarify its biological characteristics and observe its effect on septicaemia induced by Klebsiella pneumoniae in mice. Methods the morphology of LH-01 was observed by electron microscopy, and the biologic characteristics of the phage spectrum and growth curve were investigated. The infection model of septicemia in mice was established and the effect of LH-01 was observed. Results LH-01 formed completely transparent phage plaque on the bacteriophage of its host bacteria. The morphological characteristics of LH-01 with typical tailed phage virus were observed by electron microscopy. One step growth curve showed that the incubation period of LH-01 was about 10min and the amount of lysis was about 100PFU/ cells. The stability test showed that LH-01 was in pH 5~1. The survival rate of Klebsiella pneumoniae infected mice was 90% at 0, 50 and 60, and the survival rate of the control group was 0. The difference was statistically significant (P0.001), and there was no toxic side effect. Conclusion LH-01 has high efficiency and high stability. It is effective and safe to treat the septicaemia of Klebsiella infection in mice. It is hopeful and safe to treat the septicemia in mice infected with Klebsiella. As an antibacterial agent, its effective ingredients need further study.
【作者單位】： 河南醫(yī)學(xué)高等�？茖W(xué)校病原生物學(xué)與免疫學(xué)教研室;鄭州大學(xué)基礎(chǔ)醫(yī)學(xué)院病原生物學(xué)教研室;
【基金】：河南省科技廳重點(diǎn)科技攻關(guān)項(xiàng)目(No.142102310440) 河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃項(xiàng)目(No.201303199)
【分類號(hào)】：R515.3
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本文編號(hào)：1920920