本文選題：隱球菌 + 基因分型　； 參考：《四川大學學報(醫(yī)學版)》2015年01期

【摘要】：目的了解四川地區(qū)隱球菌臨床分離株基因分型及體外臨床常用抗真菌藥物敏感性情況。方法采用針對URA5基因PCR產物的限制性片段長度多態(tài)性(RFLP)方法對收集自我校華西醫(yī)院的92株隱球菌臨床分離株進行基因分型;采用E-test法檢測臨床常用抗真菌藥物兩性霉素B(AMB)、氟胞嘧啶(FC)、氟康唑(FCA)、伊曲康唑(ITR)和伏立康唑(VRC)對隱球菌分離株的最低抑菌濃度(MIC)范圍,并計算其MIC50、MIC90。結果92株隱球菌中,91株為新生隱球菌VNⅠ型,1株為格特隱球菌VGⅡ型。5種抗真菌藥物對92株隱球菌臨床株的MIC值范圍、MIC50、MIC90值分別如下:兩性霉素B為0.002~2μg/mL、0.19μg/mL和0.75μg/mL;氟胞嘧啶為0.5~32μg/mL、4μg/mL和8μg/mL;氟康唑為0.5~32μg/mL、3μg/mL和8μg/mL;伊曲康唑為0.064~2μg/mL、0.5μg/mL和1.5μg/mL;伏立康唑為0.004~0.19μg/mL、0.047μg/mL和0.094μg/mL。其中3株(3.3%)對兩性霉素B耐藥,4株(4.3%)對氟胞嘧啶耐藥,25株(27.2%)對伊曲康唑耐藥,未發(fā)現(xiàn)對氟康唑耐藥的菌株,所有菌株對伏立康唑敏感。格特隱球菌(1株)對氟胞嘧啶耐藥,對氟康唑劑量依賴敏感。不同時間段隱球菌對5種抗真菌藥物的MIC值比較,差異均具有統(tǒng)計學意義。隨時間推移,兩性霉素B及氟胞嘧啶的MIC值有所升高,唑類藥物MIC值變化無規(guī)律。結論四川地區(qū)隱球菌以新生隱球菌VNⅠ型為主,存在格特隱球菌VGⅡ型。除伊曲康唑外,隱球菌對其他抗真菌藥物敏感性高,僅少數(shù)菌株對兩性霉素B及氟胞嘧啶耐藥。
[Abstract]:Objective to investigate the genotyping of Cryptococcus clinical isolates and the susceptibility of common antifungal drugs in vitro. Methods 92 clinical isolates of Cryptococcus lucidum collected from Huaxi Hospital were genotyped by restriction fragment length polymorphism (RFLP) for PCR products of URA5 gene. The minimum inhibitory concentration (MIC) of amphotericin, flucytosine, fluconazole, itraconazole, itraconazole and vericonazole against Cryptococcus isolates was detected by E-test method, and its MIC50 MIC90 was calculated. Results among 92 Cryptococcus neoformans, 91 strains were Cryptococcus neoformans VN 鈪，

本文編號：1806909