本文選題：巴爾通體　切入點：鼠類　出處：《中國人獸共患病學(xué)報》2017年07期 　論文類型：期刊論文

【摘要】：目的了解福建省鼠類中巴爾通體(Bartonellaspp.)的感染狀況和基因特征。方法 2014-2016年采用籠日法在福建省閩東、閩西、閩南、閩北和閩中捕鼠,現(xiàn)場鑒定并記錄捕獲鼠類的懫集時間、地點、鼠種、性別、鼠齡等資料。采集鼠心臟血,PCR擴(kuò)增巴爾通體的gltA和16S~23SrRNA基因,陽性PCR產(chǎn)物送測序并進(jìn)行序列比對分析,構(gòu)建系統(tǒng)進(jìn)化樹。感染率間的比較采用χ~2檢驗或Fisher精確檢驗法。結(jié)果調(diào)查共布放鼠籠5 917籠次,捕鼠381只,鼠密度為6.44%。巴爾通體感染率為12.34%。家鼠的巴爾通體感染率為10.61%,褐家鼠(Rattus norvebicus)和黃胸鼠(Rattus flavipectus)感染率分別為11.30%和10.00%;野鼠的感染率為13.86%,黃毛鼠(Rattus losea)和針毛鼠(Rattus fulvescens)感染率分別為22.86%和18.00%,野鼠的巴爾通體感染率高于家鼠的感染率,但無統(tǒng)計學(xué)意義。從地區(qū)分布看,閩西、閩北一帶的感染率較高,分別為20.00%和25.33%。閩南一帶感染率最低,為0。各地區(qū)感染率存在統(tǒng)計學(xué)意義(P0.05)。不同性別、鼠齡的巴爾通體感染率差異無統(tǒng)計學(xué)意義,而不同的生境下的感染率存在統(tǒng)計學(xué)差異(P0.05)。陽性樣本測序分析顯示,福建省鼠類感染的巴爾通體序列與B.tribocorum、B.elizabethae和B.grahamii序列最接近。結(jié)論福建省鼠類存在巴爾通體感染,存在對人群致病的風(fēng)險。
[Abstract]:Objective to understand the infection status and genetic characteristics of Bartonella Bartonella pp. in Fujian Province. Methods the caged day method was used to catch mice from 2014 to 2016 in Mindong, Western Fujian, South Fujian, North Fujian and Central Fujian, and the time and location of catching rodents were identified and recorded in the field. The gltA and 16SN 23s rRNA genes of Baltonella were amplified by PCR from rat heart blood samples. The positive PCR products were sequenced and sequenced. A phylogenetic tree was constructed. The infection rate was compared by 蠂 ~ 2 test or Fisher accurate test. The density of rats was 6.44. The infection rate of Bartonella was 12.34.The infection rate of Bartonella was 10.61.The infection rates of Rattus norvebicusand Rattus flavipectus were 11.30% and 10.000.The infection rates of wild mice were 13.86b, Rattus losea) and Rattus fulvescens, respectively. The infection rate of Bartonella in wild mice was higher than that in house mice, But there was no statistical significance. According to the regional distribution, the infection rate in the west of Fujian and in the northern part of Fujian was higher, which was 20.00% and 25.33, respectively. The infection rate in the south of Fujian was the lowest, which was 0.The infection rate in each region was statistically significant (P 0.05). There was no significant difference in the infection rate of Baltonella in the age of mice, but there was a statistical difference in the infection rate in different habitats (P 0.05). The sequence of Bartonella was the closest to that of B. tribocorumum B.elizabethae and B.grahamii in Fujian Province.
【作者單位】： 福建省疾病預(yù)防控制中心福建省人獸共患病研究重點實驗室;福建醫(yī)科大學(xué);
【基金】：福建省醫(yī)學(xué)創(chuàng)新課題(No.2012-CXB-12) 福建省自然科學(xué)基金項目(No.2017J01139)聯(lián)合資助~~
【分類號】：R516

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 宋秀平;栗冬梅;賈麗軍;魯亮;王君;劉云彥;姜亞運(yùn);劉起勇;;內(nèi)蒙古小型獸類巴爾通體感染情況調(diào)查[J];中國媒介生物學(xué)及控制雜志;2015年03期

2 凌鋒;丁豐;龔震宇;陳恩富;侯娟;繆梓萍;施旭光;秦淑文;栗冬梅;劉起勇;;浙江省嚙齒動物巴爾通體感染狀況調(diào)查[J];中國媒介生物學(xué)及控制雜志;2014年01期

3 張振興;李玉峰;;巴爾通體病研究進(jìn)展[J];畜牧與獸醫(yī);2012年11期

4 栗冬梅;張建中;劉起勇;;中國巴爾通體與相關(guān)疾病的研究進(jìn)展[J];中國人獸共患病學(xué)報;2008年08期

5 葉曦;姚美琳;李國偉;;福建省鼠形動物巴爾通體感染調(diào)查[J];中國人獸共患病學(xué)報;2006年08期

6 栗冬梅,俞東征,劉起勇,海榮,郭炳衡;用分子生物學(xué)技術(shù)診斷巴爾通體感染[J];中華流行病學(xué)雜志;2004年07期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳寶寶;安翠紅;雷毅;范鎖平;栗冬梅;孫養(yǎng)信;;陜西省定邊縣鼠疫疫區(qū)嚙齒動物巴爾通體感染情況調(diào)查[J];中國衛(wèi)生檢驗雜志;2017年15期

2 肖方震;林代華;周淑Y，

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