本文選題：艾滋病　切入點(diǎn)：抗病毒治療　出處：《中國艾滋病性病》2015年10期 　論文類型：期刊論文

【摘要】：目的觀察北京地區(qū)未經(jīng)抗病毒治療的艾滋病病毒(HIV)感染者HIV-1毒株耐藥基因的變異情況,用于指導(dǎo)臨床用藥。方法以2014年4-7月北京地區(qū)601例未經(jīng)抗反轉(zhuǎn)錄病毒治療的HIV感染者/艾滋病(AIDS)病人(簡稱HIV/AIDS病人)為對象。采集病人的靜脈血,提取血漿病毒核糖核酸(RNA),用反轉(zhuǎn)錄/巢式聚合酶鏈?zhǔn)椒磻?yīng)擴(kuò)增病毒pol基因,并進(jìn)行序列測定和亞型分析。在Stanford University HIV耐藥數(shù)據(jù)庫查詢,分析是否存在基因型耐藥以及耐藥種類。結(jié)果 601例未經(jīng)抗反轉(zhuǎn)錄病毒治療的HIV/AIDS病人中,擴(kuò)增成功的458例,其中男性434例(94.8%),女性24例(5.2%);平均年齡33歲(17~77歲)。主要感染亞型依次為CRF01_AE(211例,46.1%)、CRF07_BC(126例,27.5%)、B亞型(106例,23.1%)和C亞型(15例,3.3%)。在擴(kuò)增成功的病人中,7.4%(34/458)的病人存在原發(fā)耐藥基因變異,其中7例病人對核苷類反轉(zhuǎn)錄酶抑制劑(NRTIs)耐藥,16例病人對非核苷類反轉(zhuǎn)錄酶抑制劑(NNRTIs)耐藥,8例對蛋白酶抑制劑(PIs)耐藥,3例病人同時對NRTIs和NNRTIs耐藥。結(jié)論北京地區(qū)未經(jīng)治療的HIV/AIDS病人中,有部分病人存在原發(fā)耐藥基因變異,極少數(shù)病人同時存在對兩種主要治療藥物耐藥,因此治療前需要根據(jù)耐藥檢測結(jié)果制定用藥方案,有助于提高治療有效率,避免治療失敗。
[Abstract]:Objective to observe the variation of drug resistance gene of HIV-1 strain in HIV infected persons without antiviral therapy in Beijing area. Methods A total of 601 patients with HIV / AIDS-AIDS (HIV/AIDS) who were not treated with antiretrovirals from April to July in Beijing from 2014 to July were studied. The venous blood of the patients was collected. The viral pol gene was amplified by reverse transcription / nested polymerase chain reaction (RT-PCR), and sequenced and subtype analyzed. Results among the 601 HIV/AIDS patients who were not treated with antiretroviral therapy, 458 were amplified successfully. Among them, 434 cases were male and 24 cases were female. The average age was 33 years old and 1777 years old. The main subtypes of infection were CRF01_AE(211 (46.1%), CRF07BC126 cases (10.6 cases) and C subtype (15 cases). There was genetic variation of primary drug resistance in the patients with successful amplification. Among them, 7 cases were resistant to nucleoside reverse transcriptase inhibitor (NRTIs) and 16 cases were resistant to NNRT Is.Conclusion in Beijing area, both NRTIs and NNRTIs are resistant to both NRTIs and NNRTIs in 8 cases. Of untreated HIV/AIDS patients, Some patients have genetic variation of primary drug resistance, and a few patients have two main drug resistance. Therefore, it is necessary to make drug use plan according to the results of drug resistance test before treatment, which is helpful to improve the effective rate of treatment and avoid the failure of treatment.
【作者單位】： 首都醫(yī)科大學(xué)附屬北京佑安醫(yī)院;北京市疾病預(yù)防控制中心;首都醫(yī)科大學(xué)附屬北京地壇醫(yī)院;
【基金】：國家自然科學(xué)基金(81273136) 北京市醫(yī)院管理局臨床醫(yī)學(xué)發(fā)展專項經(jīng)費(fèi)資助(ZY201401)~~
【分類號】：R512.91

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 Maria Au;Jessica Haberer;;Overview of HIV drug resistance and its implications for China[J];Chinese Medical Journal;2006年23期

2 韓曉旭,張e，

本文編號：1579766