本文關(guān)鍵詞： 日本血吸蟲(chóng) 腦型血吸蟲(chóng)病 磁共振成像 動(dòng)物模型 蟲(chóng)卵肉芽腫　出處：《中國(guó)血吸蟲(chóng)病防治雜志》2017年05期 　論文類型：期刊論文

【摘要】：目的建立家兔急性腦型血吸蟲(chóng)病模型,探討急性血吸蟲(chóng)腦病早期磁共振表現(xiàn)。方法家兔分3組,每組10只;實(shí)驗(yàn)組通過(guò)鉆顱法注射日本血吸蟲(chóng)蟲(chóng)卵懸浮液1 ml(0.9 mg);陰性對(duì)照組用相同方法經(jīng)顱注射生理鹽水1 ml;空白對(duì)照組不做任何處理。前兩組術(shù)后使用抗生素預(yù)防感染。觀察3組動(dòng)物的臨床表現(xiàn),并于術(shù)后30 d行頭顱磁共振檢查;隨后取腦組織制作病理切片,觀察腦組織病理改變。結(jié)果實(shí)驗(yàn)組家兔術(shù)后均出現(xiàn)食欲不振、精神異常,偏癱及體重下降等癥狀;陰性對(duì)照組家兔術(shù)后3 d內(nèi)均出現(xiàn)食欲下降,1周后消失,無(wú)異常精神癥狀;空白對(duì)照組無(wú)不良反應(yīng)。實(shí)驗(yàn)組10只家兔磁共振表現(xiàn)均發(fā)現(xiàn)異常,表現(xiàn)為T1WI增強(qiáng),出現(xiàn)片狀、結(jié)節(jié)狀強(qiáng)化,腦水腫,腦室擴(kuò)張,注射針道異常強(qiáng)化,SWI腦內(nèi)強(qiáng)化結(jié)節(jié)內(nèi)異常點(diǎn)低信號(hào)及手術(shù)側(cè)腦半球內(nèi)片狀低信號(hào)。陰性對(duì)照組2只磁共振表現(xiàn)為針道異常強(qiáng)化,1只腦室輕度擴(kuò)張�？瞻讓�(duì)照組磁共振表現(xiàn)無(wú)異常。實(shí)驗(yàn)組10只腦組織病理切片均發(fā)現(xiàn)異常,其中6只為血吸蟲(chóng)蟲(chóng)卵肉芽腫結(jié)節(jié)、非特異性肉芽腫結(jié)節(jié)以及血管周圍炎并存;陰性對(duì)照組未發(fā)現(xiàn)肉芽腫結(jié)節(jié),2只出現(xiàn)血管周圍炎;空白對(duì)照組腦組織切片正常。結(jié)論經(jīng)顱注射血吸蟲(chóng)蟲(chóng)卵可成功建立家兔急性血吸蟲(chóng)腦病模型;通過(guò)觀察磁共振圖像總結(jié)早期血吸蟲(chóng)腦病表現(xiàn),有助于提高早期診斷的正確率。
[Abstract]:Objective to establish an acute cerebral schistosomiasis model in rabbits and to investigate the early MRI manifestations of acute schistosomiasis. Methods Rabbits were divided into 3 groups with 10 rats in each group. In the experimental group, Schistosoma japonicum egg suspension was injected into the egg suspension of Schistosoma japonicum 1 ml(0.9 by drilling the skull. The negative control group was injected with 1 ml of normal saline by the same method. No treatment was given in the blank control group. The first two groups were treated with antibiotics to prevent infection after operation. The clinical manifestations of the three groups were observed and cranial magnetic resonance imaging was performed 30 days after operation. The pathological changes of brain tissue were observed. Results all the rabbits in the experimental group had symptoms of loss of appetite, mental abnormality, hemiplegia and weight loss after operation. In the negative control group, the appetite decreased within 3 days after operation and disappeared after 1 week, and no abnormal mental symptoms were found. There were no adverse reactions in the blank control group. All the 10 rabbits in the experimental group showed abnormal MRI findings, such as T 1WI enhancement, flake enhancement, nodular enhancement, cerebral edema, ventricular dilatation and abnormal enhancement of the injection needle. In SWI, the abnormal points in the nodules were hypointensity and the lamellar hypointensity in the operative hemispheres. In the negative control group, 2 cases showed abnormal enhancement of the needle tract. One brain ventricle was slightly dilated. There was no abnormal MRI in the blank control group. In the experimental group, 10 brain tissue sections showed abnormal findings, 6 of which were egg granuloma nodules of Schistosoma japonicum. Non-specific granulomatous nodules and perivascular inflammation coexist. No granulomatous nodules were found in the negative control group. Conclusion the rabbit model of acute schistosomiasis can be successfully established by transcranial injection of Schistosoma japonicum eggs. It is helpful to improve the accuracy of early diagnosis by observing magnetic resonance imaging to summarize the manifestations of early schistosomiasis encephalopathy.
【作者單位】： 江南大學(xué)附屬醫(yī)院;江南大學(xué)公共衛(wèi)生學(xué)院;武警浙江省總隊(duì)醫(yī)院放射科;江蘇省血吸蟲(chóng)病防治研究所;浙江中醫(yī)藥大學(xué)動(dòng)物實(shí)驗(yàn)研究中心;江南大學(xué)醫(yī)學(xué)院;
【基金】：江南大學(xué)公共衛(wèi)生研究中心立項(xiàng)項(xiàng)目(1286010242150640)
【分類號(hào)】：R-332;R532.21
【正文快照】： 江南大學(xué)公共衛(wèi)生研究中心立項(xiàng)項(xiàng)目(1286010242150640)1江南大學(xué)附屬醫(yī)院(無(wú)錫214000);2江南大學(xué)公共衛(wèi)生學(xué)院;3武警浙江省總隊(duì)醫(yī)院放射科;4江蘇省血吸蟲(chóng)病防治研究所;5浙江中醫(yī)藥大學(xué)動(dòng)物實(shí)驗(yàn)研究中心;6江南大學(xué)醫(yī)學(xué)院葛宇曦,女,研究生,主治醫(yī)師。研究方向:磁共振診斷*通信作，

本文編號(hào)：1473646