第一部分食管鱗癌順鉑耐藥細(xì)胞的建立和長鏈非編碼RNA NORAD的表達(dá)目的:通過建立食管鱗狀細(xì)胞癌(esophageal squamous cell cancer,ESCC)順鉑(cis-dichloro diammine platinum,CDDP)耐藥細(xì)胞株,檢測(cè)長鏈非編碼RNA(long non-coding RNA,lnc RNA)DNA損傷激活的RNA(Non-coding RNA activated by DNA damage,NORAD)在ESCC CDDP耐藥前后表達(dá)水平的變化,為深入研究食管癌CDDP耐藥機(jī)制奠定基礎(chǔ)。方法:通過體外間歇誘導(dǎo)和藥物濃度遞增的方法建立可在一定濃度CDDP培養(yǎng)液中維持生長的CDDP耐藥細(xì)胞株YES-2/CDDP-R。倒置顯微鏡觀察細(xì)胞形態(tài)學(xué)變化。MTT檢測(cè)YES-2和YES-2/CDDP-R細(xì)胞CDDP的半抑制濃度。實(shí)時(shí)熒光定量聚合酶鏈?zhǔn)椒磻?yīng)(quantitative real-time polymerase chain reaction,q RT-PCR)和蛋白質(zhì)印跡法(western blotting)檢測(cè)YES-2和YES-2/CDD...

【文章頁數(shù)】：67 頁

【學(xué)位級(jí)別】：碩士

【部分圖文】：

圖2CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞的影響

19圖2CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞的影響Fig.2EffectsofCDDPoncellofYES-2andYES-2/CDDP-Rcell.(*P<0.05,**P<0.01vs.ESCCcellsYES-2)3.YES-2/CDDP-R細(xì)胞中MRP1基....

圖3CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞中MRP1mRNA表達(dá)的影響

19圖2CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞的影響Fig.2EffectsofCDDPoncellofYES-2andYES-2/CDDP-Rcell.(*P<0.05,**P<0.01vs.ESCCcellsYES-2)3.YES-2/CDDP-R細(xì)胞中MRP1基....

圖4CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞中MRP1蛋白表達(dá)的影響

20圖4CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞中MRP1蛋白表達(dá)的影響Fig.4EffectofCDDPontheexpressionofMRP1proteininYES-2andYES-2/CDDP-Rcell.(*P<0.05,vs.ESCCcellsYES-2)....

圖5CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞遷移能力的影響

20圖4CDDP對(duì)YES-2和YES-2/CDDP-R細(xì)胞中MRP1蛋白表達(dá)的影響Fig.4EffectofCDDPontheexpressionofMRP1proteininYES-2andYES-2/CDDP-Rcell.(*P<0.05,vs.ESCCcellsYES-2)....

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