目的研究spv B/spv C基因?qū)ι抽T菌毒力及對宿主免疫系統(tǒng)的影響。方法野生型沙門菌(STM.211)、敲除spv B基因的沙門菌(STM.211-Δspv B)、敲除spv C基因的沙門菌(STM.211-Δspv C)、敲除spv B及spv C基因的沙門菌(STM.211-Δspv B.spv C)和PBS組分別經(jīng)腹腔注射0.2 m L105CFU的STM.211、STM.211-Δspv B、STM.211-Δspv C、STM.211-Δspv B.spv C感染BALB/c小鼠,觀察小鼠精神狀態(tài)、運動情況、腹瀉、體質(zhì)量、毛發(fā)改變等感染中毒癥狀,用ELISA法測定小鼠血清IL-10、IL-12、IFN-γ水平;并分離小鼠肝、脾,觀察靶器官大體觀及顯微鏡下的病理改變。結(jié)果(1)STM.211、STM.211-Δspv B和STM.211-Δspv C組的中毒癥狀明顯較PBS組嚴重,STM.211-Δspv B.spv C組與PBS組間無明顯差異;(2)STM.211組、STM.211-Δspv B組、STM.211-Δspv C組的IFN-γ和IL-12分泌量均顯著低于STM...

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圖1小鼠肝臟大體觀改變Fig.1Generalviewchangesoftheliver(3d).A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ΔspvB.spvC.Theliverofmicewhichinfectedwithsalmonellaareincreasedandcongestion,anddottednecroticABCDE

-ΔspvB、STM.211-spvCSTM.211-ΔspvB.spvC>PBS。2.2小鼠肝臟和脾臟的病理變化感染沙門菌后1d，各組間肝脾飽滿有光澤，大小與正常對照組無明顯差異。感染后3d起，分離小鼠肝脾，肉眼觀察發(fā)現(xiàn)感染沙門菌的小鼠的肝脾明顯增大并充血，肝臟表面出現(xiàn)點狀壞死....

圖2小鼠脾臟大體觀改變Fig.2Generalviewchangesofthespleen(3d).A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ABCDE

圖3小鼠肝臟光鏡下病理改變Fig.3Lightmicroscopyviewchangesoftheliver,3d（HE,Originalmagnification:×40）.A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ΔspvB.spvC.Thehepatocyteishydropicdegeneration,anditscollectabbacyinflammatorycellsinfiltrationandfocalpointofflakenecrosis.Thedegreeofthepathologicalchanges:STM.211＞DE

圖4小鼠脾臟光鏡下病理改變，3dFig.4Lightmicroscopyviewchangesofthespleen,3d（HE,Originalmagnification:×20）.A:PBS;B:STM.211;C:STM.211-ΔspvB;D:STM.211-ΔspvC;E:STM.211-ΔspvB.spvC.Thespleenhavemultinucleatedgiantcellhyperplasia，andthereisnoclearboundarybetweencortexandmedulla，splenicsinusexpansionhyperemia.Thedegreeofthepathological

，與IL-12共同誘導TH0細胞向TH1細胞分化，增強細胞免疫。缺乏IFN-γ有利于細菌繁殖，使宿主圖3小鼠肝臟光鏡下病理改變Fig.3Lightmicroscopyviewchangesoftheliver,3d（HE,Originalmagnification:×40）.A:....

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