1.1.1.研究背景 雄激素(androgen)主要指睪酮(testosterone, T)和其在體內的活性代謝產物雙氫睪酮(dihydrotestosterone, DHT)。睪酮由睪丸Leydig細胞產生并分泌,其合成和分泌過程受下丘腦-垂體-性腺軸及其它神經體液因素的嚴密調控。新生成的睪酮進入血液循環(huán)后,大部分與性激素結合球蛋白(sex hormone binding globulin, SHBG)結合形成復合物,不能進入組織和細胞;只有約1-2%呈游離狀態(tài)(血漿濃度約為19.9±4.7nM),能為組織所利用,發(fā)揮內分泌調節(jié)作用。組織和細胞中的5α-還原酶可將睪酮還原成雙氫睪酮,后者與雄激素受體的親和力要高于睪酮本身。雄激素在生殖系統(tǒng)的作用是:促進胚

【文章頁數(shù)】：85 頁

【學位級別】：博士

【文章目錄】：
Part 1 Abstracts
    1.1 Abstracts in Chinese
        1.1.1. Research background
        1.1.2. Resarch purpose
        1.1.3. Research protocals
        1.1.4. Results
        1.1.5. Conclusion
        1.1.6. Key wprds
    1.2. Abstracts in English
Part 2 Introduction
Part 3 Methods and Materials
    3.1. Mice
    3.2. Cell cultures
    3.3. Purity assay of BMMs
    3.4. Treatment of BMMs
    3.5. Reverse-transcriptase polymerase chain reaction (RT-PCR)
    3.6. Western blotting assay
    3.7. Determination of [Ca²⁺]
    3.8. Visualization of intracellular androgen receptor
    3.9. Labeling with testosterone-BSA-FITC
    3.10. Confocal laser scanning microscopy assay
    3.11. Statistical analysis
Part 4 Results
    4.1. Assessment of the Purity of BMMs
    4.2. Expression analysis of Notch in testosterone-treated BMMs
    4.3. Regulations of testosterone on LPS-induced Notch expression
    4.4. Testosterone-induced Ca²⁺ mobilization
    4.5. Absence of androgen receptor
    4.6. Testosterone binding sites on BMMs
Part 5 Discussion
    5.1. Effects of testosterone on BMMs
    5.2. Mechanism of testosterone action in macrophages
Part 6 Conclusion
Part 7 References
Part 8 Achievements (in Chinese)
Part 9 Review
    9.1. Secretion and production of androgen
    9.2. Biological effects of androgen
    9.3. Testosterone and immune response
    9.4. Testosterone and macrophage
    9.5. Mechanism of testosterone action
    9.6. Receptors for Rapid Androgen Signaling
    9.7. Members of Notch signaling pathway
    9.8 Perspectives
Part 10 Statement
Part 11 Acknowledgements



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