使用溴氰菊酯和生物丙烯菊酯對采自北京的淡色庫蚊蚊株進(jìn)行抗藥性篩選,篩選時,4齡幼蟲被置于LC₅₀濃度值的水體中飼養(yǎng)。經(jīng)過7代的不間斷篩選,蚊蟲對溴氰菊酯和生物丙烯菊酯的抗性倍數(shù)分別上升了12.9倍和4.8倍,對應(yīng)敏感株,抗性倍數(shù)分別為217.8倍和41.6倍。PCR檢測結(jié)果顯示,kdr抗性基因的頻率在逐代上升。F₇代時,溴氰菊酯篩選株和生物丙烯菊酯篩選株的kdr抗性基因頻率已分別上升至98.96%和97.87%。結(jié)果表明,Phe1014的抗性純合子頻率和抗性雜合子頻率均與LC₅₀值之間具有顯著相關(guān)性。本文證明了高濃度的擬除蟲菊酯殺蟲劑篩選會使Phe1014突變頻率快速的上升。 

【文章來源】：寄生蟲與醫(yī)學(xué)昆蟲學(xué)報(bào). 2019,26(03)

【文章頁數(shù)】：7 頁

【文章目錄】：
1 Materials and methods
    1.1 Mosquito strains
    1.2 Bioassay
    1.3 Pyrethroid selection in mosquito strain
    1.4 Genomic DNA isolation and PCRamplification of the specific allele（PASA）assay for kdr genotype
    1.5 Statistical analysis
2 Results
    2.1 Resistance development in deltamethrin-and esbiothrin-selected populations
    2.2 Genotypes of Cx.pipiens pallens samples
    2.3 Relationship of Phe1014 frequency and LC50
3 Discussion



本文編號：3049067