本文選題：尿道下裂 + 小鼠�。� 參考：《山西醫(yī)科大學》2008年碩士論文

【摘要】： 第一部分大豆異黃酮致小鼠尿道下裂動物模型的建立 目的:應用大豆異黃酮孕晚期染毒誘導子代雄鼠尿道下裂的建立,為雌激素致尿道下裂的分子作用機制提供進一步研究的基礎(chǔ)。 方法:45只孕鼠隨機分成5組,在孕12-17d連續(xù)6d各組每晨9點分別灌胃生理鹽水0.2ml/d(A)、己烯雌酚0.5 mg/(kg.d)(B)、大豆異黃酮100 mg/(kg.d)(C)、300 mg/(kg.d)(D)、900 mg/(kg.d)(E),出生時觀察小鼠死亡率,出生后4周觀察小鼠有無尿道下裂。 結(jié)果:A,B,C,D,E各組平均死亡率分別為2.0%,18%,3.8%,5.4%,10%。A,B,C,D,E組子代尿道下裂發(fā)生率分別為0%(0/80),28%(11/39),0%(0/76),0%(0/67),19%(10/52)。E組子代尿道下裂發(fā)生率與A,C,D組比較有統(tǒng)計學差異(P＜0.05)。E組子代尿道下裂發(fā)生率與B組比較無統(tǒng)計學差異。 結(jié)論:應用昆明種小鼠從孕12d開始給予大豆異黃酮900 mg/(kg.d)灌胃,連續(xù)給藥6d,成功建立小鼠先天性尿道下裂模型。 第二部分先天性尿道下裂小鼠血清睪酮和雌二醇含量的測定 目的:測定小鼠血清睪酮和雌二醇的含量,初步探討大豆異黃酮致雄性仔鼠尿道下裂的發(fā)生機制。 方法:隨機選取空白對照組雄性仔鼠10只(F),從大豆異黃酮低(G)、中劑量組(H)各選取非尿道下裂雄性仔鼠10只,從大豆異黃酮高劑量組選取先天性尿道下裂雄性仔鼠10只(I),分別采血用放射免疫分析法測定睪酮(T)和雌二醇(E2),各組之間進行比較。 結(jié)果:F,G,H,I,4組體內(nèi)睪酮含量比較有顯著統(tǒng)計學差異性(P＜0.05),而雌二醇含量無明顯統(tǒng)計學差異性(P＞0.05)。 結(jié)論:先天性尿道下裂仔鼠與正常對照組比較,體內(nèi)睪酮含量比較有顯著統(tǒng)計學差異性,而雌二醇含量無明顯統(tǒng)計學差異性。 第三部分先天性尿道下裂小鼠陰莖皮膚及睪丸組織中AR的測定 目的:研究大豆異黃酮對雄性仔鼠尿道下裂的發(fā)生并進一步檢測仔鼠陰莖皮膚和睪丸組織中雄激素受體(AR)的表達,初步探討其發(fā)病機制。 方法:隨機選取正常對照組雄性仔鼠(J),造模組非尿道下裂仔鼠(K),先天性尿道下裂仔鼠各10只(L),分別取其陰莖皮膚和睪丸組織用免疫組織化學法測定AR的含量。 結(jié)果:L組陰莖皮膚組織中AR含量明顯低于J組和K組(P＜0.05),J組和L組間陰莖皮膚組織中AR含量無明顯差異(P＞0.05);而睪丸組織中AR含量三組總體無明顯差異性(P＞0.05)。 結(jié)論:大豆異黃酮誘導小鼠尿道下裂的發(fā)生,可能是由于大豆異黃酮導致小鼠陰莖皮膚組織AR的表達下降,從而導致雄激素和其受體的結(jié)合下降所致。
[Abstract]:Establishment of Mouse Model of Hypohypospadia Induced by First Part of Soybean isoflavone


Objective : To establish the mechanism of molecular mechanism of estrogen - induced hypospadia , which is the basis for further study .


Methods : Forty - five pregnant mice were randomly divided into five groups . At 9 o ' clock every morning , 0.2 ml / d ( A ) , 0.5 mg / kg ( kg 路 d ) ( B ) , 100 mg / ( kg 路 d ) ( C ) , 300 mg / ( kg 路 d ) ( D ) , 900 mg / ( kg 路 d ) ( E ) were observed in each group .


Results : The average mortality rates of group A , B , C , D and E were 2.0 % , 18 % , 3.8 % , 5.4 % , 10 % , A , B , C , D and E groups were 0 % ( 0 / 80 ) , 28 % ( 11 / 39 ) , 0 % ( 0 / 76 ) , 0 % ( 0 / 67 ) , 19 % ( 10 / 52 ) .


Conclusion : Using Kunming mice to administer soybean isoflavone 900 mg / ( kg 路 d ) at the beginning of gestation , the model of congenital hypospadia of mice was established successfully for 6 days .


Determination of serum testosterone and estradiol in the second part of children with congenital hypospadia


Objective : To determine the content of serum testosterone and estradiol in mice .


Methods : 10 male rats ( F ) were randomly selected from the group consisting of soybean isoflavone ( G ) and medium dose group ( H ) . 10 rats were selected from the low ( G ) and medium dose groups ( H ) , 10 ( I ) were selected from the high dose group of soybean isoflavone , the testosterone ( T ) and estradiol ( E2 ) were determined by radioimmunoassay , and the groups were compared .


Results : The levels of testosterone in F , G , H , I and 4 groups were significantly different ( P < 0 . 05 ) , but there was no statistically significant difference in estradiol content ( P > 0 . 05 ) .


Conclusion : Compared with the normal control group , the content of testosterone in the patients with congenital hypospadia is significantly different , while there is no statistically significant difference in the content of estradiol .


Determination of AR in the Penile Skin and Testicular Tissue of the Third Part of Congenital Hypohypospadia Mice


Objective : To study the effect of soybean isoflavone on hypospadia of male rats and to further detect androgen receptor ( AR ) expression in rat penis skin and testis tissue .


Methods : The contents of AR were determined by immunohistochemical method for the penile skin and testis tissues of the normal control group ( J ) , the model group ( K ) and 10 ( L ) of the congenital hypospadia , respectively .


Results : The AR content in penile skin of L group was significantly lower than that in group J and K ( P < 0 . 05 ) , there was no significant difference in AR content in penile skin tissue between group J and L ( P > 0 . 05 ) .


Conclusion : Soybean isoflavones induce the occurrence of hypospadia in mice , which may result from decreased expression of AR in the penile skin of mice due to soybean isoflavone , resulting in a decrease in the binding of androgen and its receptor .
【學位授予單位】：山西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2008
【分類號】：R-332;R726.9

【引證文獻】

相關(guān)期刊論文 前1條

1 袁耀美;張茨;白晨;;不同劑量氟他胺誘導小鼠建立尿道下裂及隱睪模型研究[J];新鄉(xiāng)醫(yī)學院學報;2012年04期

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本文編號：1987290