rno-miR-124-3p在大鼠急性持續(xù)性腦缺血過程中對MCT1表達的調(diào)控作用
發(fā)布時間：2021-08-10 16:53
第一部分 MCT1在急性持續(xù)性腦缺血大鼠腦組織中的表達變化目的:觀察MCT1（monocarboxylate transporter 1,MCT1）在急性持續(xù)性腦缺血大鼠腦組織中的表達變化。方法:1.健康成年雄性SD大鼠,大鼠隨機分為假手術(shù)組（n=10）和模型組（n=50）,模型組又分為5個亞組,分別于造模成功后1 h,3 h,6 h,12 h,24 h取材。2.運用線栓法制作持續(xù)性大腦中動脈栓塞（permanent middle cerebral artery occlusion,p MCAO）大鼠腦缺血模型,并進行行為學(xué)觀察。3.運用TTC染色法,觀察缺血后不同時段腦組織的缺血情況變化。4.運用免疫熒光技術(shù),激光共聚焦檢測MCT1在大鼠腦組織中的表達;運用熒光雙標法檢測MCT1與星形膠質(zhì)細胞標記物GFAP（Glial fibrillary acidic protein,GFAP）的共表達情況。5.運用Western blot技術(shù),檢測腦缺血后不同時段大鼠腦組織MCT1蛋白表達變化。結(jié)果:1.構(gòu)建p MCAO模型后大鼠的行為學(xué)變化如下:1 h內(nèi)大鼠尚未蘇醒,3 h后大鼠均已蘇醒,提起...
【文章來源】：重慶醫(yī)科大學(xué)重慶市
【文章頁數(shù)】：94 頁
【學(xué)位級別】：博士
【部分圖文】：
大鼠pMCAO模型制作和模型成功后大鼠的癥狀及取材所見
圖 1-2 尼氏染色檢測 pMCAO-24 h 后腦組織神經(jīng)元的變化。A1 和 A2 示大腦皮質(zhì)頂葉附近皮質(zhì)的尼氏染色結(jié)果。B1 和 B2 示大腦皮質(zhì)靠近側(cè)腦室的尼氏染色結(jié)果。C1 和 C2 示大腦皮質(zhì)顱底附近皮質(zhì)的尼氏染色結(jié)果。在梗塞側(cè)，上述三個區(qū)域都僅能看到一些破碎的染色較淺的細胞碎片，不能看到神經(jīng)元的形態(tài)；在對照側(cè)，則可清楚地看到染成紫藍色的神經(jīng)元及 Nisslbody。Fig. 1-2 Nissl staining detecting of the changes of neurons in the brain after 24 h of pMCAO.A1 and A2 show the nissl staining results of the cortex near the parietal cortex. B1 and B2showed the results of nissl staining of cerebral cortex near lateral ventricle.C1 and C2 show theresults of nissl staining in the cortex near the cerebral cortex base.In the above three areas onthe infarct side, only some fragmented and lightly stained cell fragments could be seen, but themorphology of neurons could not be seen.On the control side, the purple-blue stained neuronsand Nissl body were clearly seen.
圖 1-3 假手術(shù)及缺血后 1 h、3 h、6 h、12 h、24 h 時的 TTC 染色。缺血區(qū)顯示為白色。在假手術(shù)組大鼠的腦組織內(nèi)沒有蒼白色缺血灶出現(xiàn)；從缺血 1 h 開始，皮質(zhì)部分可觀察到缺血區(qū)，隨著缺血時延長至 24 h，缺血區(qū)域面積逐漸增大至梗塞側(cè)全部腦區(qū)。Fig. 1-3 TTC staining at 1 h, 3 h, 6 h, 12 h, and 24 h after ischemia and those of sham group. Theischemic areas are shown in white. No pale ischemic lesions were found in the brain tissue of thesham group. Ischemic regions can be observed in the cortex from after 1 h ischemia, and theischemic area gradually increased to the entire cerebral area on the infarct side upto after 24 hischemia.
本文編號：3334434
【文章來源】：重慶醫(yī)科大學(xué)重慶市
【文章頁數(shù)】：94 頁
【學(xué)位級別】：博士
【部分圖文】：
大鼠pMCAO模型制作和模型成功后大鼠的癥狀及取材所見
圖 1-2 尼氏染色檢測 pMCAO-24 h 后腦組織神經(jīng)元的變化。A1 和 A2 示大腦皮質(zhì)頂葉附近皮質(zhì)的尼氏染色結(jié)果。B1 和 B2 示大腦皮質(zhì)靠近側(cè)腦室的尼氏染色結(jié)果。C1 和 C2 示大腦皮質(zhì)顱底附近皮質(zhì)的尼氏染色結(jié)果。在梗塞側(cè)，上述三個區(qū)域都僅能看到一些破碎的染色較淺的細胞碎片，不能看到神經(jīng)元的形態(tài)；在對照側(cè)，則可清楚地看到染成紫藍色的神經(jīng)元及 Nisslbody。Fig. 1-2 Nissl staining detecting of the changes of neurons in the brain after 24 h of pMCAO.A1 and A2 show the nissl staining results of the cortex near the parietal cortex. B1 and B2showed the results of nissl staining of cerebral cortex near lateral ventricle.C1 and C2 show theresults of nissl staining in the cortex near the cerebral cortex base.In the above three areas onthe infarct side, only some fragmented and lightly stained cell fragments could be seen, but themorphology of neurons could not be seen.On the control side, the purple-blue stained neuronsand Nissl body were clearly seen.
圖 1-3 假手術(shù)及缺血后 1 h、3 h、6 h、12 h、24 h 時的 TTC 染色。缺血區(qū)顯示為白色。在假手術(shù)組大鼠的腦組織內(nèi)沒有蒼白色缺血灶出現(xiàn)；從缺血 1 h 開始，皮質(zhì)部分可觀察到缺血區(qū)，隨著缺血時延長至 24 h，缺血區(qū)域面積逐漸增大至梗塞側(cè)全部腦區(qū)。Fig. 1-3 TTC staining at 1 h, 3 h, 6 h, 12 h, and 24 h after ischemia and those of sham group. Theischemic areas are shown in white. No pale ischemic lesions were found in the brain tissue of thesham group. Ischemic regions can be observed in the cortex from after 1 h ischemia, and theischemic area gradually increased to the entire cerebral area on the infarct side upto after 24 hischemia.
本文編號：3334434
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