【摘要】：目的探討Notch1在小鼠腦出血(intracerebral hemorrhage,ICH)后對海馬神經(jīng)干細胞(neural stem cells,NSCs)分裂方式影響中的作用以及對小鼠ICH后遠期神經(jīng)功能恢復的影響。方法利用免疫熒光、Western blot、轉(zhuǎn)輪實驗和平衡木實驗等方法檢測小鼠腦出血后海馬NSCs的分裂方式情況、Notch1蛋白表達情況和神經(jīng)功能恢復情況。結(jié)果與Sham組比較,ICH導致小鼠海馬NSCs水平分裂的比例明顯降低(P0.01),同時發(fā)現(xiàn)小鼠海馬中Notch1的表達也明顯降低(P0.05)。促進ICH小鼠海馬中Notch1的表達能夠有效地增加小鼠海馬NSCs水平分裂的比例(P0.01),維持NSCs的數(shù)量,同時能夠有效地增加ICH后小鼠轉(zhuǎn)輪實驗掉落的潛伏期以及小鼠海馬中DCX陽性細胞數(shù)(P0.01),維持神經(jīng)發(fā)生的持續(xù)性,另外還能夠減少ICH后小鼠平衡木行走實驗腿的滑落次數(shù)(P0.01)。結(jié)論 Notch1信號可以調(diào)控腦出血后小鼠海馬NSCs的分裂方式以及促進遠期神經(jīng)功能恢復。
[Abstract]:Objective to investigate the effect of Notch1 on the division of hippocampal neural stem cells (neural stem cells,NSCs) after intracerebral hemorrhage (intracerebral hemorrhage,ICH) in mice and the long-term recovery of neural function after ICH in mice. Methods Immunofluorescence, Western blot, wheel test and balance beam test were used to detect the division mode, Notch1 protein expression and neural function recovery of hippocampal NSCs after intracerebral hemorrhage in mice. Results compared with Sham group, the proportion of NSCs level division induced by ICH in hippocampus of mice was significantly decreased (P 0.01), and the expression of Notch1 in hippocampus of mice was also significantly decreased (P 0.05). Promoting the expression of Notch1 in hippocampus of ICH mice could effectively increase the proportion of NSCs horizontal division in hippocampus of mice (P01), maintain the number of NSCs, and effectively increase the latency of wheel test drop and the number of DCX positive cells in hippocampus of mice after ICH (P01), maintain the persistence of neurogenesis, and reduce the number of slippage times of balance beam walking test legs after ICH (P01). Conclusion Notch1 signal can regulate the division mode of hippocampal NSCs and promote the recovery of long-term nerve function in mice after intracerebral hemorrhage.
【作者單位】： 寶雞高新人民醫(yī)院神經(jīng)內(nèi)科;第三軍醫(yī)大學新橋醫(yī)院神經(jīng)內(nèi)科;
【分類號】：R743.34

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