【摘要】：目的探討紅曲素(monascin)在腦出血后腦保護(hù)作用的機(jī)制及最佳劑量。方法 2016年3月—2016年10月于山西醫(yī)科大學(xué)神經(jīng)電生理實(shí)驗(yàn)室選取雄性SD大鼠120只,隨機(jī)分為假手術(shù)組、模型組、低劑量紅曲素組、中劑量紅曲素組、高劑量紅曲素組各24只。采用Ⅳ型膠原酶定位注射制備大鼠腦出血模型,于術(shù)后1、3、7、14 d行Garcia神經(jīng)功能缺損評分,行腦水含量測定、鐵染色及PPAR-γ、Nrf2、SRA測定。結(jié)果與假手術(shù)組相比,模型組、低劑量組、中劑量組及高劑量組神經(jīng)功能缺損評分在1、3、7、14 d顯著降低(F=181.705、494.229、226.576、24.726,P0.01),且在1、3 d評分最低,腦水含量在1、3 d顯著增高(F=21.781、70.036,P0.05),鐵沉積顆粒量在3、7、14 d顯著增多(F=21.426、118.122、107.773,P0.05),且于7~14 d達(dá)高峰,PPAR-γ免疫陽性細(xì)胞數(shù)于1、3、7 d顯著增多(F=3.394、28.622、14.12,P0.05),在3~7 d達(dá)高峰,Nrf2免疫陽性細(xì)胞數(shù)于1、3、7、14 d增多(F=33.076、23.17、47.779、12.182,P0.05),SRA免疫陽性細(xì)胞數(shù)于1、3、7、14 d顯著增多(F=22.283、511.092、51.811、31.060,P0.05)。與模型組相比,高劑量組Garcia評分在3~7 d時(shí)升高(P0.05),腦含水量及鐵染色顆粒量降低(P0.05),高劑量組PPAR-γ、Nff2及SPA免疫陽性細(xì)胞數(shù)于1、3及7 d明顯升高(P0.05),3~7 d達(dá)高峰,14 d降至模型組水平。結(jié)論高劑量紅曲素可增加腦出血后PPAR-γ、Nrf2及SPA陽性細(xì)胞數(shù),減輕腦出血后腦水腫及腦鐵含量,改善腦出血后神經(jīng)功能缺損。
[Abstract]:Objective to investigate the protective mechanism and optimal dose of Monascus (monascin) in the brain after intracerebral hemorrhage (ICH). Methods from March 2016 to October 2016, 120 male SD rats were randomly divided into sham operation group, model group, low dose Monascus group, middle dose Monascus group and high dose Monascus group. The rat model of intracerebral hemorrhage was established by localized injection of type IV collagenase. Garcia neurological deficit score, brain water content, iron staining, PPAR- 緯 and Nrf2,SRA were measured at 1, 3, 7 and 14 days after operation. Results compared with the false operation group, the neurological deficit scores of the model group, the low dose group, the middle dose group and the high dose group were significantly decreased at the 1st, 7th and 14th day (F 鈮，

本文編號(hào)：2499947