【摘要】：目的評(píng)估中腦黑質(zhì)損傷后內(nèi)源性神經(jīng)前體細(xì)胞(NPCs)的增殖情況及其對(duì)黑質(zhì)-紋狀體系統(tǒng)損傷后恢復(fù)的促進(jìn)作用。方法向成年小鼠的一側(cè)黑質(zhì)(SN)注射六羥多巴胺(6-OHDA),損傷后3~35 d運(yùn)用免疫熒光染色等方法,研究小鼠來自側(cè)腦室、第三腦室、中腦水管周圍及中腦部分NPCs的增殖,探索黑質(zhì)中新生細(xì)胞的增殖及其向成熟神經(jīng)元、多巴胺能神經(jīng)元分化的情況,最后通過曠場(chǎng)和轉(zhuǎn)棒實(shí)驗(yàn)檢測(cè)小鼠行為學(xué)的變化(每組n=4~6)。結(jié)果黑質(zhì)內(nèi)注射6-OHDA引起的多巴胺能神經(jīng)元損失可以明顯增加第三腦室和中腦水管周圍來自室管膜下區(qū)的NPCs的數(shù)目,以6-OHDA注射后的第7天最為明顯,且6-OHDA注射后第21天黑質(zhì)內(nèi)新生細(xì)胞和新生多巴胺能神經(jīng)元的數(shù)目增加達(dá)到高峰,這些變化可能導(dǎo)致了受損的黑質(zhì)-紋狀體系統(tǒng)及小鼠行為學(xué)表現(xiàn)有部分恢復(fù)。結(jié)論促進(jìn)內(nèi)源性NPCs的增殖和分化將成為治療帕金森病的理想手段。
[Abstract]:Objective to evaluate the proliferation of endogenous neural precursor cells (NPCs) after midbrain substantia nigra injury and its effect on the recovery of substantia nigra-striatum system. Methods the proliferation of NPCs from lateral ventricle, third ventricle, periaqueduct and mesencephalon of adult mice was studied by immunofluorescence staining at 3: 35 days after injury by injecting hexachloramine (6-OHDA) into one side of substantia nigra (SN) of adult mice. the proliferation of HCA from lateral ventricle, third ventricle, periaqueduct of midbrain and part of midbrain was studied by immunofluorescence staining. To explore the proliferation of neonatal cells in substantia nigra and its differentiation into mature neurons and dopaminergic neurons. Finally, the behavioral changes of mice were detected by open field and rod test (n 鈮，

本文編號(hào)：2491529