【摘要】：目的觀察下調(diào)LSINCT5對人腦膠質(zhì)瘤細胞系U251增殖、遷移能力和放療敏感性的影響。方法采用慢病毒轉染法構建穩(wěn)定干擾LSINCT5的細胞株(U251 sh LSINCT5細胞),得到干擾效率高的sh LSINCT5 1#組、sh LSINCT5 3#組和轉染GV248的對照組細胞進行后續(xù)實驗。采用噻唑藍比色法(MTS)觀察細胞培養(yǎng)0、24、48、72h時增殖能力變化,劃痕修復實驗觀察細胞遷移能力的變化,Western blotting法檢測細胞中細胞黏附分子(E-cadherin)、緊密連接蛋白-1(Zo-1)、細胞骨架蛋白(Vimentin)表達,MTS法觀察X射線儀下分別照射0、2、4、8 Gy后細胞增殖能力。結果 sh LSINCT5 1#組、sh LSINCT5 3#組培養(yǎng)48、72 h時細胞增殖能力均較對照組低(P均0.05)。sh LSINCT5 1#組、sh LSINCT5 3#組細胞的修復距離均較對照組短,P均0.05。與對照組相比,sh LSINCT51#組、sh LSINCT5 3#組E-cadherin、Zo-1表達高,Vimentin表達低(P均0.05)。sh LSINCT5 1#組、sh LSINCT5 3#組在2、4 Gy劑量照射時細胞增殖能力均較對照組低(P均0.05)。結論下調(diào)人膠質(zhì)瘤細胞系U251中LSINCT5的表達,能抑制細胞增殖、轉移,并增強細胞放療敏感性。
[Abstract]:Objective to observe the effect of down-regulation of LSINCT5 on proliferation, migration and radiotherapy sensitivity of human glioma cell line U251. Methods the cell lines stably interfering with LSINCT5 (U251 sh LSINCT5 cells) were constructed by lentivirus transfer method, and the cells in sh LSINCT5 1 # group, sh LSINCT5 3 # group and GV248 transgenic control group with high interference efficiency were obtained. (MTS) assay was used to observe the proliferation of cells cultured for 0, 24, 48 and 72 h, and the cell migration ability was observed by scratch repair assay. Cell adhesion molecule (E-cadherin) was detected by, Western blotting assay. The expression of tight junction protein-1 (Zo-1) and cytoskeleton protein (Vimentin) were observed by MTS assay. The proliferation ability of the cells was observed by MTS assay after irradiation with 0, 4 and 8 Gy, respectively. Results the proliferation ability of cells in sh LSINCT5 1 # group and sh LSINCT5 3 # group was lower than that in control group at 72 h (P < 0.05). Sh LSINCT5 1 #). The repair distance of cells in sh LSINCT5 3 # group was shorter than that in control group (P 0.05). Compared with the control group, the expression of Zoo1 and Vimentin in sh LSINCT51# group and sh LSINCT5 3 # group were higher and lower than those in control group (all P 0.05). Sh LSINCT51# group, P < 0.05). The proliferation ability of sh LSINCT5 3 # group was lower than that of the control group at the dose of 2 and 4 Gy (P < 0.05), and the cell proliferation ability of the control group was lower than that of the control group (P < 0.05). Conclusion the down-regulation of LSINCT5 expression in human glioma cell line U251 can inhibit the proliferation and metastasis of human glioma cell line U251 and enhance the radiosensitivity of human glioma cell line U251.
【作者單位】： 海南醫(yī)學院第二附屬醫(yī)院;海南醫(yī)學院;
【分類號】：R739.41

【相似文獻】

相關期刊論文 前10條

1 向莉;胡亞梅;張杰文;;美洲商陸抗病毒蛋白在畢赤酵母中的表達及其誘發(fā)人神經(jīng)膠質(zhì)瘤細胞U251凋亡的研究[J];中國中西醫(yī)結合雜志;2011年08期

2 閻華;胡新華;肖紅;鄒元杰;章文斌;劉宏毅;;不同放療敏感性膠質(zhì)瘤組織蛋白質(zhì)組學比較研究[J];臨床神經(jīng)外科雜志;2011年01期

3 涂艷陽;付建芳;徐如祥;王伯良;曹義戰(zhàn);仲月霞;付國強;田小溪;;尖吻蝮蛇小分子多肽對人神經(jīng)膠質(zhì)瘤細胞系U251的凋亡及bcl-2/bax基因表達和氧化應激的影響[J];現(xiàn)代生物醫(yī)學進展;2011年S2期

4 戴丹菁;謝叢華;張阿麗;邱慧兵;周遵艷;;TRAIL聯(lián)合放射線誘導人腦膠質(zhì)瘤細胞株U251凋亡的協(xié)同作用研究[J];武漢大學學報(醫(yī)學版);2006年06期

5 王文新;邱建武;;二硫代氨基甲酸吡咯烷聯(lián)合卡鉑對U251神經(jīng)膠質(zhì)瘤細胞增殖、凋亡的影響[J];重慶醫(yī)學;2013年07期

6 楊勇;彭浩;陳超波;虞浩;張志堅;龔愛華;王存祖;陳永昌;;神經(jīng)生長因子及其受體在人腦膠質(zhì)瘤細胞株U251中的表達及其生物學意義[J];中國臨床康復;2006年38期

7 王彬;楊輝;尹昌林;呂勝青;劉海鵬;;惡性膠質(zhì)瘤細胞株U251中腫瘤干細胞的分離、培養(yǎng)及鑒定[J];中國臨床神經(jīng)科學;2007年06期

8 湯浩;程彥磊;張本平;趙婕;方峻;;丹皮酚影響依托泊苷對人膠質(zhì)母細胞瘤細胞U251的細胞毒性[J];中國醫(yī)院藥學雜志;2012年09期

9 楚勝華;馬延斌;馮東福;邱建華;張紅;朱志安;;SLC22A18基因轉染人膠質(zhì)瘤U251細胞對放療敏感性的影響[J];現(xiàn)代腫瘤醫(yī)學;2012年03期

10 張潔;焦云娟;朱國勇;張紅濤;;磷酸肌醇-3激酶/蛋白激酶B抑制劑對人腦膠質(zhì)瘤細胞株U251增殖的影響[J];新鄉(xiāng)醫(yī)學院學報;2010年06期

相關會議論文 前2條

1 賀華;盧亦成;丁夏;陶幫寶;侯瑛;胡國漢;駱純;陳菊祥;董燕;盛平;徐濤;王以政;蔡嶸;;TRPC6通道蛋白是膠質(zhì)瘤病人放療敏感性及生存期長短的指征蛋白[A];2011中華醫(yī)學會神經(jīng)外科學學術會議論文匯編[C];2011年

2 謝江寧;宋素芬;李香;徐秀泉;;桑黃總三萜的提取及其體外抗腦膠質(zhì)瘤U251活性[A];中華醫(yī)學會急診醫(yī)學分會第十六次全國急診醫(yī)學學術年會論文集[C];2013年

相關博士學位論文 前1條

1 郭品;miR-26a對膠質(zhì)母細胞瘤細胞放療敏感性的影響及其機制研究[D];上海交通大學;2014年

，

本文編號：2485168