【摘要】：目的觀察氫溴酸西酞普蘭預處理對大鼠局灶腦缺血再灌注損傷所致腦梗死體積、海馬CA1區(qū)細胞凋亡及超微結構的影響。方法將90只SD大鼠分為假手術組、缺血再灌注組(模型組)、氫溴酸西酞普蘭預處理高、中、低劑量組(給藥劑量分別為20、10、5mg/kg,每天灌胃給藥1次,持續(xù)7d),末次灌胃2h后采用線栓法制作大腦中動脈缺血2h再灌注模型,再灌注24h后,紅四唑氮(TTC)染色法檢測腦梗死體積,透射電鏡觀察損傷側海馬CA1區(qū)細胞超微結構,TUNEL法檢測損傷側海馬CA1區(qū)細胞凋亡并采用激光共聚焦顯微鏡進行觀察和數據測定。結果經氫溴酸西酞普蘭預處理各劑量組與模型組相比,大鼠腦梗死體積減小,差異有統計學意義(P0.05);海馬CA1區(qū)凋亡細胞表達明顯減弱,差異有統計學意義(P0.05);透射電鏡觀察可見氫溴酸西酞普蘭預處理各劑量組大鼠損傷側腦海馬CA1區(qū)細胞超微結構損傷較模型組明顯減輕;以上改變高劑量預處理組效果更為明顯。結論氫溴酸西酞普蘭預處理可減少腦缺血再灌注損傷所致細胞凋亡和梗死面積,具有腦保護作用。
[Abstract]:Objective to observe the effects of citalopram hydrobromide pretreatment on cerebral infarction volume, apoptosis and ultrastructure in hippocampal CA1 region induced by focal cerebral ischemia-reperfusion injury in rats. Methods 90 SD rats were divided into three groups: sham operation group, ischemia-reperfusion group (model group), high, middle and low dose groups pretreated with citalopram hydrobromide (the dosage was 20 mg 路kg ~ (- 1), 10 mg 路kg ~ (- 1), 5 mg 路kg ~ (- 1), respectively), and the rats were given intragastrically once a day for 7 days. The cerebral infarction volume was measured by red tetrazolium (TTC) staining and the ultrastructure of hippocampal CA1 cells in the injured side was observed by transmission electron microscope after 2 hours of middle cerebral artery ischemia for 2 hours. After 24 hours of reperfusion, the volume of cerebral infarction was measured by red tetrazolium nitrogen staining, and the ultrastructure of hippocampal cells in the injured side was observed by transmission electron microscope. Apoptosis in hippocampal CA1 region of injured side was detected by TUNEL assay and observed and measured by laser confocal microscope. Results compared with the model group, the cerebral infarction volume of each dose group pretreated with citalopram hydrobromide was significantly lower than that of the model group (P 0.05), and the expression of apoptotic cells in hippocampal CA1 region was significantly decreased (P 0.05). Transmission electron microscope observation showed that the ultrastructure damage of hippocampal CA1 cells in each dose group was significantly less than that in the model group, and the effect of the above changes was more obvious in the high dose pretreatment group than that in the model group. Conclusion citalopram hydrobromide pretreatment can reduce apoptosis and infarction area induced by cerebral ischemia-reperfusion injury, and has cerebral protective effect.
【作者單位】： 唐山職業(yè)技術學院;華北理工大學醫(yī)學實驗研究中心;唐山市協和醫(yī)院神經內科;
【基金】：河北省科學技術研究與發(fā)展項目(132777218) 河北省唐山市科學技術研究與發(fā)展項目(12130268b)
【分類號】：R743.3

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