【摘要】：目的探討B(tài)DNF對海馬miRNA-124表達(dá)及齒狀回顆粒細(xì)胞興奮性突觸后電流(s EPSCs)的影響,以明確BDNF對顳葉內(nèi)側(cè)癲癇(MTLE)發(fā)病機(jī)制的影響。方法選取哈爾濱醫(yī)科大學(xué)附屬第一臨床醫(yī)院神經(jīng)外科2008年4月-2010年10月手術(shù)治療的MTLE患者12例。RT-pcr技術(shù)檢測BDNF孵育后MTLE患者海馬組織mir-124表達(dá)、膜片鉗技術(shù)檢測BDNF對海馬顆粒細(xì)胞s EPSCs的影響。結(jié)果 (1)BDNF降低了顳葉癲癇海馬miRNA-124的表達(dá)(P0.01);(2)BDNF增加了顆粒細(xì)胞sEPSCs的頻率和幅度(P0.01)。結(jié)論 BDNF降低了顳葉癲癇海馬mir-124的表達(dá),提高顆粒細(xì)胞sEPSCs的頻率和幅度,從而可能對人類MTLE的發(fā)病起到促進(jìn)作用。
[Abstract]:Aim to investigate the effect of BDNF on the expression of miRNA-124 in hippocampus and the excitatory postsynaptic current (s EPSCs) in granulosa cells of dentate gyrus in order to clarify the effect of BDNF on the pathogenesis of (MTLE) in medial temporal lobe epilepsy. Methods from April 2008 to October 2010, 12 patients with MTLE were selected from the neurosurgery department of the first clinical hospital affiliated to Harbin Medical University. The expression of mir-124 in hippocampus of MTLE patients after incubation with BDNF was detected by RT-pcr technique. The effects of BDNF on s EPSCs of hippocampal granulosa cells were detected by patch clamp technique. Results (1) BDNF decreased the expression of miRNA-124 in hippocampus of temporal lobe epilepsy (P0.01); (2). BDNF increased the frequency and amplitude of sEPSCs in granulosa cells (P0.01). Conclusion BDNF can decrease the expression of mir-124 in hippocampus of temporal lobe epilepsy and increase the frequency and amplitude of sEPSCs in granulosa cells, which may play an important role in the pathogenesis of human MTLE.
【作者單位】： 哈爾濱醫(yī)科大學(xué)附屬第四醫(yī)院神經(jīng)內(nèi)科;牡丹江市婦女兒童醫(yī)院;哈爾濱市第二醫(yī)院;
【基金】：黑龍江省教育廳面上項(xiàng)目(No.12541509)
【分類號】：R742.1
，

本文編號：2450991