【摘要】：目的觀察亞硒酸鈉對谷氨酸(Glu)所致神經(jīng)細(xì)胞損傷的影響,并探討其機(jī)制。方法將HT22神經(jīng)細(xì)胞分成3組,對照組加入細(xì)胞培養(yǎng)液培養(yǎng),Glu組加入6 mmol/L Glu和細(xì)胞培養(yǎng)液共同培養(yǎng),亞硒酸鈉組加入100nmol/L亞硒酸鈉、6 mmol/L Glu和細(xì)胞培養(yǎng)液共同培養(yǎng)。MTT法檢測各組6、10、24 h的細(xì)胞增殖抑制率;倒置顯微鏡觀察細(xì)胞大體形態(tài),透射電鏡觀察細(xì)胞超微結(jié)構(gòu);采用氧自由基(ROS)特異性標(biāo)記探針DHE對各組細(xì)胞進(jìn)行標(biāo)記,用熒光電子顯微鏡觀察DHE表達(dá)。結(jié)果 Glu組培養(yǎng)6 h時(shí)的細(xì)胞增殖抑制率為8.71%±0.009%,10、24h時(shí)細(xì)胞增殖抑制率較6 h時(shí)升高,24 h時(shí)最高;亞硒酸鈉組各時(shí)點(diǎn)細(xì)胞增殖抑制率均較Glu組降低(P均0.05)。倒置顯微鏡下可見Glu組細(xì)胞數(shù)量較對照組減少,細(xì)胞皺縮、形態(tài)不規(guī)則,亞硒酸鈉組細(xì)胞數(shù)量較Glu組數(shù)量增多;透射電鏡下可見Glu組細(xì)胞凋亡數(shù)目較對照組增加,線粒體、內(nèi)質(zhì)網(wǎng)損傷加重,亞硒酸鈉組細(xì)胞凋亡數(shù)量較Glu組減少。熒光電子顯微鏡觀察顯示,Glu組較對照組DHE染色的平均光密度增加,亞硒酸鈉組DHE染色較Glu減輕(P均0.01)。結(jié)論硒能夠減輕Glu對神經(jīng)細(xì)胞的損傷作用,其機(jī)制可能與減少ROS產(chǎn)生有關(guān)。
[Abstract]:Objective to investigate the effect of sodium selenite on neuronal injury induced by glutamate (Glu) and its mechanism. Methods HT22 neurons were divided into three groups: the control group was cultured in cell culture medium, the Glu group was cultured in 6 mmol/L Glu and cell culture medium, and the sodium selenite group was added with 100nmol/L sodium selenite. (6) mmol/L Glu and cell culture medium were co-cultured. MTT assay was used to detect the inhibition rate of cell proliferation in each group for 24 h. The cell morphology was observed by inverted microscope and ultrastructure was observed by transmission electron microscope (TEM), and the expression of DHE was observed by fluorescence electron microscope (FEM) and oxygen free radical (ROS) specific probe DHE was used to label the cells in each group. Results the inhibition rate of cell proliferation in Glu group was 8.71% 鹵0.009% at 6 h and increased at 10h and the highest at 24 h. The inhibition rate of cell proliferation in sodium selenite group was lower than that in Glu group at all time points (P 0.05). Under inverted microscope, the number of cells in Glu group was less than that in control group, cell shrinkage and irregular morphology were observed, and the number of cells in sodium selenite group was higher than that in Glu group. Transmission electron microscope showed that the number of apoptosis in Glu group was higher than that in control group, the damage of mitochondria and endoplasmic reticulum was aggravated, and the number of apoptosis in sodium selenite group was lower than that in Glu group. Fluorescence electron microscopy showed that the average optical density of DHE staining in Glu group was higher than that in control group, while DHE staining in sodium selenite group was less than that in Glu group (P0.01). Conclusion selenium can attenuate the injury of Glu to nerve cells, and its mechanism may be related to the reduction of ROS production.
【作者單位】： 寧夏醫(yī)科大學(xué);銀川市婦幼保健院;陜西省中醫(yī)院;
【基金】：國家自然科學(xué)基金地區(qū)項(xiàng)目(81560501) 寧夏高等學(xué)校科學(xué)研究項(xiàng)目(NGY2015068)
【分類號】：R741

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