鈉鈣交換體1、3參與遠(yuǎn)隔創(chuàng)傷后處理的腦保護(hù)作用
發(fā)布時(shí)間：2018-12-11 15:34
【摘要】：目的：應(yīng)用大鼠局灶性腦缺血再灌注模型研究遠(yuǎn)隔創(chuàng)傷后處理(remote postconditioning of trauma, Tra-Post)對(duì)大鼠腦缺血/再灌注損傷的影響,研究鈉鈣交換體(sodium-calcium exchangers, NCX)在這種后處理方式中的作用。 方法：45只雄性SD大鼠隨機(jī)分成3組(n=15)：假手術(shù)組(Sham組)、缺血再灌注組(I/R組)和適度創(chuàng)傷疼痛6h后處理組(Tra-Post-6h組)。①Sham組：大鼠僅分離頸部血管,不進(jìn)行大腦中動(dòng)脈梗阻。②I/R組：采用MCAO模型建立大鼠局灶性腦缺血再灌注模型,缺血90min,再灌注24h。③Tra-Post-6h組：MCAO建模并缺血90min,大腦中動(dòng)脈再灌注開(kāi)始后的6小時(shí)在大鼠腹正中線作2cm的切口,皮膚至腹膜全層切開(kāi),之后分層縫合。三組大鼠在再灌注后觀察24h,并在再灌注開(kāi)始后的第6和24h對(duì)大鼠行改良的神經(jīng)功能評(píng)分(modified neurological severity score, mNSS);用TTC法測(cè)定腦梗死容積；HE染色法觀察腦組織形態(tài)；Nissl染色法計(jì)數(shù)存活神經(jīng)細(xì)胞數(shù)目；免疫組化及Western Blot的方法觀察及測(cè)定缺血側(cè)大腦皮層半影區(qū)NCX1和NCX3蛋白的表達(dá)。 結(jié)果：①TTC染色：Sham組未見(jiàn)腦梗死；與I/R組比較,Tra-Post-6h組腦梗死容積顯著縮小(P0.05)。②HE染色：Sham組大腦皮層神經(jīng)元形態(tài)正常；與I/R組比較,Tra-Post-6h組腦缺血側(cè)皮層半影區(qū)神經(jīng)細(xì)胞層次較清楚,數(shù)目多且細(xì)胞形態(tài)較完整,間質(zhì)水腫減輕；但是Tra-Post-6h組和I/R組腦缺血半影區(qū)細(xì)胞數(shù)目均較Sham組少。③Nissl染色：Sham組大腦皮層神經(jīng)細(xì)胞數(shù)量正常；Tra-Post-6h組缺血半影區(qū)內(nèi)存活的神經(jīng)細(xì)胞數(shù)量比I/R組顯著增加(P0.05),Tra-Post-6h和I/R組存活的神經(jīng)細(xì)胞數(shù)量均較Sham組顯著減少(P0.05)。④改良的神經(jīng)功能評(píng)分：三組大鼠術(shù)前兩次和術(shù)后兩次評(píng)分在組間不存在顯著差異(P0.05),各組大鼠的術(shù)前和術(shù)后兩次評(píng)分組內(nèi)比較也不存在統(tǒng)計(jì)學(xué)意義(P0.05)。⑤免疫組化及Western Blot結(jié)果：Tra-Post-6h組NCX1和NCX3蛋白在缺血側(cè)大腦皮層半影區(qū)的表達(dá)均顯著低于Sham組及I/R組(P0.05)；I/R組和Sham組之間兩種蛋白的表達(dá)量不存在統(tǒng)計(jì)學(xué)差異(P0.05)。 結(jié)論：遠(yuǎn)隔創(chuàng)傷后處理可以對(duì)缺血再灌注的腦組織產(chǎn)生保護(hù)作用；這種腦保護(hù)作用可能通過(guò)減少NCX1和NCX3蛋白的表達(dá)來(lái)實(shí)現(xiàn)。
[Abstract]:Objective: to study the effects of (remote postconditioning of trauma, Tra-Post on cerebral ischemia / reperfusion injury in rats with focal cerebral ischemia-reperfusion injury and to study the sodium calcium exchanger (sodium-calcium exchangers,). The role of NCX in this post-processing. Methods: Forty-five male SD rats were randomly divided into three groups (n = 15): sham operation group (Sham group), ischemia reperfusion group (I / R group) and moderate traumatic pain treatment group (Tra-Post-6h group). Middle cerebral artery occlusion was not performed in 2I/R group: focal cerebral ischemia-reperfusion model was established with MCAO model in rats, ischemia 90 min, reperfusion 24h.3Tra-Post-6h group: MCAO modeling and ischemia 90 min. The middle cerebral artery (MCA) was cut into the abdominal median line of the rat at 6 hours after reperfusion. The skin was cut to the whole peritoneal layer, and then sutured in layers. The rats in the three groups were observed 24 hours after reperfusion, and the modified neurological function score (modified neurological severity score, mNSS);) was used to measure the volume of cerebral infarction by TTC method, and the morphology of brain tissue was observed by HE staining method at 6 and 24 hours after reperfusion. The number of surviving neurons was counted by Nissl staining and the expression of NCX1 and NCX3 in ischemic cerebral cortex penumbra was observed and measured by immunohistochemical and Western Blot methods. Results: 1TTC staining: no cerebral infarction was found in Sham group, and the volume of cerebral infarction was significantly reduced in Tra-Post-6h group compared with I / R group (P0.05). 2HE staining: the morphology of cortical neurons in Sham group was normal. Compared with the I / R group, the level of neurons in the cerebral ischemic penumbra of Tra-Post-6h group was clear, the number of neurons was more, the morphology of the cells was complete, and the interstitial edema was alleviated. However, the number of cerebral ischemic penumbra cells in Tra-Post-6h group and I / R group was less than that in Sham group. 3Nissl staining: the number of cortical neurons in Sham group was normal. The number of neurons surviving in ischemic penumbra in Tra-Post-6h group was significantly higher than that in I / R group (P0.05). The number of nerve cells survived in Tra-Post-6h and I / R groups was significantly lower than that in Sham group (P0.05). 4 improved neurological function score: there was no significant difference between the three groups in the scores of two times before and after operation (P0.05). There was no significant difference between the two scores before and after operation in each group (P0.05). 5 Immunohistochemical and Western Blot results: expression of NCX1 and NCX3 protein in ischemic cerebral cortex penumbra in Tra-Post-6h group It was significantly lower than that in Sham group and I / R group (P0.05). There was no significant difference in the expression of two proteins between I / R group and Sham group (P0.05). Conclusion: the post-traumatic treatment can protect the brain tissue from ischemia and reperfusion, which may be achieved by reducing the expression of NCX1 and NCX3 proteins.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743
本文編號(hào)：2372796
[Abstract]:Objective: to study the effects of (remote postconditioning of trauma, Tra-Post on cerebral ischemia / reperfusion injury in rats with focal cerebral ischemia-reperfusion injury and to study the sodium calcium exchanger (sodium-calcium exchangers,). The role of NCX in this post-processing. Methods: Forty-five male SD rats were randomly divided into three groups (n = 15): sham operation group (Sham group), ischemia reperfusion group (I / R group) and moderate traumatic pain treatment group (Tra-Post-6h group). Middle cerebral artery occlusion was not performed in 2I/R group: focal cerebral ischemia-reperfusion model was established with MCAO model in rats, ischemia 90 min, reperfusion 24h.3Tra-Post-6h group: MCAO modeling and ischemia 90 min. The middle cerebral artery (MCA) was cut into the abdominal median line of the rat at 6 hours after reperfusion. The skin was cut to the whole peritoneal layer, and then sutured in layers. The rats in the three groups were observed 24 hours after reperfusion, and the modified neurological function score (modified neurological severity score, mNSS);) was used to measure the volume of cerebral infarction by TTC method, and the morphology of brain tissue was observed by HE staining method at 6 and 24 hours after reperfusion. The number of surviving neurons was counted by Nissl staining and the expression of NCX1 and NCX3 in ischemic cerebral cortex penumbra was observed and measured by immunohistochemical and Western Blot methods. Results: 1TTC staining: no cerebral infarction was found in Sham group, and the volume of cerebral infarction was significantly reduced in Tra-Post-6h group compared with I / R group (P0.05). 2HE staining: the morphology of cortical neurons in Sham group was normal. Compared with the I / R group, the level of neurons in the cerebral ischemic penumbra of Tra-Post-6h group was clear, the number of neurons was more, the morphology of the cells was complete, and the interstitial edema was alleviated. However, the number of cerebral ischemic penumbra cells in Tra-Post-6h group and I / R group was less than that in Sham group. 3Nissl staining: the number of cortical neurons in Sham group was normal. The number of neurons surviving in ischemic penumbra in Tra-Post-6h group was significantly higher than that in I / R group (P0.05). The number of nerve cells survived in Tra-Post-6h and I / R groups was significantly lower than that in Sham group (P0.05). 4 improved neurological function score: there was no significant difference between the three groups in the scores of two times before and after operation (P0.05). There was no significant difference between the two scores before and after operation in each group (P0.05). 5 Immunohistochemical and Western Blot results: expression of NCX1 and NCX3 protein in ischemic cerebral cortex penumbra in Tra-Post-6h group It was significantly lower than that in Sham group and I / R group (P0.05). There was no significant difference in the expression of two proteins between I / R group and Sham group (P0.05). Conclusion: the post-traumatic treatment can protect the brain tissue from ischemia and reperfusion, which may be achieved by reducing the expression of NCX1 and NCX3 proteins.
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743
【參考文獻(xiàn)】
相關(guān)期刊論文 前1條
1 Arunotai Siriussawakul;Ahmed Zaky;John D Lang;;Role of nitric oxide in hepatic ischemia-reperfusion injury[J];World Journal of Gastroenterology;2010年48期
，本文編號(hào)：2372796
本文鏈接：http://sikaile.net/yixuelunwen/shenjingyixue/2372796.html
最近更新
教材專著
