2006-2010年陜西省克雅氏病監(jiān)測病例特征分析
發(fā)布時間：2018-11-29 07:48
【摘要】：目的了解陜西省克雅氏病(Creutzfeldt-Jakob disease,CJD)的發(fā)病情況、臨床表現(xiàn)及流行病學(xué)特征。方法對2006-2010年陜西省臨床醫(yī)院報告的42例可疑CJD病例的臨床及流行病學(xué)資料進行分析,收集了42份血液標本、41份腦脊液及1份腦組織樣品,利用免疫印跡(western blot,WB)方法檢測腦組織的異常折疊朊蛋白(prion protein scrapie,Pr PSc)和腦脊液的14-3-3蛋白,提取全血基因組DNA并利用聚合酶鏈式反應(yīng)(polymerase chain reaction,PCR)及測序方法對血液中朊蛋白(Prion protein,PRNP或Pr P)基因進行129位多態(tài)性及基因突變分析。結(jié)果共發(fā)現(xiàn)散發(fā)型CJD臨床診斷病例18例,疑似診斷病例3例,家族型CJD 2例。病例的地理分布和職業(yè)無明顯聚集性;臨床診斷病例發(fā)病平均年齡55.9歲,男女比例1.25∶1;快速進行性癡呆為最常見的首發(fā)癥狀,占全部診斷病例的34.78%。結(jié)論陜西監(jiān)測到的CJD病例以散發(fā)型為主,病例的地理分布、職業(yè)、性別比例以及平均年齡均符合散發(fā)型CJD的分布特點。隨訪有助于CJD正確診斷。
[Abstract]:Objective to investigate the incidence, clinical manifestations and epidemiological characteristics of Creutzfeldt-Jakob disease (Creutzfeldt-Jakob disease,CJD) in Shaanxi Province. Methods the clinical and epidemiological data of 42 suspected CJD cases reported by Shaanxi Provincial Clinical Hospital from 2006 to 2010 were analyzed. 42 blood samples, 41 cerebrospinal fluid samples and 1 brain tissue sample were collected. Western blot (western blot, was used. WB) was used to detect the abnormal folding prion protein (prion protein scrapie,Pr PSc) in brain tissue and 14-3-3 protein in cerebrospinal fluid (CSF). Genomic DNA was extracted from whole blood, and polymerase chain reaction (polymerase chain reaction,) was used. PCR and sequencing methods were used to analyze the polymorphism and mutation of prion protein (Prion protein,PRNP or Pr P) gene) in blood. Results 18 cases of sporadic CJD, 3 cases of suspected cases and 2 cases of familial CJD were found. The average age of clinical diagnosis was 55.9 years old, the ratio of male to female was 1.25: 1. Rapid progressive dementia was the most common initial symptom, accounting for 34.78% of all cases. Conclusion sporadic type is the main type of CJD in Shaanxi Province. The geographical distribution, occupation, sex ratio and average age of the cases accord with the distribution characteristics of sporadic CJD. Follow-up is helpful for the correct diagnosis of CJD.
【作者單位】： 陜西省疾病預(yù)防控制中心病毒病預(yù)防控制所;
【基金】：“十二五”國家科技重大專項(2013ZX10004202-001-002)
【分類號】：R742.9
，
本文編號：2364378
[Abstract]:Objective to investigate the incidence, clinical manifestations and epidemiological characteristics of Creutzfeldt-Jakob disease (Creutzfeldt-Jakob disease,CJD) in Shaanxi Province. Methods the clinical and epidemiological data of 42 suspected CJD cases reported by Shaanxi Provincial Clinical Hospital from 2006 to 2010 were analyzed. 42 blood samples, 41 cerebrospinal fluid samples and 1 brain tissue sample were collected. Western blot (western blot, was used. WB) was used to detect the abnormal folding prion protein (prion protein scrapie,Pr PSc) in brain tissue and 14-3-3 protein in cerebrospinal fluid (CSF). Genomic DNA was extracted from whole blood, and polymerase chain reaction (polymerase chain reaction,) was used. PCR and sequencing methods were used to analyze the polymorphism and mutation of prion protein (Prion protein,PRNP or Pr P) gene) in blood. Results 18 cases of sporadic CJD, 3 cases of suspected cases and 2 cases of familial CJD were found. The average age of clinical diagnosis was 55.9 years old, the ratio of male to female was 1.25: 1. Rapid progressive dementia was the most common initial symptom, accounting for 34.78% of all cases. Conclusion sporadic type is the main type of CJD in Shaanxi Province. The geographical distribution, occupation, sex ratio and average age of the cases accord with the distribution characteristics of sporadic CJD. Follow-up is helpful for the correct diagnosis of CJD.
【作者單位】： 陜西省疾病預(yù)防控制中心病毒病預(yù)防控制所;
【基金】：“十二五”國家科技重大專項(2013ZX10004202-001-002)
【分類號】：R742.9
，
本文編號：2364378
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