【摘要】：目的:通過檢測細(xì)胞周期相關(guān)蛋白D1(Cyclin D1)在肌萎縮側(cè)索硬化癥(ALS)轉(zhuǎn)基因小鼠大腦皮層和海馬中的表達(dá)變化,探討Cyclin D1表達(dá)改變與ALS發(fā)病的關(guān)系。方法:選取成年ALS小鼠和同窩野生型小鼠,于發(fā)病早、中、晚期(95 d,108 d,122 d)取材,應(yīng)用免疫熒光技術(shù)檢測Cyclin D1在大腦皮層和海馬的表達(dá)規(guī)律及與神經(jīng)元和星形膠質(zhì)細(xì)胞的共定位關(guān)系,應(yīng)用RT-PCR檢測Cyclin D1 mRNA表達(dá)情況,應(yīng)用免疫印跡法檢測蛋白表達(dá)量的改變。結(jié)果:ALS小鼠和野生型小鼠大腦皮層和海馬中均可檢測到Cyclin D1陽性細(xì)胞,且與神經(jīng)元共表達(dá)。在發(fā)病早、中、晚期,ALS小鼠大腦皮層中Cyclin D1 mRNA和蛋白表達(dá)較野生型鼠增多(P0.05,P0.01,P0.001);與同窩野生型小鼠相比,ALS小鼠海馬中Cyclin D1 mRNA和蛋白在發(fā)病的早、中、晚期表達(dá)均降低(P0.05,P0.01,P0.001)。Cyclin D1陽性細(xì)胞主要分布在海馬CA區(qū)(包括CA1、CA2和CA3),DG區(qū)僅散在表達(dá)。結(jié)論:Cyclin D1在ALS轉(zhuǎn)基因小鼠大腦皮層和海馬中表達(dá)異常,表明Cyclin D1調(diào)節(jié)的細(xì)胞周期改變與ALS大腦皮層和海馬區(qū)病變密切相關(guān)。
[Abstract]:Aim: to investigate the expression of cell cycle associated protein D1 (Cyclin D1) in cerebral cortex and hippocampus of (ALS) transgenic mice with amyotrophic lateral sclerosis and to explore the relationship between the expression of Cyclin D1 and the pathogenesis of ALS. Methods: adult ALS mice and wild-type mice of the same litter were selected and collected at the early, middle and late stage of onset (95 d ~ 108 d ~ 122d). The expression of Cyclin D1 in cerebral cortex and hippocampus and its co-localization with neurons and astrocytes were detected by immunofluorescence technique. The expression of Cyclin D1 mRNA was detected by RT-PCR and the change of protein expression by Western blot. Results: Cyclin D1 positive cells were detected in cortex and hippocampus of ALS mice and wild-type mice. In the early, middle and late stage of onset, the expression of Cyclin D1 mRNA and protein in cerebral cortex of ALS mice was higher than that of wild-type mice (P0.05, P0.01, P0.001). Compared with wild-type mice, the expression of Cyclin D1 mRNA and protein in hippocampus of ALS mice decreased in the early, middle and late stages (P0.05P0.01P0.01P0.001). Cyclin D1 positive cells mainly distributed in the CA region of hippocampus (including CA1,CA2 and CA3). The DG region was only scattered in expression. Conclusion: the abnormal expression of Cyclin D1 in cortex and hippocampus of ALS transgenic mice suggests that the changes of cell cycle regulated by Cyclin D1 are closely related to the pathological changes of ALS cortex and hippocampus.
【作者單位】： 濰坊醫(yī)學(xué)院附屬醫(yī)院;濰坊醫(yī)學(xué)院;濰坊醫(yī)學(xué)院組織學(xué)與胚胎學(xué)教研室;
【基金】：國家自然科學(xué)基金(81401066) 山東省自然科學(xué)基金(ZR2012HQ021,ZR2016HM60) 山東省教育廳課題(J11LF16) 山東省醫(yī)藥衛(wèi)生科技發(fā)展計(jì)劃項(xiàng)目(2016WS0691,2016WS0666) 濰坊醫(yī)學(xué)院大學(xué)生科技創(chuàng)新基金(KX2016009,KX2016015)
【分類號】：R744.8

【相似文獻(xiàn)】

相關(guān)期刊論文 前2條

1 ;Effect of Cell Cycle Inhibitor Olomoucine on Astroglial Proliferation and Scar Formation after Focal Cerebral Infarction in Rats[J];神經(jīng)損傷與功能重建;2011年05期

2 陳彬;王偉;;The Expression of Cyclins in Neurons of Rats after Focal Cerebral Ischemia[J];Journal of Huazhong University of Science and Technology(Medical Sciences);2008年01期

，

本文編號：2351708