【摘要】：目的:探索成纖維細(xì)胞生長(zhǎng)因子10(fibroblast growth factor 10,FGF10)對(duì)脂多糖(lipopolysaccharide,LPS)刺激下小膠質(zhì)細(xì)胞BV2活化的影響。方法:小鼠BV2小膠質(zhì)細(xì)胞用細(xì)胞培養(yǎng)基DMEM培養(yǎng),置于37℃、5%CO_2、飽和濕度的培養(yǎng)箱中培養(yǎng),1~2 d換液,4~5 d傳代。實(shí)驗(yàn)分為對(duì)照組、LPS組和FGF10組,FGF10組的BV2細(xì)胞預(yù)先給予FGF10 1 mg/L 30 min后,在LPS組和FGF10組中加入500 mg/L的LPS,在不同時(shí)點(diǎn)進(jìn)行檢測(cè)。用倒置顯微鏡觀察小膠質(zhì)細(xì)胞的形態(tài)學(xué)改變,RT-qPCR和ELISA分別檢測(cè)腫瘤壞死因子α(TNF-α)轉(zhuǎn)錄和蛋白表達(dá)水平的改變來觀測(cè)BV2細(xì)胞的活化情況。結(jié)果:靜息狀態(tài)下BV2細(xì)胞形態(tài)呈圓形或橢圓形,經(jīng)過24 h LPS刺激后,BV2細(xì)胞形狀向多極或紡錘樣改變,活化細(xì)胞數(shù)量比值明顯高于對(duì)照組;預(yù)先給予FGF10能抑制LPS刺激下的BV2細(xì)胞向活化形態(tài)改變,活化的BV2細(xì)胞明顯減少。給予LPS刺激6 h后,LPS組TNF-α的mRNA水平相比于對(duì)照組顯著升高,然而預(yù)先給予FGF10會(huì)顯著抑制TNF-α的轉(zhuǎn)錄。LPS作用24 h后,細(xì)胞培養(yǎng)上清液內(nèi)TNF-α的表達(dá)水平與對(duì)照組相比顯著上升,而預(yù)先給予FGF10在蛋白水平顯著抑制TNF-α的表達(dá)。結(jié)論:FGF10能夠成功抑制LPS刺激下BV2細(xì)胞的活化,有望成為治療經(jīng)膠質(zhì)細(xì)胞介導(dǎo)的神經(jīng)系統(tǒng)炎癥性疾病的一種有效藥物。
[Abstract]:Aim: to investigate the effect of fibroblast growth factor 10 (fibroblast growth factor 10 FGF10 on the activation of BV2 in microglia induced by lipopolysaccharide (lipopolysaccharide,LPS). Methods: mouse BV2 microglia cells were cultured on DMEM medium and cultured at 37 鈩，

本文編號(hào)：2330068