NETs在癲癇患者癲癇發(fā)作后血漿、腦脊液中表達(dá)變化研究
發(fā)布時間：2018-11-08 09:22
【摘要】：目的白細(xì)胞向腦內(nèi)浸潤引起炎癥反應(yīng)在癲癇的發(fā)生和發(fā)展過程中起著極其重要的作用。然而,癲癇發(fā)作后白細(xì)胞引起神經(jīng)元損傷的具體機(jī)制尚不清楚。中性粒細(xì)胞胞外誘捕網(wǎng)(Neutrophil extracellular traps,NETs)是由中性粒細(xì)胞釋放到胞外的包含有DNA、組蛋白、髓過氧化物酶等抗菌蛋白的纖維網(wǎng)狀結(jié)構(gòu),在感染性疾病中扮演著重要的角色,并能加重神經(jīng)元損害。本研究觀察全面強(qiáng)直陣攣性發(fā)作(Generalized tonic-clonic seizures,GTCS)癲癇患者發(fā)作后,腦脊液(cerebrospinal fluid,CSF)和血漿(Plasma)中NETs(dsDNA、MPO)含量及血漿中S100B水平,并分析NETs與S100B的相關(guān)性,探討NETs與血腦屏障通透性關(guān)系,為探索癲癇治療的新靶點提供理論依據(jù)。方法選取2015年11月至2016年12月入住西安醫(yī)學(xué)院第一附屬醫(yī)院神經(jīng)內(nèi)科的GTCS癲癇患者31例及緊張性頭痛患者27例,并于發(fā)病24h內(nèi)進(jìn)行血漿、腦脊液標(biāo)本的采集。采用PicoGreen dsDNA Quantitation kits和酶聯(lián)免疫吸附法(enzyme-linked immunosorbent assay,ELISA)分別測定癲癇組與對照組血漿及腦脊液中NETs(dsDNA、MPO)和S100B含量,并分析二者之間的相關(guān)性。符合正態(tài)分布的計量資料用x±s表示,不符合正態(tài)分布的計量資料用M(LQ,UQ)表示,兩組獨立樣本比較采用T檢驗(符合正態(tài)分布、方差齊的計量資料)和Mann-Whitney U檢驗(不符合正態(tài)分布、方差不齊的計量資料),兩組相關(guān)數(shù)據(jù)間比較采用Wilcoxon帶符號秩檢驗;性別構(gòu)成比等分類變量資料采用?2檢驗,數(shù)據(jù)相關(guān)性分析采用Spearman秩相關(guān)系數(shù)檢驗。所有統(tǒng)計學(xué)分析中,(49)(27)0.05認(rèn)為差異有統(tǒng)計學(xué)意義。結(jié)果癲癇組血漿、腦脊液中dsDNA的含量明顯高于對照組((49)(27)0.001;(49)(27)0.05),血漿、腦脊液中MPO的含量高于對照組((49)(27)0.05);癲癇組S100B水平明顯高于對照組((49)(27)0.05);癲癇組腦脊液/血漿中dsDNA和MPO水平與血漿S100B含量均不具有相關(guān)性((49)0.05)。結(jié)論GTCS癲癇患者發(fā)作后血漿和腦脊液中NETs(dsDNA、MPO)濃度均升高,說明NETs(dsDNA、MPO)可能參與了癲癇的發(fā)生與發(fā)展。GTCS癲癇患者血漿中S100B水平較對照組明顯升高,證實了癲癇發(fā)作與血腦屏障破壞有關(guān)。GTCS癲癇患者腦脊液、血漿中NETs(dsDNA、MPO)含量與血漿S100B無相關(guān)性,提示NETs的形成與GTCS癲癇患者發(fā)作后血腦屏障破壞可能無關(guān)。
[Abstract]:Objective inflammation induced by leukocyte infiltration into the brain plays an important role in the occurrence and development of epilepsy. However, the mechanism of leukocyte-induced neuronal damage after seizure is unclear. Neutrophil extracellular entrapment (Neutrophil extracellular traps,NETs) is a fibrous reticular structure containing DNA, histone, myeloperoxidase and other antibacterial proteins released from neutrophil to extracellular, which plays an important role in infectious diseases. And can aggravate neuronal damage. In this study, the contents of NETs (dsDNA,MPO) in cerebrospinal fluid (cerebrospinal fluid,CSF) and plasma (Plasma) and the level of S100B in plasma were observed in patients with generalized tonic-clonic seizure (Generalized tonic-clonic seizures,GTCS) after seizure, and the correlation between NETs and S100B was analyzed. To explore the relationship between NETs and blood-brain barrier permeability, and to provide theoretical basis for exploring new targets of epilepsy therapy. Methods from November 2015 to December 2016, 31 patients with GTCS epilepsy and 27 patients with tension headache were enrolled in the Department of Neurology, first affiliated Hospital of Xi'an Medical College. The samples of plasma and cerebrospinal fluid (CSF) were collected within 24 hours after onset. The contents of NETs (dsDNA,MPO) and S100B in plasma and cerebrospinal fluid of epileptic group and control group were determined by PicoGreen dsDNA Quantitation kits and enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay,ELISA), and the correlation between them was analyzed. The metrological data in accordance with normal distribution are expressed in x 鹵s, and those that do not conform to normal distribution are expressed in M (LQ,UQ). The two groups of independent samples are compared by T test (consistent with normal distribution). Mann-Whitney U test (not in accordance with the normal distribution, the measurement data with uneven variance) and the Wilcoxon signed rank test were used in the comparison between the two groups of related data. The data of sex composition ratio and other classified variables were tested by? 2 test, and Spearman rank correlation coefficient test was used for data correlation analysis. In all statistical analysis, (49) (27) 0.05 thought the difference was statistically significant. Results the levels of dsDNA in plasma and cerebrospinal fluid in epilepsy group were significantly higher than those in control group (49) (27) and (49) (27). The contents of MPO in plasma and cerebrospinal fluid were higher than those in control group (49) (27). The level of S100B in epileptic group was significantly higher than that in control group (49) (27). There was no correlation between the levels of dsDNA and MPO in cerebrospinal fluid / plasma and the content of S100B in epileptic group (49) 0.05). Conclusion the concentration of NETs (dsDNA,MPO) in plasma and cerebrospinal fluid (CSF) of GTCS epileptic patients increased after seizure, indicating that NETs (dsDNA,MPO) may be involved in the occurrence and development of epilepsy. The level of S100B in GTCS epileptic patients was significantly higher than that in control group. There was no correlation between the content of NETs (dsDNA,MPO) in plasma and S100B in patients with GTCS epilepsy, suggesting that the formation of NETs may not be related to the breakdown of blood-brain barrier in GTCS epileptic patients.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R742.1
本文編號：2318056
[Abstract]:Objective inflammation induced by leukocyte infiltration into the brain plays an important role in the occurrence and development of epilepsy. However, the mechanism of leukocyte-induced neuronal damage after seizure is unclear. Neutrophil extracellular entrapment (Neutrophil extracellular traps,NETs) is a fibrous reticular structure containing DNA, histone, myeloperoxidase and other antibacterial proteins released from neutrophil to extracellular, which plays an important role in infectious diseases. And can aggravate neuronal damage. In this study, the contents of NETs (dsDNA,MPO) in cerebrospinal fluid (cerebrospinal fluid,CSF) and plasma (Plasma) and the level of S100B in plasma were observed in patients with generalized tonic-clonic seizure (Generalized tonic-clonic seizures,GTCS) after seizure, and the correlation between NETs and S100B was analyzed. To explore the relationship between NETs and blood-brain barrier permeability, and to provide theoretical basis for exploring new targets of epilepsy therapy. Methods from November 2015 to December 2016, 31 patients with GTCS epilepsy and 27 patients with tension headache were enrolled in the Department of Neurology, first affiliated Hospital of Xi'an Medical College. The samples of plasma and cerebrospinal fluid (CSF) were collected within 24 hours after onset. The contents of NETs (dsDNA,MPO) and S100B in plasma and cerebrospinal fluid of epileptic group and control group were determined by PicoGreen dsDNA Quantitation kits and enzyme linked immunosorbent assay (enzyme-linked immunosorbent assay,ELISA), and the correlation between them was analyzed. The metrological data in accordance with normal distribution are expressed in x 鹵s, and those that do not conform to normal distribution are expressed in M (LQ,UQ). The two groups of independent samples are compared by T test (consistent with normal distribution). Mann-Whitney U test (not in accordance with the normal distribution, the measurement data with uneven variance) and the Wilcoxon signed rank test were used in the comparison between the two groups of related data. The data of sex composition ratio and other classified variables were tested by? 2 test, and Spearman rank correlation coefficient test was used for data correlation analysis. In all statistical analysis, (49) (27) 0.05 thought the difference was statistically significant. Results the levels of dsDNA in plasma and cerebrospinal fluid in epilepsy group were significantly higher than those in control group (49) (27) and (49) (27). The contents of MPO in plasma and cerebrospinal fluid were higher than those in control group (49) (27). The level of S100B in epileptic group was significantly higher than that in control group (49) (27). There was no correlation between the levels of dsDNA and MPO in cerebrospinal fluid / plasma and the content of S100B in epileptic group (49) 0.05). Conclusion the concentration of NETs (dsDNA,MPO) in plasma and cerebrospinal fluid (CSF) of GTCS epileptic patients increased after seizure, indicating that NETs (dsDNA,MPO) may be involved in the occurrence and development of epilepsy. The level of S100B in GTCS epileptic patients was significantly higher than that in control group. There was no correlation between the content of NETs (dsDNA,MPO) in plasma and S100B in patients with GTCS epilepsy, suggesting that the formation of NETs may not be related to the breakdown of blood-brain barrier in GTCS epileptic patients.
【學(xué)位授予單位】：寧夏醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R742.1
【參考文獻(xiàn)】
相關(guān)期刊論文 前4條
1 尹曉雪;王琪;高飛;金美麗;劉彥虹;;中性粒細(xì)胞胞外誘捕網(wǎng)在非感染性炎性疾病中的作用[J];免疫學(xué)雜志;2015年06期
2 黃鑫;高雪靜;王淵;張瑩;唐莎;鄒麗云;王莉;張靜波;;分子伴侶性自噬參與LAMP-2抗體誘導(dǎo)中性粒細(xì)胞胞外捕網(wǎng)形成[J];免疫學(xué)雜志;2015年03期
3 周堅;張亞衡;周劍濤;;抗中性粒細(xì)胞胞外誘捕網(wǎng)的細(xì)菌毒力因子[J];分子診斷與治療雜志;2011年02期
4 ;解讀國際抗癲癇聯(lián)盟和癲癇局癲癇及癲癇發(fā)作新定義[J];中華醫(yī)學(xué)雜志;2007年29期
，本文編號：2318056
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