神經(jīng)膠質(zhì)瘤與博爾納病病毒感染相關(guān)性研究
發(fā)布時間：2018-11-02 18:59
【摘要】：目的:研究貴州省遵義地區(qū)神經(jīng)膠質(zhì)瘤患者中是否存在博爾納病病毒(BDV)感染;探討B(tài)DV P40基因片段的特點,以及BDV陽性神經(jīng)膠質(zhì)瘤患者的臨床表現(xiàn)特點。方法:收集遵義醫(yī)學(xué)院附屬醫(yī)院神經(jīng)外科2015年10月至2016年09月行神經(jīng)膠質(zhì)瘤切除術(shù)患者腫瘤組織、外周血樣本各22例,同期重度顱腦外傷行顱內(nèi)減壓術(shù)患者腦組織級體檢中心健康體檢者外周血樣本24例;采用實時熒光定量反轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)法分別檢測22例神經(jīng)膠質(zhì)瘤患者術(shù)后腫瘤組織及其外周血淋巴細(xì)胞(PBL)、24例腦外傷顱內(nèi)減壓術(shù)后腦組織、24例健康體檢者PBL中的BDV P40基因片段,陽性標(biāo)本進(jìn)行基因測序及分析,總結(jié)BDV P40陽性者臨床表現(xiàn)特點。結(jié)果:神經(jīng)膠質(zhì)瘤組腫瘤組織中BDV P40的陽性率為18.2%,顱內(nèi)減壓術(shù)組腦組織中BDV P40的陽性率為0%,兩組相比差異具有統(tǒng)計學(xué)意義(P0.05);神經(jīng)膠質(zhì)瘤組PBL中BDV P40的陽性率為9.1%,健康體檢者組PBL中BDV P40的陽性率為0%,兩組相比差異不具有統(tǒng)計學(xué)意義(P0.05);其中,有2例膠質(zhì)瘤患者腫瘤組織及PBL均檢測出BDV P40基因片段,提示腦組織及PBL中的BDV P40檢測結(jié)果不完全對等,且腫瘤組織中該病毒基因片段檢出率更高。本實驗擴(kuò)增的目的基因片段為BDV P40(nt242-320),與標(biāo)準(zhǔn)株Strain V和流行株He/80相比同源性分別為為97.2%、100%;較Strain V對比,3個堿基發(fā)生了點突變(nt262GC、nt303 CT及nt313 AG),突變率為3.8%;與He/80對比,2個堿基發(fā)生了點突變(262 nt262GC、nt313AG),突變率為2.5%。BDV P40陽性患者臨床表現(xiàn)以頭痛、頭昏為主,符合顱內(nèi)腫瘤患者臨床表現(xiàn),不具有差異性(P0.05)。結(jié)論:貴州遵義地區(qū)神經(jīng)膠質(zhì)瘤患者中存在BDV感染,BDV感染可能與神經(jīng)膠質(zhì)瘤的發(fā)生相關(guān);該地區(qū)BDV P40基因片段保守、穩(wěn)定,可作為檢測BDV感染的指標(biāo)之一;BDV P40陽性的神經(jīng)膠質(zhì)瘤患者臨床表現(xiàn)與BDV P40陰性的神經(jīng)膠質(zhì)瘤患者臨床表現(xiàn)無差異性。
[Abstract]:Objective: to study the presence of Borna disease virus (BDV) infection in glioma patients in Zunyi, Guizhou province, and to investigate the characteristics of BDV P40 gene fragment and the clinical manifestations of BDV positive glioma patients. Methods: tumor tissues were collected from neurosurgery department of affiliated hospital of Zunyi Medical College from October 2015 to September 2016. The peripheral blood samples of 24 patients with severe craniocerebral trauma undergoing craniocerebral decompression were studied. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect tumor tissues and peripheral blood lymphocytes (PBL),) in 22 patients with glioma after intracranial decompression. The BDV P40 gene fragment in PBL of 24 healthy persons was sequenced and analyzed, and the clinical features of BDV P40 positive patients were summarized. Results: the positive rate of BDV P40 was 18.2g in glioma group and 0 in intracranial decompression group (P0.05). The positive rate of BDV P40 in PBL was 9.1 in glioma group and 0 in PBL in healthy control group. There was no significant difference between the two groups (P0.05). BDV P40 gene fragments were detected in tumor tissues and PBL of two patients with glioma, indicating that the detection results of BDV P40 in brain tissues and PBL were not identical, and the detection rate of P40 gene fragments in tumor tissues was higher. The target gene fragment was BDV P40 (nt242-320). Compared with the standard strain Strain V and the epidemic strain He/80, the homology of the target gene fragment was 97.2and 100, respectively. Compared with Strain V, point mutation occurred in three bases (nt262GC,nt303 CT and nt313 AG), mutation rate was 3.8%). Compared with He/80, two bases had point mutation (262 nt262GC,nt313AG), and the mutation rate was headache and dizziness in patients with positive 2.5%.BDV P40, which was consistent with the clinical manifestations of intracranial tumor patients, but had no difference (P0.05). Conclusion: there is BDV infection in patients with glioma in Zunyi, Guizhou province, and BDV infection may be related to the occurrence of glioma, the BDV P40 gene fragment in this area is conservative and stable, and can be used as an index for detecting BDV infection. There was no difference between BDV P40 positive gliomas and BDV P40 negative gliomas.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.41
[Abstract]:Objective: to study the presence of Borna disease virus (BDV) infection in glioma patients in Zunyi, Guizhou province, and to investigate the characteristics of BDV P40 gene fragment and the clinical manifestations of BDV positive glioma patients. Methods: tumor tissues were collected from neurosurgery department of affiliated hospital of Zunyi Medical College from October 2015 to September 2016. The peripheral blood samples of 24 patients with severe craniocerebral trauma undergoing craniocerebral decompression were studied. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect tumor tissues and peripheral blood lymphocytes (PBL),) in 22 patients with glioma after intracranial decompression. The BDV P40 gene fragment in PBL of 24 healthy persons was sequenced and analyzed, and the clinical features of BDV P40 positive patients were summarized. Results: the positive rate of BDV P40 was 18.2g in glioma group and 0 in intracranial decompression group (P0.05). The positive rate of BDV P40 in PBL was 9.1 in glioma group and 0 in PBL in healthy control group. There was no significant difference between the two groups (P0.05). BDV P40 gene fragments were detected in tumor tissues and PBL of two patients with glioma, indicating that the detection results of BDV P40 in brain tissues and PBL were not identical, and the detection rate of P40 gene fragments in tumor tissues was higher. The target gene fragment was BDV P40 (nt242-320). Compared with the standard strain Strain V and the epidemic strain He/80, the homology of the target gene fragment was 97.2and 100, respectively. Compared with Strain V, point mutation occurred in three bases (nt262GC,nt303 CT and nt313 AG), mutation rate was 3.8%). Compared with He/80, two bases had point mutation (262 nt262GC,nt313AG), and the mutation rate was headache and dizziness in patients with positive 2.5%.BDV P40, which was consistent with the clinical manifestations of intracranial tumor patients, but had no difference (P0.05). Conclusion: there is BDV infection in patients with glioma in Zunyi, Guizhou province, and BDV infection may be related to the occurrence of glioma, the BDV P40 gene fragment in this area is conservative and stable, and can be used as an index for detecting BDV infection. There was no difference between BDV P40 positive gliomas and BDV P40 negative gliomas.
【學(xué)位授予單位】：遵義醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.41
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