發(fā)布時間：2018-10-17 18:30

【摘要】：目的:通過觀察保留性神經(jīng)損傷(SNI)大鼠模型中Nav1.8在受損背根神經(jīng)節(jié)(dorsal root ganglion,DRG)、外周軸突以及表皮神經(jīng)纖維中的表達(dá)變化,探討Nav1.8蛋白在部分神經(jīng)損傷所致神經(jīng)病理性疼痛中的可能機(jī)制。方法:選擇清潔級SD大鼠12只,隨機(jī)分為兩組:保留性神經(jīng)損傷組(SNI組)與假手術(shù)組(Sham組);造模時分別在SNI組和Sham組大鼠腓腸神經(jīng)鞘內(nèi)注射熒光金(FG),造模當(dāng)天和造模后第7天與第14天測量兩組大鼠機(jī)械刺激傷害感受閾值(MWT)。術(shù)后14天處死SNI組和Sham組大鼠,取L5 DRG、腓腸神經(jīng)、脛神經(jīng)、腓總神經(jīng)組織,以及腓腸神經(jīng)和脛神經(jīng)支配區(qū)域皮膚組織。采用免疫熒光技術(shù)檢測兩組大鼠Nav1.8在DRG FG陽性(FG+)區(qū)域和陰性(FG-)區(qū)域,腓腸神經(jīng)、脛神經(jīng)、腓總神經(jīng),以及腓腸神經(jīng)和脛神經(jīng)支配皮膚區(qū)域的表達(dá)變化。結(jié)果:L5 DRG中FG+區(qū)域與FG-區(qū)域呈區(qū)域分布。術(shù)后第7天和第14天,SNI組大鼠的MWT值較Sham組明顯降低(P0.01)。Nav1.8在SNI組大鼠腓腸神經(jīng)、脛神經(jīng)、腓總神經(jīng)以及腓腸神經(jīng)支配皮膚區(qū)域的表達(dá)顯著高于Sham組(P0.05),而Nav1.8在脛神經(jīng)支配皮膚區(qū)域的表達(dá)明顯低于Sham組(P0.05)。Nav1.8在SNI組大鼠DRG FG+和FG-區(qū)域的表達(dá)高于Sham組(P0.05),而在SNI組內(nèi),DRG FG+區(qū)域的Nav1.8表達(dá)顯著高于FG-區(qū)域(P0.05)。結(jié)論:DRG中腓腸神經(jīng)投射區(qū)域與脛神經(jīng)、腓總神經(jīng)投射區(qū)域呈解剖分離狀態(tài)。Nav1.8在DRG、未損傷神經(jīng)及支配皮膚表達(dá)的增高可能是部份神經(jīng)損傷引起慢性神經(jīng)病理性疼痛的致病因素之一。
[Abstract]:Objective: to observe the expression of Nav1.8 in the injured dorsal root ganglion (dorsal root ganglion,DRG), peripheral axons and epidermal nerve fibers in (SNI) rats. To explore the possible mechanism of Nav1.8 protein in neuropathic pain caused by partial nerve injury. Methods: twelve clean grade SD rats were selected. Two groups were randomly divided into two groups: the SNI group (SNI group) and the sham operation group (Sham group), and the rats in SNI group and Sham group were given intrathecal injection of fluorescent gold (FG), on the same day and on the 7th and 14th days after modeling. (MWT). Of nociceptive threshold of mechanical stimulation in rats On the 14th day after operation, the rats in SNI group and Sham group were killed. The sural nerve, tibial nerve, common peroneal nerve, sural nerve and tibial nerve innervated area skin tissue of L 5 DRG, were taken out. Immunofluorescence technique was used to detect the expression of Nav1.8 in the areas of DRG FG positive (FG) and FG-, sural nerve, tibial nerve, common peroneal nerve, sural nerve and tibial nerve innervated skin. Results: the region of FG and FG- were distributed in L 5 DRG. On the 7th and 14th day after operation, the MWT value of SNI group was significantly lower than that of Sham group (P0.01). Nav1.8 was observed in sural nerve and tibial nerve in SNI group. The expression of common peroneal nerve and sural nerve innervated skin area was significantly higher than that of Sham group (P0.05), while the expression of Nav1.8 in tibial nerve innervation skin area was significantly lower than that in Sham group (P0.05). The expression of Nav1.8 in DRG FG and FG- region of SNI group was higher than that of Sham group (P0.05), while in SNI group, the expression of Nav1.8 was higher in SNI group than in Sham group (P0.05). The expression of Nav1.8 in, DRG FG was significantly higher than that in FG- (P0.05). Conclusion: the area of sural nerve projection and tibial nerve in DRG, The increased expression of Nav1.8 in the uninjured and innervated skin of DRG, may be one of the pathogenic factors of chronic neuropathic pain caused by partial nerve injury.
【學(xué)位授予單位】：川北醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R741

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