缺血再灌注損傷大鼠患側(cè)骨骼肌形態(tài)學(xué)及Atrogin-1、MuRF-1mRNA表達(dá)的變化
發(fā)布時(shí)間：2018-09-04 13:57
【摘要】：目的通過(guò)制作腦缺血再灌注損傷大鼠模型,觀察大鼠腦缺血再灌住損傷早期患側(cè)骨骼形態(tài)學(xué)變化及Atrogin-1和MuRF-1mRNA表達(dá)水平的變化。探討腦缺血再灌注損傷大鼠患側(cè)骨骼肌早期萎縮的發(fā)生機(jī)制。 方法60只雄性wistar大鼠,10只為假手術(shù)對(duì)照組(A組),其余50只采用Longa線栓法建立大鼠大腦中動(dòng)脈栓塞模型(MCAO),其中30只造模成功大鼠隨機(jī)分為B、C、D組,每組10只。B組為造模成功后1天組, C組為造模成功后4天組, D組為造模成功后7天組。應(yīng)用Bederson評(píng)分評(píng)價(jià)動(dòng)物的神經(jīng)損傷后的恢復(fù)情況；分別獲取A、B、C、D四組右側(cè)肱二頭肌,對(duì)各組通過(guò)HE染色檢測(cè)肌纖維橫截面積；通過(guò)熒光定量RT-PCR觀察大鼠患側(cè)骨骼肌中atrogin-1和MuRF-1mRNA表達(dá)的變化。 結(jié)果B、C、D組大鼠Bederson評(píng)分明顯高于對(duì)照組A組(p0.05)；B、C、D組大鼠間Bederson評(píng)分無(wú)統(tǒng)計(jì)學(xué)差異(p0.05)。患側(cè)骨骼肌HE染色顯示A、B、C三組肌纖維橫截面積兩兩比較,差異無(wú)統(tǒng)計(jì)學(xué)意義(p0.05)；D組大鼠患側(cè)骨骼肌肌纖維橫截面積分別與其余三組相比,差異均有統(tǒng)計(jì)學(xué)意義(p0.05)。B、C、D組大鼠患側(cè)Atrogin-1和MuRF-1mRNA的表達(dá)水平分別與對(duì)照組相比,差異均有統(tǒng)計(jì)學(xué)意義(p0.05)；B組與C組相比較無(wú)顯著差異(p0.05)；D組大鼠分別與B、C組相比,差異均有統(tǒng)計(jì)學(xué)意義(p0.05)。 結(jié)論腦缺血再灌注損傷大鼠早期患側(cè)骨骼肌形態(tài)學(xué)可能已經(jīng)發(fā)生變化,Atrogin-1和MuRF-1mRNA在腦缺血再灌注損傷大鼠早期骨骼肌中表達(dá),腦缺血再灌注損傷大鼠早期骨骼肌中泛素連接酶蛋白降解通路可能已被激活。
[Abstract]:Objective to observe the changes of bone morphology and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats. To investigate the mechanism of early atrophy of skeletal muscle in rats with cerebral ischemia reperfusion injury. Methods 60 male wistar rats (n = 10) were used as sham-operated control group (group A). The other 50 rats were randomly divided into two groups: group A (n = 30), (MCAO), model of middle cerebral artery embolization (MCAO) in rats were established by Longa thread embolization, and 30 rats were randomly divided into two groups: group B (n = 30). There were 10 rats in each group. Group B was one day after successful modeling, group C was 4 days after successful modeling, and group D was 7 days after successful modeling. Bederson score was used to evaluate the recovery of nerve injury in animals, the right biceps brachii muscles of four groups were obtained respectively, and the cross sectional area of muscle fibers was detected by HE staining in each group. The changes of atrogin-1 and MuRF-1mRNA expression in skeletal muscle of the affected side of rats were observed by fluorescence quantitative RT-PCR. Results there was no significant difference in the Bederson score between the two groups compared with the control group A (p0.05) and the control group (p0.05). There was no significant difference in the Bederson score between the two groups (p0.05). HE staining of the affected skeletal muscle showed that the cross sectional area of muscle fibers in the three groups was not significantly different from that in the other three groups, and there was no significant difference in the cross sectional area of skeletal muscle between the D group and the other three groups. The expression levels of Atrogin-1 and MuRF-1mRNA in the affected side of rats in group B were significantly higher than those in group C (p0.05). There was no significant difference between group B and group C (p0.05), and there was no significant difference between group D and group C in the expression of Atrogin-1 and MuRF-1mRNA. The difference was statistically significant (p0.05). Conclusion the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury may have been changed in the skeletal muscle of rats with cerebral ischemia-reperfusion injury, and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats may have been changed. The proteolysis pathway of ubiquitin ligase in skeletal muscle may have been activated in the early stage of cerebral ischemia reperfusion injury in rats.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743.3
本文編號(hào)：2222308
[Abstract]:Objective to observe the changes of bone morphology and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats. To investigate the mechanism of early atrophy of skeletal muscle in rats with cerebral ischemia reperfusion injury. Methods 60 male wistar rats (n = 10) were used as sham-operated control group (group A). The other 50 rats were randomly divided into two groups: group A (n = 30), (MCAO), model of middle cerebral artery embolization (MCAO) in rats were established by Longa thread embolization, and 30 rats were randomly divided into two groups: group B (n = 30). There were 10 rats in each group. Group B was one day after successful modeling, group C was 4 days after successful modeling, and group D was 7 days after successful modeling. Bederson score was used to evaluate the recovery of nerve injury in animals, the right biceps brachii muscles of four groups were obtained respectively, and the cross sectional area of muscle fibers was detected by HE staining in each group. The changes of atrogin-1 and MuRF-1mRNA expression in skeletal muscle of the affected side of rats were observed by fluorescence quantitative RT-PCR. Results there was no significant difference in the Bederson score between the two groups compared with the control group A (p0.05) and the control group (p0.05). There was no significant difference in the Bederson score between the two groups (p0.05). HE staining of the affected skeletal muscle showed that the cross sectional area of muscle fibers in the three groups was not significantly different from that in the other three groups, and there was no significant difference in the cross sectional area of skeletal muscle between the D group and the other three groups. The expression levels of Atrogin-1 and MuRF-1mRNA in the affected side of rats in group B were significantly higher than those in group C (p0.05). There was no significant difference between group B and group C (p0.05), and there was no significant difference between group D and group C in the expression of Atrogin-1 and MuRF-1mRNA. The difference was statistically significant (p0.05). Conclusion the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury may have been changed in the skeletal muscle of rats with cerebral ischemia-reperfusion injury, and the expression of Atrogin-1 and MuRF-1mRNA in the early stage of cerebral ischemia-reperfusion injury in rats may have been changed. The proteolysis pathway of ubiquitin ligase in skeletal muscle may have been activated in the early stage of cerebral ischemia reperfusion injury in rats.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743.3
【參考文獻(xiàn)】
中國(guó)期刊全文數(shù)據(jù)庫(kù) 前10條
1 王靜;;偏癱早期良肢位擺放療效觀察[J];中國(guó)實(shí)用神經(jīng)疾病雜志;2008年09期
2 關(guān)瑩;邢艷麗;;針刺配合康復(fù)改善偏癱痙攣狀態(tài)30例[J];上海針灸雜志;2008年04期
3 王慧;羅勇;;Atrogin-1和MuRF-1在肌肉萎縮中的作用機(jī)制及影響因素[J];實(shí)用醫(yī)學(xué)雜志;2012年11期
4 Hafer-Macko C.E.;Ryan A.S.;牛亞利;;卒中后骨骼肌內(nèi)腫瘤壞死因子-α升高[J];世界核心醫(yī)學(xué)期刊文摘(神經(jīng)病學(xué)分冊(cè));2006年01期
5 劉巖,王芙蕊,李友蓮;早期康復(fù)訓(xùn)練對(duì)腦卒中病人運(yùn)動(dòng)功能的影響[J];中國(guó)臨床康復(fù);2002年01期
6 郄淑燕,岳壽偉;廢用性肌萎縮研究進(jìn)展[J];中國(guó)臨床康復(fù);2003年05期
7 郭民霞,俞世勛;腦卒中患者偏癱痙攣的康復(fù)[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2000年05期
8 高聰,蒲蜀湘,朱德儀;早期康復(fù)治療對(duì)腦卒中偏癱患者肢體功能及日常生活能力的影響[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2001年01期
9 劉鵬,黃東鋒,毛玉tb,江沁,陳少貞;早期康復(fù)對(duì)腦卒中患者功能恢復(fù)的影響[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2003年02期
10 白玉龍,胡永善;肉毒桿菌毒素治療中風(fēng)后肌痙攣的研究[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2004年01期
，本文編號(hào)：2222308
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