發(fā)布時間：2018-08-22 08:59

【摘要】：目的探討山楂葉總黃酮對慢性腦缺血大鼠腦組織軸突過度再生抑制因子(Nogo)A及其受體NgR1表達的影響。方法健康雄性SD大鼠,隨機分為假手術(shù)組、慢性腦缺血模型組、山楂葉總黃酮組和銀杏葉片組。采用雙側(cè)頸總動脈永久性雙重結(jié)扎法建立慢性腦缺血模型。HE染色法觀察神經(jīng)元密度;Western印跡和免疫組化法檢測缺血腦組織Nogo A和NgR1蛋白的表達。結(jié)果與假手術(shù)組海馬神經(jīng)細胞比較,模型組大鼠CA1區(qū)神經(jīng)細胞排列混亂,神經(jīng)元細胞間隙增大,部分細胞溶解消失,細胞核固縮、濃染,正常神經(jīng)元數(shù)量較假手術(shù)組減少(P0.01),腦組織內(nèi)Nogo A(P0.01)和NgR1(P0.05)蛋白表達升高;與模型組比較,山楂葉總黃酮組病變明顯減輕,正常的神經(jīng)細胞排列規(guī)則,結(jié)構(gòu)較完整,神經(jīng)元密度增加(P0.05),Nogo A(P0.01)和NgR1(P0.05)蛋白表達降低。結(jié)論山楂葉總黃酮具有下調(diào)Nogo A和NgR1蛋白表達,促進腦神經(jīng)元軸突再生,保護慢性腦缺血大鼠神經(jīng)的功能。
[Abstract]:Objective to investigate the effects of total flavonoids in Hawthorn leaves on the expression of (Nogo) A and its receptor NgR1 in brain tissue of rats with chronic cerebral ischemia. Methods healthy male Sprague-Dawley rats were randomly divided into sham-operation group, chronic cerebral ischemia model group, Hawthorn leaf flavonoid group and ginkgo leaf group. The model of chronic cerebral ischemia was established by permanent double ligation of bilateral common carotid artery. The expression of Nogo A and NgR1 in ischemic brain tissue was detected by Western blot and immunohistochemistry. Results compared with the sham operation group, the nerve cells in the CA1 region of the model group were disordered, the gap between the neurons increased, some cells dissolved and disappeared, and the nucleus became pyknosis and staining. Compared with the model group, the number of normal neurons decreased (P0.01), and the expression of Nogo A (P0.01 and NgR1 (P0.05) protein increased in brain tissue. The expression of Nogo A (P0.01) and NgR1 (P0.05) decreased. Conclusion Total flavonoids in Hawthorn leaves can down-regulate the expression of Nogo A and NgR1 proteins, promote axonal regeneration of brain neurons, and protect the nerves of rats with chronic cerebral ischemia.
【作者單位】： 承德醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院;承德醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)研究所河北省中醫(yī)藥抗癡呆重點研究室;
【基金】：河北省科技廳指令課題(No.15277789D) 河北省教育廳青年基金指令課題(No.QN2014103) 河北省高校省級重點學(xué)科項目(No.2013-2016) 河北省中醫(yī)藥抗癡呆重點研究室項目(No.2014-2017) 承德醫(yī)學(xué)院大學(xué)生科研重點課題(No.201505)
【分類號】：R743

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