【摘要】：目的:研究分析重組人粒細胞集落刺激因子對新生SD大鼠凋亡誘導(dǎo)因子表達的影響。方法:隨機選取7日齡SD大鼠36只,隨機分為假手術(shù)組(A組)、腦缺血再灌注組(B組)和藥物治療組(C組),每組各12只。采用Longa線栓法制作大鼠大腦動脈閉塞再灌注模型,C組大鼠在腦缺血2 h后及24 h時給予藥物灌注,其余2組給予大鼠注射等量的0.9%氯化鈉注射液。實驗結(jié)束后,采用Longa 5分制標準評分法在24 h時對大鼠的神經(jīng)功能進行評分,采用免疫組織化學(xué)法對大鼠的組織磷酸化c-jun氨基末端激酶、磷酸化細胞外信號調(diào)節(jié)蛋白激酶進行檢測,同時采用原位末端轉(zhuǎn)移酶標記對神經(jīng)細胞凋亡、TTC染色測腦梗死體積進行相關(guān)檢測。對凋亡相關(guān)基因的表達及凋亡水平進行評價。結(jié)果:A組僅有一小部分凋亡細胞,B、C 2組在灌注24 h后可以發(fā)現(xiàn)大量的凋亡細胞,細胞核均呈棕褐色,主要分布在缺血腦組織中周圍;C組腦梗死程度較B組減輕(P0.01)。結(jié)論:重組人粒細胞集落刺激因子可以起到保護新生SD大鼠神經(jīng)細胞的作用,避免發(fā)生缺血缺氧性腦損傷,這種糖蛋白與細胞凋亡相關(guān)基因的表達有關(guān)。
[Abstract]:Aim: to study the effect of recombinant human granulocyte colony stimulating factor (rhGCSF) on the expression of apoptosis-inducing factor in neonatal SD rats. Methods: Thirty-six 7-day-old SD rats were randomly divided into sham-operated group (group A), cerebral ischemia-reperfusion group (group B) and drug therapy group (group C) with 12 rats in each group. The cerebral artery occlusion reperfusion model of rats was established by Longa thread occlusion. Rats in group C were perfused with drugs at 2 hours and 24 hours after cerebral ischemia, and the other two groups were given the same amount of 0.9% sodium chloride injection. At the end of the experiment, the nerve function of the rats was evaluated by the standard Longa scoring method at 24 h, and the phosphorylated c-jun amino-terminal kinases in the tissues of the rats were assessed by immunohistochemical method. Extracellular signal regulated protein kinase (ESRK) was detected by phosphorylation and in situ terminal transferase (TTC) staining was used to detect the volume of cerebral infarction. The expression of apoptosis-related genes and the level of apoptosis were evaluated. Results A large number of apoptotic cells were found in group B _ (2) after 24 hours of perfusion, and the nuclei were brown, mainly distributed in ischemic brain tissue. The degree of cerebral infarction in group C was less than that in group B (P0.01). Conclusion: recombinant human granulocyte colony stimulating factor can protect newborn SD rat neurons from ischemic hypoxic brain injury. This glycoprotein is related to the expression of apoptosis-related genes.
【作者單位】： 上海交通大學(xué)醫(yī)學(xué)院附屬第九人民醫(yī)院兒科;
【基金】：上海市高校選拔優(yōu)秀青年教師專項基金(ZZjdyx12114)
【分類號】：R742

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