本文選題：促紅細(xì)胞生成素 + 皮層神經(jīng)元　； 參考：《吉林大學(xué)》2017年碩士論文

【摘要】：背景:促紅細(xì)胞生成素(EPO)對腦缺血缺氧性損傷具有顯著的神經(jīng)保護(hù)作用。它的作用機(jī)制有抗凋亡、抗氧化和抗炎作用以及刺激血管生成和神經(jīng)發(fā)生等。許多動(dòng)物實(shí)驗(yàn)或臨床研究的結(jié)果存在差異,可能與不同的實(shí)驗(yàn)方法包括缺氧持續(xù)時(shí)間、給藥時(shí)間、給藥劑量和頻率等有關(guān)。EPO對于腦缺血缺氧性損傷顯示巨大的神經(jīng)保護(hù)潛力,因此為更好地實(shí)現(xiàn)EPO從實(shí)驗(yàn)室到臨床的應(yīng)用,以上的每個(gè)因素都必須進(jìn)行研究及評估。目的:研究rhEPO對腦缺血缺氧性損傷的神經(jīng)保護(hù)作用及其有效劑量和作用時(shí)間。方法:分離新生的Wistar大鼠皮層神經(jīng)細(xì)胞進(jìn)行原代培養(yǎng),用SP法進(jìn)行神經(jīng)元純度鑒定。神經(jīng)元培養(yǎng)至7d-11d,隨機(jī)分為正常對照組、OGD損傷組和rhEPO處理組。對于rhEPO處理組神經(jīng)元,首先進(jìn)行OGD損傷,之后給予不同劑量的rhEPO(0.1、1、10、100U/ml)處理;另外,在不同的時(shí)間(OGD損傷后0h、24h、48h)給予損傷神經(jīng)元10U/ml rhEPO處理。觀察各組細(xì)胞形態(tài)學(xué)改變,MTT法檢測細(xì)胞存活率。結(jié)果:1、原代培養(yǎng)的皮層神經(jīng)元陽性率達(dá)90%以上,符合實(shí)驗(yàn)要求。2、與OGD損傷組比較,rhEPO(0.1、1、10U/ml)處理可增加OGD損傷的皮層神經(jīng)元的存活率(P0.05),且濃度為10U/ml時(shí)rhEPO的保護(hù)作用最強(qiáng)(P0.05)。皮層神經(jīng)元OGD損傷后立即加入rhEPO(10U/ml)保護(hù)作用最強(qiáng)(P0.05),損傷后24h、損傷后48h給藥沒有增加皮層神經(jīng)元存活率。結(jié)論:rhEPO對體外缺氧缺糖損傷的大鼠皮層神經(jīng)元具有保護(hù)作用,rhEPO有效濃度為0.1-10U/ml,且其濃度為10U/ml時(shí)保護(hù)作用最強(qiáng);rhEPO在神經(jīng)元損傷后立即給藥保護(hù)作用最強(qiáng)。
[Abstract]:Background: Erythropoietin (EPO) has a significant neuroprotective effect on cerebral ischemic hypoxic injury. Its mechanisms include anti-apoptosis, anti-oxidation and anti-inflammation, and stimulation of angiogenesis and neurogenesis. The results of many animal experiments or clinical studies may be related to different experimental methods, such as hypoxia duration, dosage and frequency of administration. EPO shows great neuroprotective potential in cerebral ischemic hypoxic injury. Therefore, in order to better realize the application of EPO from laboratory to clinical, each of the above factors must be studied and evaluated. Aim: to study the neuroprotective effect of rhEPO on cerebral ischemic hypoxic injury and its effective dose and time. Methods: the cortical neurons of newborn Wistar rats were isolated and cultured in primary culture, and the purity of neurons was identified by SP method. Neurons cultured for 7 d to 11 d were randomly divided into normal control group and rhEPO treated group. In rhEPO treated group, OGD injury was first performed, then rhEPO was given at different doses of rhEPO (0.1 ~ 10100U / ml), in addition, 10U / ml rhEPO was given at different time (0 h ~ 24h ~ 48h after OGD injury). Cell viability was measured by MTT assay. Results the positive rate of primary cultured cortical neurons was more than 90%. Compared with OGD group, rhEPO (0.1U / ml) could increase the survival rate of OGD injured cortical neurons (P0.05), and the protective effect of rhEPO was the strongest (P0.05) when the concentration was 10Uml. The protective effect of rhEPO (10U / ml) on cortical neurons was the strongest immediately after OGD injury (P0.05). 24 hours after injury and 48 hours after injury, the survival rate of cortical neurons was not increased by administration of rhEPO (10U / ml). Conclusion the effective concentration of rhEPO is 0.1-10U / ml, and the protective effect of rhEPO is strongest when the concentration is 10U / ml. The protective effect of rhEPO is strongest immediately after neuron injury.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 劉晶;王大鵬;姜巖;張廣萍;林再紅;杜姝;于繪麗;;依達(dá)拉奉聯(lián)合促紅細(xì)胞生成素治療進(jìn)展性缺血性腦卒中的臨床研究[J];齊齊哈爾醫(yī)學(xué)院學(xué)報(bào);2016年30期

2 陳穎東;宋清宇;;促紅細(xì)胞生成素在腦缺血再灌注損傷中的抑制細(xì)胞凋亡機(jī)制研究[J];山西醫(yī)藥雜志;2016年15期

3 王曉芳;王妮;呂曉紅;;促紅細(xì)胞生成素預(yù)處理在腦缺血性損傷中神經(jīng)保護(hù)作用的體外研究[J];中風(fēng)與神經(jīng)疾病雜志;2016年07期

4 袁奇超;蔣犁;朱麗華;虞大凡;;重組人促紅細(xì)胞生成素在腦白質(zhì)損傷新生鼠經(jīng)細(xì)胞外信號調(diào)節(jié)激酶信號通路對血管內(nèi)皮生長因子受體2的影響[J];中國醫(yī)學(xué)科學(xué)院學(xué)報(bào);2016年02期

5 黃林娟;;促紅細(xì)胞生成素治療老年腦梗死的臨床療效及安全性[J];中國老年學(xué)雜志;2015年24期

6 段淼;曹云濤;;促紅細(xì)胞生成素對新生鼠缺氧缺血性腦損傷后5-溴-2-脫氧尿嘧啶核苷表達(dá)的影響[J];中國臨床藥理學(xué)雜志;2015年23期

7 李錦程;武曉寧;趙海蘋;閔連秋;王榮亮;羅玉敏;;大腦中動(dòng)脈內(nèi)促紅細(xì)胞生成素或聯(lián)合tPA注射對大鼠腦缺血再灌注的作用及機(jī)制[J];首都醫(yī)科大學(xué)學(xué)報(bào);2015年05期

8 徐偉華;趙冬;戴晶;劉祺;許暉;黃嘯元;王業(yè)忠;;神經(jīng)元特異性烯醇化酶在神經(jīng)元氧糖剝奪損傷模型復(fù)氧后的表達(dá)變化[J];中國全科醫(yī)學(xué);2015年20期

9 陳麗;王志成;宋林敬;呂曉紅;;缺氧預(yù)適應(yīng)對體外培養(yǎng)神經(jīng)元的神經(jīng)保護(hù)作用存在一個(gè)時(shí)間窗[J];中風(fēng)與神經(jīng)疾病雜志;2015年04期

10 楊傳豪;趙冬;劉祺;姬云翔;李令建;許暉;戴晶;何學(xué)君;蔡云鵬;王業(yè)忠;;新生大鼠皮層神經(jīng)元體外無血清原代培養(yǎng)[J];重慶醫(yī)學(xué);2014年29期

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