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  本文選題：促紅細胞生成素 + 皮層神經(jīng)元　； 參考：《吉林大學(xué)》2017年碩士論文

【摘要】：背景:促紅細胞生成素(EPO)對腦缺血缺氧性損傷具有顯著的神經(jīng)保護作用。它的作用機制有抗凋亡、抗氧化和抗炎作用以及刺激血管生成和神經(jīng)發(fā)生等。許多動物實驗或臨床研究的結(jié)果存在差異,可能與不同的實驗方法包括缺氧持續(xù)時間、給藥時間、給藥劑量和頻率等有關(guān)。EPO對于腦缺血缺氧性損傷顯示巨大的神經(jīng)保護潛力,因此為更好地實現(xiàn)EPO從實驗室到臨床的應(yīng)用,以上的每個因素都必須進行研究及評估。目的:研究rhEPO對腦缺血缺氧性損傷的神經(jīng)保護作用及其有效劑量和作用時間。方法:分離新生的Wistar大鼠皮層神經(jīng)細胞進行原代培養(yǎng),用SP法進行神經(jīng)元純度鑒定。神經(jīng)元培養(yǎng)至7d-11d,隨機分為正常對照組、OGD損傷組和rhEPO處理組。對于rhEPO處理組神經(jīng)元,首先進行OGD損傷,之后給予不同劑量的rhEPO(0.1、1、10、100U/ml)處理;另外,在不同的時間(OGD損傷后0h、24h、48h)給予損傷神經(jīng)元10U/ml rhEPO處理。觀察各組細胞形態(tài)學(xué)改變,MTT法檢測細胞存活率。結(jié)果:1、原代培養(yǎng)的皮層神經(jīng)元陽性率達90%以上,符合實驗要求。2、與OGD損傷組比較,rhEPO(0.1、1、10U/ml)處理可增加OGD損傷的皮層神經(jīng)元的存活率(P0.05),且濃度為10U/ml時rhEPO的保護作用最強(P0.05)。皮層神經(jīng)元OGD損傷后立即加入rhEPO(10U/ml)保護作用最強(P0.05),損傷后24h、損傷后48h給藥沒有增加皮層神經(jīng)元存活率。結(jié)論:rhEPO對體外缺氧缺糖損傷的大鼠皮層神經(jīng)元具有保護作用,rhEPO有效濃度為0.1-10U/ml,且其濃度為10U/ml時保護作用最強;rhEPO在神經(jīng)元損傷后立即給藥保護作用最強。
[Abstract]:Background: Erythropoietin (EPO) has a significant neuroprotective effect on cerebral ischemic hypoxic injury. Its mechanisms include anti-apoptosis, anti-oxidation and anti-inflammation, and stimulation of angiogenesis and neurogenesis. The results of many animal experiments or clinical studies may be related to different experimental methods, such as hypoxia duration, dosage and frequency of administration. EPO shows great neuroprotective potential in cerebral ischemic hypoxic injury. Therefore, in order to better realize the application of EPO from laboratory to clinical, each of the above factors must be studied and evaluated. Aim: to study the neuroprotective effect of rhEPO on cerebral ischemic hypoxic injury and its effective dose and time. Methods: the cortical neurons of newborn Wistar rats were isolated and cultured in primary culture, and the purity of neurons was identified by SP method. Neurons cultured for 7 d to 11 d were randomly divided into normal control group and rhEPO treated group. In rhEPO treated group, OGD injury was first performed, then rhEPO was given at different doses of rhEPO (0.1 ~ 10100U / ml), in addition, 10U / ml rhEPO was given at different time (0 h ~ 24h ~ 48h after OGD injury). Cell viability was measured by MTT assay. Results the positive rate of primary cultured cortical neurons was more than 90%. Compared with OGD group, rhEPO (0.1U / ml) could increase the survival rate of OGD injured cortical neurons (P0.05), and the protective effect of rhEPO was the strongest (P0.05) when the concentration was 10Uml. The protective effect of rhEPO (10U / ml) on cortical neurons was the strongest immediately after OGD injury (P0.05). 24 hours after injury and 48 hours after injury, the survival rate of cortical neurons was not increased by administration of rhEPO (10U / ml). Conclusion the effective concentration of rhEPO is 0.1-10U / ml, and the protective effect of rhEPO is strongest when the concentration is 10U / ml. The protective effect of rhEPO is strongest immediately after neuron injury.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R743

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