本文選題：腦出血 + 發(fā)育期��； 參考：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：探討發(fā)育期大鼠基底節(jié)出血對(duì)大鼠行為發(fā)育及腦內(nèi)多巴胺轉(zhuǎn)運(yùn)體（DAT）表達(dá)的影響。 方法： 1.新生同窩SD大鼠（P10）隨機(jī)分為腦出血（CH）組、假手術(shù)（SH）組和正常對(duì)照（NC）組，每組各16只。 2.利用立體定位儀向SD大鼠腦尾狀核注入自體血，建立腦出血模型，SH組只刺入而不注血，NC組不做任何處理。 3.分別于術(shù)后第1天（即P11）、術(shù)后第3天（即P13）、術(shù)后第7天（即P17）、術(shù)后第14d天即P24）采用Berderson評(píng)分法進(jìn)行大鼠神經(jīng)功能評(píng)分。 4.術(shù)后第14天（即P24)通過開場(chǎng)實(shí)驗(yàn)及Lat迷宮進(jìn)行大鼠行為學(xué)檢測(cè)，連續(xù)測(cè)試3天。 5.術(shù)后第17天（即P27)采用H.E染色觀察大鼠腦出血后病理變化，免疫組織化學(xué)染色測(cè)定DAT的表達(dá)。 結(jié)果： 1.CH組大鼠開場(chǎng)實(shí)驗(yàn)中的穿越格子數(shù)、直立次數(shù)均較SH組及NC組大鼠明顯增多，差異有統(tǒng)計(jì)學(xué)意義(P 0．05)； CH組大鼠在Lat迷宮內(nèi)穿越角落數(shù)、直立次數(shù)、理毛次數(shù)均較SH組及NC組大鼠顯著增加,差異有統(tǒng)計(jì)學(xué)意義(P 0．05)。 2. H.E染色顯示CH組血腫處可見一小空腔形成，可見少許淋巴細(xì)胞浸潤(rùn)及少許膠質(zhì)瘢痕形成；SH組未見明顯血腫，亦無(wú)空腔形成；NC組無(wú)炎性細(xì)胞浸潤(rùn)，細(xì)胞分布正常。 3. CH組大鼠腦內(nèi)DAT免疫反應(yīng)陽(yáng)性產(chǎn)物較NC組及SH組顯著增多，差異具有統(tǒng)計(jì)學(xué)意義(P 0．05)；而SH組與NC組比較差異無(wú)統(tǒng)計(jì)學(xué)意義(PO.O5)。 4.基底節(jié)區(qū)DAT蛋白與大鼠開場(chǎng)實(shí)驗(yàn)中穿越格子數(shù)、直立次數(shù)、理毛次數(shù)及Lat迷宮中穿越角落數(shù)、直立次數(shù)、理毛次數(shù)成正相關(guān)，具有統(tǒng)計(jì)學(xué)意義（P 0.05）。 結(jié)論： 1.發(fā)育期大鼠基底節(jié)局灶性出血，可修復(fù)性、代償性強(qiáng)，，多不遺留嚴(yán)重神經(jīng)運(yùn)動(dòng)功能障礙。 2.腦基底節(jié)出血所致的神經(jīng)損傷可導(dǎo)致發(fā)育期大鼠出現(xiàn)自主活動(dòng)增多、非選擇性注意水平降低等多動(dòng)、注意缺陷等遠(yuǎn)期行為異常。 3.腦內(nèi)多巴胺轉(zhuǎn)運(yùn)體（DAT）表達(dá)水平的增高可能與發(fā)育期大鼠腦基底節(jié)損傷后多動(dòng)、注意缺陷等發(fā)育行為異常有關(guān)。
[Abstract]:Aim: to investigate the effects of basal ganglia hemorrhage on behavioral development and dopamine transporter (DAT) expression in rat brain. Methods: 1. Neonatal Sprague-Dawley rats (P10) were randomly divided into three groups: intracerebral hemorrhage (Ch) group, sham operation (SH) group and normal control (NC) group, with 16 rats in each group. Autologous blood was injected into the caudate nucleus of the brain of SD rats by stereotactic locator. On the 1st day (P11), the 3rd day (P13), the 7th day (P17) and the 14th day after operation (P24), the neurological function score of rats was evaluated by Berderson's scoring method. On the 14th day after operation (P24), the behavior of rats was measured by open field experiment and Lat maze. The rats were tested continuously for 3 days. On the 17th day after operation (P27), the expression of DAT was determined by immunohistochemical staining, and the pathological changes of rats with intracerebral hemorrhage (ICH) were observed by H. E staining. Results: 1. Compared with SH group and NC group, the number of grid crossing and the number of upright in Ch group were significantly higher than those in SH group and NC group (P 0.05), the rats in Ch group passed through corner number in Lat labyrinth, and the number of upright rats in Ch group was significantly higher than that in SH group and NC group (P < 0.05). Compared with SH group and NC group, the times of shaving were significantly higher than that of SH group and NC group, the difference was statistically significant (P 0.05). E staining showed that there was a small cavity formation in hematoma, a little lymphocyte infiltration and a little glial scar formation in Ch group. No hematoma was found in SH group, and no inflammatory cell infiltration was found in NC group, and the cell distribution was normal in NC group. The expression of DAT immunoreactive products in brain of Ch group was significantly higher than that of NC group and SH group (P 0.05), but there was no significant difference between SH group and NC group (PO. O 5). 4. DAT protein in basal ganglia area was positively correlated with the number of traverses, the number of upright positions, the number of haircuts and the number of crossing corners in Lat labyrinth in the opening experiment of rats (P 0.05). Conclusion: 1. The focal hemorrhage of basal ganglia in developing rats is repairable and compensatory, and it does not leave behind serious neuromotor dysfunction. 2. The nerve injury caused by basal ganglia hemorrhage may lead to the increase of spontaneous activity, the decrease of non-selective attention level, the abnormal long-term behavior of attention deficit and so on. 3. The increased expression of dopamine transporter (DAT) in brain may be related to abnormal developmental behavior such as hyperactivity and attention deficit after basal ganglion injury in developing rats.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R743.34

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