發(fā)布時(shí)間：2018-06-20 05:21

  本文選題：內(nèi)皮祖細(xì)胞 + 胰島素樣生長(zhǎng)因子��； 參考：《中國(guó)動(dòng)脈硬化雜志》2017年02期

【摘要】：目的探討頸動(dòng)脈狹窄與內(nèi)皮祖細(xì)胞(EPC)的相互關(guān)系及胰島素樣生長(zhǎng)因子1(IGF-1)對(duì)EPC的調(diào)節(jié)作用及其主要機(jī)制。方法入選腦梗死伴頸動(dòng)脈狹窄患者35例為頸動(dòng)脈狹窄組,同時(shí)入選健康對(duì)照者11名為對(duì)照組。根據(jù)腦血管造影結(jié)果,頸動(dòng)脈狹窄組又分為輕度狹窄組、中度狹窄組、重度狹窄組。測(cè)定研究對(duì)象的血清IGF-1濃度。采用密度梯度離心法分離出單個(gè)核細(xì)胞,用內(nèi)皮細(xì)胞生長(zhǎng)培養(yǎng)基(EGM2)培養(yǎng),雙染法鑒定EPC。實(shí)驗(yàn)分為4組:未處理組、IGF-1組、IGF-1+氮-硝基-左旋-精氨酸甲基酯(L-NAME)組、L-NAME組,使用EGM2培養(yǎng)細(xì)胞2~3周,分別測(cè)定各組EPC的增殖、黏附功能。結(jié)果頸動(dòng)脈狹窄程度越重,形成EPC集落數(shù)量越少(P0.05),血清IGF-1濃度也越低,并且隨著狹窄程度增加,EPC增殖、黏附能力降低。功能實(shí)驗(yàn)顯示IGF-1組EPC功能明顯高于未處理組(P0.01),IGF-1+L-NAME組未見(jiàn)明顯差異,L-NAME組低于未處理組。IGF-1組細(xì)胞內(nèi)皮型一氧化氮合酶(eNOS)明顯高于未處理組,IGF-1+L-NAME組未見(jiàn)明顯差異,L-NAME組低于未處理組。結(jié)論 EPC對(duì)頸動(dòng)脈狹窄可能具有保護(hù)作用,IGF-1可能通過(guò)影響eNOS的合成增強(qiáng)EPC的功能。
[Abstract]:Objective to investigate the relationship between carotid artery stenosis and endothelial progenitor cell (EPC), the regulatory effect of insulin-like growth factor 1 (IGF-1) on EPC and its main mechanism. Methods 35 patients with cerebral infarction accompanied with carotid artery stenosis were selected as carotid artery stenosis group and 11 healthy controls as control group. According to the results of cerebral angiography, carotid stenosis group was divided into mild stenosis group, moderate stenosis group and severe stenosis group. Serum IGF-1 concentration was determined. Mononuclear cells were isolated by density gradient centrifugation and cultured on endothelial cell growth medium (EGM2). EPCs were identified by double staining. The experiment was divided into 4 groups: untreated group IGF-1 nitrogen-nitro-L-arginine methyl ester L-NAME-group, L-NAME group, cultured cells were cultured with EGM2 for 2 weeks. The proliferation and adhesion function of EPC in each group were measured. Results the more severe the carotid artery stenosis, the less EPC colony was formed and the lower the serum IGF-1 concentration was, and with the increase of stenosis degree, the proliferation and adhesion ability of EPC were decreased. The functional test showed that the EPC function of IGF-1 group was significantly higher than that of untreated group (P0.01-IGF-1 L-NAME group). The EPC function of IGF-1 group was significantly higher than that of untreated group (P 0.01-IGF-1 L-NAME group), and that of L-NAME group was lower than that of untreated group (P < 0.05), and that of L-NAME group was lower than that of untreated group. Conclusion EPC may have protective effect on carotid stenosis. IGF-1 may enhance the function of EPC by affecting the synthesis of Enos.
【作者單位】： 十堰市太和醫(yī)院湖北醫(yī)藥學(xué)院附屬醫(yī)院神經(jīng)內(nèi)科;
【基金】：湖北省自然科學(xué)基金項(xiàng)目(2011CDB131)
【分類(lèi)號(hào)】：R743.33

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 單振宇,周定標(biāo),王瑞恒,許成吉,徐龍慶,曹玉福,白成濤;頸動(dòng)脈狹窄手術(shù)治療(附4例報(bào)告)[J];中國(guó)煤炭工業(yè)醫(yī)學(xué)雜志;2002年09期

2 胡立;頸動(dòng)脈狹窄的術(shù)前診斷[J];天津醫(yī)藥;2004年02期

3 張劍權(quán),吳海紅,黃桂林;頸動(dòng)脈狹窄的診斷與治療[J];農(nóng)墾醫(yī)學(xué);2004年03期

4 趙志青,景在平,陸清聲,包俊敏,馮翔,趙s，

本文編號(hào)：2043124