本文選題：異黑成熟顆粒 + 帕金森病　； 參考：《新疆醫(yī)科大學》2014年碩士論文

【摘要】：目的：探討維藥異黑成熟顆粒對帕金森病(Parkinson's disease,PD)模型大鼠行為學及氧化應(yīng)激反應(yīng)的影響。方法：采用6-羥基多巴胺(6-OHDA)立體定位單側(cè)雙位點毀損法制備PD大鼠模型。設(shè)立正�？瞻讓φ战M,并將造模成功的PD模型大鼠隨機分成模型空白對照組、美多巴用藥組(美多巴125mg/kg)、維藥異黑成熟顆粒低、中、高劑量組(分別為1g/kg、3g/kg、6g/kg),每組10只大鼠,觀察給藥前后大鼠的行為學變化,給藥40天后用免疫組化法檢測右側(cè)中腦黑質(zhì)部酪氨酸羥化酶(TH)細胞表達數(shù)量變化及中腦黑質(zhì)部超氧化物歧化酶(SOD)、谷胱甘肽過氧化物酶(GSH-PX)活性及脂質(zhì)過氧化氫丙二醛(MDA)含量的變化。結(jié)果：行為學方面,維藥低劑量組旋轉(zhuǎn)圈數(shù)(r/min)為(12.50±1.50),與模型空白對照組比較差異無統(tǒng)計學意義(P0.05),美多巴用藥組、維藥中、高劑量組旋轉(zhuǎn)圈數(shù)(r/min)為(6.00±1.07)、(10.38±1.69)、(7.00±1.31),與模型空白對照組比較差異有統(tǒng)計學意義(P0.01)。TH細胞表達方面,維藥低劑量組TH數(shù)(個)為(9.13±2.23),與模型空白對照組比較差異無統(tǒng)計學意義(P0.05),美多巴用藥組、維藥中、高劑量組TH數(shù)(個)分別為(50.38±2.33)、(25.36±2.56)、(48.13±4.00),與模型空白對照組比較差異有統(tǒng)計學意義(P0.01)。氧化應(yīng)激方面,維藥低劑量組SOD含量為(111.74±6.22)U/mg、GSH-PX含量為(177.95±4.59) U/mg,MDA含量為(7.02±0.40)nmol/ml,與模型空白對照組比較差異均無統(tǒng)計學意義(P0.05),美多巴用藥組、維藥中、高劑量組SOD含量為(146.82±6.19).(123.27±5.74).(140.48±11.05)U/mg,GSH-PX含量為(222.92±4.83).(185.46±8.20).(219.03±2.66)U/mg,MDA含量為(4.89±0.24).(6.12±0.29).(5.21±0.38) nmol/ml,與模型空白對照組比較差有統(tǒng)計學意義(P0.01)。維藥高劑量組在行為學、TH細胞表達、氧化應(yīng)激方面,與美多巴用藥組比較差異無統(tǒng)計學意義(P0.05),維藥中劑量組在行為學、TH細胞表達、氧化應(yīng)激方面,與美多巴用藥組比較差異有統(tǒng)計學意義(P0.05),與維藥高劑量組比較差異有統(tǒng)計學意義(P0.01)。結(jié)論：維藥異黑成熟顆粒高、中劑量可改善PD大鼠的行為學表現(xiàn),可能具有提高機體抗氧化的能力,其中維藥高劑量組作用效果與美多巴用藥組相似,優(yōu)于維藥中、低劑量組。
[Abstract]:Aim: to investigate the effect of Yiyimairiao granule on the behavior and oxidative stress of Parkinsonian disease (PDD) model rats with Parkinson's disease (PD). Methods: PD rat model was established by 6-hydroxydopamine 6-OHDAs stereotactic method. Normal blank control group was set up, and the successful PD model rats were randomly divided into model blank control group, medopa drug group (medopa 125 mg / kg 路kg ~ (-1), medium and high dose group (1 g / kg ~ (-3) g / kg ~ (-1) mg / kg ~ (-1) mg 路kg ~ (-1), 10 rats in each group, and 10 rats in each group were treated with medopa (125 mg / kg 路kg ~ (-1) 路kg ~ (-1). The behavioral changes of rats before and after administration were observed. The expression of tyrosine hydroxylase (THH) and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and lipids peroxide in the substantia nigra were detected by immunohistochemical method 40 days after administration. Changes of MDAs in aldehydes. Results: in the behavioral aspect, the number of rotation circles in the low dose group was 12.50 鹵1.50g / min, which was not significantly different from that in the model control group (P 0.05). The number of rotation circles in the high dose group was 6.00 鹵1.07U / min (10.38 鹵1.69) and 7.00 鹵1.31g respectively. There was a significant difference in the expression of th between the high dose group and the model control group. The th number in the low dose group was 9.13 鹵2.230.There was no significant difference between the low dose group and the model control group (P 0.05), and the expression of th in the Madopa group was higher than that in the model control group, and there was no significant difference in the number of th between the low dose group and the model control group (P 0.05), while in the Madopa group, the number of th was 9.13 鹵2.23. The th number in the high dose group was 50.38 鹵2.33, 25.36 鹵2.56, 48.13 鹵4.00, respectively, which was significantly higher than that in the model control group (P 0.01). In terms of oxidative stress, the content of SOD in low dose group was 111.74 鹵6.22 U / mg / g GSH-PX (177.95 鹵4.59). The content of MDA was 7.02 鹵0.40 nmol / ml. There was no significant difference compared with the model control group (P 0.05), and the content of GSH-PX in Madopa group was higher than that in control group. In the high dose group, the content of SOD was 146.82 鹵6.19U, 123.27 鹵5.74, 140.48 鹵11.05 渭 g / g, GSH-PX was 222.92 鹵4.83N, 185.46 鹵8.20 鹵8.20 鹵2.66 U / mg MDA, the content of MDA was 4.89 鹵0.24.6.12 鹵0.29 鹵0.29 鹵0.21 鹵0.38 nmol / ml, which was significantly different from that of the model control group (P < 0.01). There was no significant difference in the expression of th cells and oxidative stress between the high dose group and Madopa group, but there was no significant difference in the expression of th cells and oxidative stress between the medium dose group and the Madopa group. There was a significant difference between Madopa group and Madopa group (P 0.05), and the difference was statistically significant compared with the high dose group (P 0.01). Conclusion: the behavior of PD rats can be improved by the medium dose of Weiyangyimiriao granules, which may improve the ability of anti-oxidation. The effect of high dose group is similar to that of medopa group, which is better than that of medium and low dose group.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R742.5

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