本文選題：Fascin-1 + 顳葉癲癇　； 參考：《重慶醫(yī)科大學》2014年碩士論文

【摘要】：目的：肌成束蛋白Fascin-1是一種廣泛表達于中樞神經(jīng)系統(tǒng)的細胞骨架蛋白，，在細胞形態(tài)及功能發(fā)展中起著重要的作用。許多研究發(fā)現(xiàn)Fascin-1與突觸重塑有著密切的關系，且Fascin-1的異常調節(jié)與許多中樞神經(jīng)系統(tǒng)（CNS）疾病有關。突觸重塑是顳葉癲癇（TLE）重要的病理基礎，目前仍不清楚Fascin-1是否也參與到顳葉癲癇的病理過程中呢。因此，本研究旨在研究在顳葉癲癇患者及匹魯卡品誘導的大鼠模型中Fascin-1蛋白的表達情況，并探討Fascin-1與顳葉癲癇的關系及意義。 方法：從本課題組已建立的顳葉癲癇患者腦組織庫中隨機選取20例顳葉癲癇病灶腦組織標本做為病例組進行研究，并收集10例無癲癇病史的腦外傷患者經(jīng)減壓手術后獲得的的組織結構正常的顳葉皮質做為對照組；40只雄性SD大鼠被分為對照組（5只）、實驗組（35只），實驗組大鼠腹腔注射匹羅卡品建立顳葉癲癇模型，在模型點燃后的不同時間點（6h、24h、72h、1W、2W、1M、2M月）斷頭取腦；分別運用免疫組化、Western blot檢測癲癇患者及大鼠模型腦組織中Fascin-1蛋白的動態(tài)變化情況；免疫熒光雙標被用來對Fascin-1的表達進行定位。 結果：Fascin-1主要在顳葉皮質及海馬的神經(jīng)元細胞胞漿及胞膜中表達，不表達于膠質細胞；且Fascin-1在顳葉癲癇患者中的表達水平明顯低于正常對照組（P＜0.05）。同樣，在癲癇模型大鼠顳葉皮質及海馬中，F(xiàn)ascin-1的表達水平在各個時間點都低于對照組（P＜0.05，皮質6h時間點除外）。皮質表達最低的時間點是在2M，海馬表達最低的時間點是在2W。 結論：Fascin-1蛋白水平在顳葉癲癇患者及匹羅卡品大鼠模型中都被下調，提示Fascin-1蛋白的降低可能與顳葉癲癇的病理過程有著密切關系。
[Abstract]:Aim: myobundle protein (Fascin-1) is a cytoskeletal protein widely expressed in the central nervous system (CNS), which plays an important role in the development of cell morphology and function. Many studies have found that Fascin-1 is closely related to synaptic remodeling, and the abnormal regulation of Fascin-1 is related to many central nervous system (CNS) diseases. Synaptic remodeling is an important pathological basis of temporal lobe epilepsy. It is not clear whether Fascin-1 is also involved in the pathogenesis of temporal lobe epilepsy. Therefore, the purpose of this study was to investigate the expression of Fascin-1 protein in patients with temporal lobe epilepsy and pilocarpine induced rat model, and to explore the relationship between Fascin-1 and temporal lobe epilepsy. Methods: twenty brain tissue specimens of temporal lobe epilepsy were randomly selected from the brain tissue bank of patients with temporal lobe epilepsy. Ten cases of traumatic brain injury without history of epilepsy were collected as control group. 40 male Sprague-Dawley rats were divided into control group (n = 5), experimental group (n = 35) and experimental group (n = 35). Temporal lobe epilepsy model was established by intravenously injection of pilocarpine. At different time points after model kindling, the brain was cut off at different time points (6h / 24h / 72h / 72h / 72W / W / M), and the dynamic changes of Fascin-1 protein in brain tissue of epileptic patients and rats were detected by immunohistochemical Western blot, and the expression of Fascin-1 was detected by immunofluorescence double labeling. Results the expression of Fascin-1 was mainly in the cytoplasm and membrane of neurons in temporal cortex and hippocampus, but not in glial cells, and the expression level of Fascin-1 in patients with temporal lobe epilepsy was significantly lower than that in normal controls (P < 0.05). Similarly, the expression level of Fascin-1 in temporal cortex and hippocampus of epileptic model rats was lower than that of control group at all time points (P < 0.05), except for cortex at 6 h. The lowest expression of cortex was at 2M, and that of hippocampus was at 2W. Conclusion the level of Fascin-1 protein is down-regulated in patients with temporal lobe epilepsy and pilocarpine rat model, suggesting that the decrease of Fascin-1 protein may be closely related to the pathological process of temporal lobe epilepsy.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R742.1

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1 詹傲;石全紅;張超;譚贏;劉瀏;謝延風;但煒;王佳;;Fascin-1在顳葉癲癇大鼠中表達的變化[J];第三軍醫(yī)大學學報;2014年15期

本文編號：1982998