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  本文選題：神經(jīng)膠質(zhì)瘤 + 瘤周水腫��； 參考：《中南大學》2014年碩士論文

【摘要】：目的：研究Wistar大鼠腦膠質(zhì)瘤瘤周水腫組織的磁共振灌注成像(MR-PWI)的特征,并與免疫組化水通道蛋白-1(AQPl)對照,探討膠質(zhì)瘤瘤周水腫組織AQP1的表達與局部腦血容量(rCBV)的相關性。 方法：通過立體定向技術將C6細胞株種植于35只Wistar大鼠右側(cè)大腦尾狀核區(qū),種植約3-4周后所有存活大鼠行常規(guī)MRI、PWI及T1WI增強檢查。借助Function Tool軟件對PWI數(shù)據(jù)后處理,選取瘤周水腫組織及相應對側(cè)正常腦組織等大的3個感興趣區(qū),獲得相應部位rCBV值。MRI掃描結(jié)束后立即處死大鼠,取全腦固定并做瘤周水腫區(qū)腦組織的連續(xù)切片,行相應部位的HE染色及AQP1免疫組化染色。利用Pearson相關分析法確定rCBV值與AQP1陽性表達IOD值之間的關系。 結(jié)果：接種C6細胞株3-4周后,大鼠膠質(zhì)瘤瘤周水腫組織3個等大的感興趣區(qū)rCBV平均值為(244.34±45.96),與對側(cè)正常腦組織rCBV平均值(205.18±30.46)相比明顯升高,差異具有統(tǒng)計學意義(P0.05)。瘤周水腫組織AQP1表達的IOD平均值為(76595.35±1116.98),瘤周水腫組織的rCBV值與AQP1陽性表達IOD值間均存在正相關(r=0.51,P0.05)。 結(jié)論：Wistar大鼠C6腦膠質(zhì)瘤瘤周水腫組織的rCBV值較對側(cè)腦組織升高,且可以反映AQP1的表達水平。
[Abstract]:Objective: to study the characteristics of Mr perfusion imaging (MR-PWI) in the peritumoral edema of gliomas in Wistar rats, and to investigate the correlation between the expression of AQP1 and regional cerebral blood volume (CBV) in glioma peri-tumorous edema tissues, as compared with immunohistochemical aquaporin-1 aquaporin (AQP). Methods: C6 cell lines were implanted in the right caudate nucleus of the brain of 35 Wistar rats by stereotactic technique. After implantation for about 3-4 weeks, all the surviving rats were examined with conventional MRI PWI and T1WI enhancement. Using Function Tool software to post-process the PWI data, three large regions of interest, such as the edema tissue around the tumor and the corresponding contralateral normal brain tissue, were selected, and the corresponding rCBV value was obtained. The rats were killed immediately after the scanning. The whole brain was fixed and serial sections of the brain tissue in the peritumoral edema area were made. The HE staining and AQP1 immunohistochemical staining were performed in the corresponding areas. Pearson correlation analysis was used to determine the relationship between rCBV value and IOD value of AQP1 positive expression. Results: after 3 to 4 weeks of C6 cell line inoculation, the average rCBV of 3 isometric regions of interest was 244.34 鹵45.96, which was significantly higher than that of the contralateral normal brain tissue (205.18 鹵30.46), and the difference was statistically significant (P 0.05). The average IOD expression of AQP1 in peritumoral edema tissue was 76595.35 鹵1116.98, and there was a positive correlation between rCBV value and AQP1 positive expression IOD value in peritumoral edema tissue. Conclusion the rCBV value of brain edema tissue around C6 glioma is higher than that of contralateral brain tissue, and can reflect the expression level of AQP1.
【學位授予單位】：中南大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R739.4

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本文編號：1935114