本文選題：超聲 + 剪切波彈性成像��； 參考：《中國(guó)人民解放軍醫(yī)學(xué)院》2016年博士論文

【摘要】：目的：剪切波彈性成像技術(shù)是目前彈性成像領(lǐng)域的前沿技術(shù),其臨床應(yīng)用范圍正在迅速的不斷擴(kuò)大。目前剪切波彈性成像技術(shù)已開始應(yīng)用于新生兒顱腦疾病的診斷,有研究證實(shí)該技術(shù)具有一定臨床診斷價(jià)值。但生長(zhǎng)發(fā)育過程中的中樞神經(jīng)系統(tǒng)對(duì)外界刺激存在敏感性,若這一過程受到干擾,將會(huì)給生物體帶來嚴(yán)重的后果。因而該技術(shù)應(yīng)用于新生兒顱腦檢查是否在安全范圍仍有待考證,國(guó)內(nèi)外尚無研究對(duì)此進(jìn)行報(bào)道。本課題以新生小鼠為實(shí)驗(yàn)對(duì)象,首次研究剪切波彈性成像顱腦檢查技術(shù)對(duì)新生哺乳動(dòng)物腦組織及腦功能的影響,研究?jī)?nèi)容主要包括以下兩個(gè)方面：1.采用剪切波彈性成像儀對(duì)新生C57乳鼠進(jìn)行不同時(shí)長(zhǎng)的照射,并在照射后24小時(shí)取腦,觀察腦組織形態(tài)學(xué)變化及信號(hào)通路相關(guān)蛋白在腦組織中的表達(dá)；2.將經(jīng)過不同時(shí)長(zhǎng)照射的C57乳鼠飼養(yǎng)至3個(gè)月,使用Morris水迷宮檢測(cè)小鼠的認(rèn)知功能,并在水迷宮實(shí)驗(yàn)后斷頭取腦,觀察腦組織形態(tài)學(xué)變化及相關(guān)蛋白的表達(dá)情況。方法：第一部分：將225只出生24小時(shí)的新生乳鼠隨機(jī)分為五組：對(duì)照組,5分鐘組,10分鐘組,20分鐘組和30分鐘組,并根據(jù)相應(yīng)分組對(duì)乳鼠進(jìn)行不同時(shí)長(zhǎng)的剪切波彈性成像儀的照射。在接受照射后24小時(shí),每組取10只乳鼠斷頭取腦,觀察腦組織形態(tài)學(xué)變化和信號(hào)通路相關(guān)蛋白的表達(dá)情況。第二部分：將剩余小鼠飼養(yǎng)至3個(gè)月,每組隨機(jī)挑選15只雄性小鼠,利用Morris水迷宮對(duì)小鼠的空間學(xué)習(xí)記憶能力進(jìn)行檢測(cè),觀察并統(tǒng)計(jì)逃避潛伏期、穿臺(tái)次數(shù)及目標(biāo)象限停留時(shí)間等指標(biāo),并在水迷宮實(shí)驗(yàn)24小時(shí)內(nèi)將小鼠斷頭取腦,觀察腦組織形態(tài)學(xué)變化及信號(hào)通路相關(guān)蛋白在腦組織中的表達(dá)情況。結(jié)果：第一部分：根據(jù)HE染色和NissI染色的結(jié)果,對(duì)照組,5分鐘組,10分鐘組,20分鐘組和30分鐘組的乳鼠腦組織均未發(fā)現(xiàn)明顯病理變化。Western-blot檢測(cè)的結(jié)果顯示,10分鐘,20分鐘和30分鐘組乳鼠腦組織中的p-PKCa蛋白水平減低,30分鐘組乳鼠腦組織中的p-mTOR和p-AKT蛋白水平減低。第二部分：根據(jù)Morris水迷宮的實(shí)驗(yàn)結(jié)果,對(duì)照組,5分鐘組,10分鐘組,20分鐘組和30分鐘組的小鼠之間逃避潛伏期、穿臺(tái)次數(shù)及目標(biāo)象限停留時(shí)間等指標(biāo)的差異均無統(tǒng)計(jì)學(xué)意義。將小鼠斷頭取腦后,根據(jù)HE染色和Nissl染色的結(jié)果,各組小鼠腦組織均未發(fā)現(xiàn)明顯病理改變；Western-blot檢測(cè)的結(jié)果顯示,各組小鼠之間腦組織相關(guān)通路蛋白的表達(dá)水平無明顯差異。結(jié)論：利用剪切波彈性成像儀對(duì)新生乳鼠持續(xù)照射30分鐘,雖不會(huì)引起乳鼠腦組織形態(tài)學(xué)的改變,但當(dāng)照射時(shí)間持續(xù)10分鐘時(shí),腦組織中p-PKCa蛋白水平表達(dá)減低；持續(xù)30分鐘時(shí),腦組織中P13K/AKT/mTOR信號(hào)通路會(huì)受到影響。這種改變隨著小鼠的長(zhǎng)大會(huì)逐漸得到修復(fù),且其空間學(xué)習(xí)記憶能力與對(duì)照組小鼠相比無明顯差異,但仍提示生長(zhǎng)發(fā)育過程中的中樞神經(jīng)系統(tǒng)對(duì)聲輻射力脈沖刺激存在敏感性,在利用剪切波彈性成像儀對(duì)新生兒顱腦進(jìn)行檢測(cè)時(shí),應(yīng)避免長(zhǎng)時(shí)間照射。
[Abstract]:Objective: shear wave elastic imaging is the frontier technology in the field of elastic imaging and its clinical application is expanding rapidly. At present, shear wave elastography has been applied to the diagnosis of neonatal craniocerebral diseases. The nervous system is sensitive to external stimuli. If this process is disturbed, it will bring serious consequences to the organism. Therefore, the application of this technique to the safety of newborn brain examination still remains to be examined, and there is no study on it at home and abroad. The effects of brain examination on brain tissue and brain function of newborn mammals were mainly included in the following two aspects: 1. using shear wave elastic imaging apparatus to irradiate newborn C57 rats at the same time, and take the brain for 24 hours after irradiation, and observe the changes of brain histomorphology and signal pathway related proteins in the brain group. The expression in the fabric; 2. the C57 milk rats, which were irradiated with different length of time, were reared for 3 months, and the cognitive function of the mice was detected by the Morris water maze. After the water maze experiment, the brain was cut off, and the changes of the brain histomorphology and the expression of related proteins were observed. Method: the first part: 225 newborn rats were randomly divided into 225 newborn infants, which were born and 24 hours randomly. The five groups: the control group, the 5 minute group, the 10 minute group, the 20 minute group and the 30 minute group, and according to the corresponding group, they were irradiated by the different long shear wave elastic imaging apparatus. 24 hours after receiving the irradiation, 10 milk rats in each group were taken head to take the brain, and the expression of the brain tissue morphologic changes and signal pathway related proteins was observed. Second part. After feeding the remaining mice for 3 months, 15 male mice were randomly selected in each group. The Morris water maze was used to detect the spatial learning and memory ability of the mice. The time of escape latency, the number of times and the time of the target quadrant were counted, and the brain was taken from the head of the mice within 24 hours of the water maze test, and the morphology of the brain was observed. The expression of change and signal pathway related protein in brain tissue. Results: first part: according to the results of HE staining and NissI staining, the control group, 5 minute group, 10 minute group, 20 minute group and 30 minute group of milk rat brain tissues have no obvious pathological changes of.Western-blot detection results, 10 minutes, 20 minutes and 30 minutes group The level of p-PKCa protein in the brain tissue of the rats decreased, and the level of p-mTOR and p-AKT protein in the brain tissue of the rats was reduced in 30 minutes. Second parts: according to the experimental results of the Morris water maze, the control group, the 5 minute group, the 10 minute group, the 20 minute group and the 30 minute group of mice escape the latent period, the times of wearing the platform and the residence time of the target quadrant and so on. There was no significant difference in the standard difference. After taking the head of the mouse, the brain tissue of each group was not found obvious pathological changes according to the results of HE staining and Nissl staining. The results of Western-blot detection showed that there was no significant difference in the expression level of brain related pathway proteins between each group. Conclusion: shear wave elastic imaging is used. The expression of p-PKCa protein in brain tissue decreased when irradiation time lasted for 10 minutes, and the expression of p-PKCa protein in brain tissue was affected by the duration of irradiation for 10 minutes. This change was gradually repaired as the mice grew up. There is no significant difference in spatial learning and memory ability between the control group and the control group, but it still suggests that the central nervous system in the growth and development process is sensitive to acoustic radiation impulse stimulation. The long time exposure should be avoided in the detection of neonatal craniocerebral using shear wave elastic imaging instrument.
【學(xué)位授予單位】：中國(guó)人民解放軍醫(yī)學(xué)院
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2016
【分類號(hào)】：R742

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 方玲;何光彬;孟欣;張苗苗;秦海英;王莉;周曉東;;實(shí)時(shí)組織彈性成像在評(píng)價(jià)肝臟腫瘤中的初步應(yīng)用[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2010年10期

2 何興無;;射頻信號(hào)上的快速?gòu)椥猿上裎灰乒烙?jì)方法[J];重慶醫(yī)學(xué);2011年27期

3 王彬;凌濤;沈勇;鄭海榮;鄒建中;李發(fā)琪;;準(zhǔn)靜態(tài)彈性成像技術(shù)檢測(cè)聚焦超聲致離體組織損傷[J];中國(guó)醫(yī)學(xué)影像技術(shù);2011年11期

4 王一嬌;唐少珊;;彈性成像在超聲診斷中的應(yīng)用和前景[J];山東醫(yī)藥;2011年44期

5 A.Goddi;A.Sacchi;G.Magistretti;J.Almolla;M.Salvadore;穆寧;;實(shí)時(shí)組織彈性成像對(duì)于睪丸病變的評(píng)估[J];國(guó)際醫(yī)學(xué)放射學(xué)雜志;2012年03期

6 譚有娟;紀(jì)冬;牛小霞;王玲;馬艷琳;李亞;孟園;張健;陳國(guó)鳳;;瞬時(shí)彈性成像檢測(cè)肝硬度失敗的因素及分析[J];醫(yī)學(xué)研究雜志;2012年11期

7 黃浪;朱才義;;實(shí)時(shí)剪切波彈性成像的臨床應(yīng)用進(jìn)展[J];臨床超聲醫(yī)學(xué)雜志;2013年07期

8 J.H. Youk;H.M. Gweon;E.J. Son;J. Chung;J.A. Kim;E. Kim;楊淑杰;;三維剪切波彈性成像鑒別乳腺良惡性病變:與二維剪切波彈性成像的比較[J];國(guó)際醫(yī)學(xué)放射學(xué)雜志;2013年04期

9 張吉臻;胡兵;;前列腺剪切波彈性成像的初步臨床應(yīng)用體會(huì)[J];中華臨床醫(yī)師雜志(電子版);2013年10期

10 孫渭玲;嚴(yán)碧歌;馬磊;;超聲彈性成像技術(shù)及其應(yīng)用[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2007年09期

相關(guān)會(huì)議論文 前10條

1 馬蘇亞;;彈性成像技術(shù)在前列腺疾病中的應(yīng)用價(jià)值[A];2011年浙江省超聲醫(yī)學(xué)學(xué)術(shù)年會(huì)論文匯編[C];2011年

2 郭倩;胡兵;姜立新;;彈性成像評(píng)價(jià)高強(qiáng)度聚焦超聲消融灶的初步研究[A];第二屆長(zhǎng)三角超聲醫(yī)學(xué)論壇暨2009年浙江省超聲醫(yī)學(xué)學(xué)術(shù)年會(huì)論文匯編[C];2009年

3 郭倩;胡兵;姜立新;;彈性成像評(píng)價(jià)高強(qiáng)度聚焦超聲消融灶的初步研究[A];中華醫(yī)學(xué)會(huì)第十次全國(guó)超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2009年

4 郭倩;胡兵;姜立新;;彈性成像評(píng)價(jià)高強(qiáng)度聚焦超聲消融灶的初步研究[A];慶祝中國(guó)超聲診斷50年暨第十屆全國(guó)超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2008年

5 王譯晗;趙夏夏;吳永勝;王海筠;苗英;吉新燕;崔麗瓊;張孟楠;;不同年齡人群正常肝臟彈性成像定量的相關(guān)性分析[A];中國(guó)超聲醫(yī)學(xué)工程學(xué)會(huì)第九屆全國(guó)腹部超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2012年

6 樂堅(jiān);周世崇;范亦武;陳敏;曾煒;常才;;剪切波彈性成像在甲狀腺中的應(yīng)用價(jià)值初步探討并國(guó)內(nèi)外研究進(jìn)展回顧[A];中華醫(yī)學(xué)會(huì)第十三次全國(guó)超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2013年

7 方玲;;實(shí)時(shí)組織彈性成像在肝腫瘤診斷及鑒別診斷中的應(yīng)用價(jià)值[A];中國(guó)超聲醫(yī)學(xué)工程學(xué)會(huì)第八屆全國(guó)腹部超聲學(xué)術(shù)會(huì)議論文匯編[C];2010年

8 鄭權(quán);孫璐璐;宋毅;秦石成;;實(shí)時(shí)剪切波彈性成像在前列腺良惡性病變?cè)\斷中的初步應(yīng)用[A];中國(guó)超聲醫(yī)學(xué)工程學(xué)會(huì)第九屆全國(guó)腹部超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2012年

9 曾婕;鄭榮琴;鄭劍;黃澤萍;吳濤;;剪切波彈性成像檢測(cè)肝臟彈性模量的可靠性研究[A];中華醫(yī)學(xué)會(huì)第十三次全國(guó)超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2013年

10 李藝;王燕;曾敏霞;欒艷艷;常婷;胡兵;;實(shí)時(shí)超聲彈性成像與聲輻射力脈沖波彈性成像在肝局灶性病變?cè)\斷中的比較研究[A];中國(guó)超聲醫(yī)學(xué)工程學(xué)會(huì)第九屆全國(guó)腹部超聲醫(yī)學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2012年

相關(guān)重要報(bào)紙文章 前1條

1 Yngve Vogt等 編譯 王迪;彈性測(cè)癌[N];醫(yī)藥經(jīng)濟(jì)報(bào);2013年

相關(guān)博士學(xué)位論文 前3條

1 李昶田;實(shí)時(shí)剪切波彈性成像對(duì)乳鼠腦功能影響的研究[D];中國(guó)人民解放軍醫(yī)學(xué)院;2016年

2 張啟芳;超聲內(nèi)鏡彈性成像對(duì)消化系實(shí)質(zhì)性腫瘤的診斷價(jià)值[D];廣西醫(yī)科大學(xué);2015年

3 張艷;前列腺周圍區(qū)病灶及良性前列腺增生的彈性成像特征研究[D];中國(guó)人民解放軍軍醫(yī)進(jìn)修學(xué)院;2011年

相關(guān)碩士學(xué)位論文 前10條

1 吳昊;剪切波彈性成像結(jié)合超聲造影診斷乳腺腫塊的多因素Logistic回歸分析[D];遵義醫(yī)學(xué)院;2015年

2 李琳;超聲實(shí)時(shí)組織彈性成像對(duì)甲狀腺?gòu)浡圆∽兊膽?yīng)用研究[D];川北醫(yī)學(xué)院;2015年

3 姚萌;實(shí)時(shí)剪切波彈性成像技術(shù)定量評(píng)價(jià)正常乳腺組織[D];廣西醫(yī)科大學(xué);2015年

4 朱林娜;常規(guī)超聲聯(lián)合實(shí)時(shí)組織彈性成像評(píng)分診斷乳腺良惡性病變的臨床研究[D];蘇州大學(xué);2015年

5 任海云;常規(guī)超聲聯(lián)合彈性成像診斷乳腺腫塊的價(jià)值[D];新鄉(xiāng)醫(yī)學(xué)院;2015年

6 李婭榮;實(shí)時(shí)彈性成像定量分析技術(shù)對(duì)慢性腎病診斷價(jià)值的初步研究[D];安徽醫(yī)科大學(xué);2015年

7 樊榮;剪切波彈性成像技術(shù)定量評(píng)價(jià)兔動(dòng)脈粥樣硬化斑塊組織成分及易損性的價(jià)值[D];山東大學(xué);2015年

8 楊艷梅;高頻彩超聯(lián)合多種彈性成像對(duì)甲狀腺良惡性結(jié)節(jié)診斷的應(yīng)用[D];昆明醫(yī)科大學(xué);2015年

9 張雁;BCS和慢性乙肝肝纖維化的彈性成像及病理對(duì)照研究[D];中國(guó)人民解放軍醫(yī)學(xué)院;2015年

10 任宇航;譜域光學(xué)相干層析及應(yīng)用的研究[D];北京理工大學(xué);2015年

，

本文編號(hào)：1898568